Restriction map for
pF1KA0972
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# NO CUT ENZYMES ( 151 enzymes) #
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Acc65I AccII AclI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsgI BsiEI BsiHKAI
BsiWI BsoBI Bsp1286I BspDI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FauI FseI FspI
HaeII HgaI HhaI HinP1I HpaI
KasI KpnI KspI MaeI MamI
MfeI MflI MluI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI TaqI Tth111I TthHB8I
Van91I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 1161 GACGTC GACGT^C
AccI 2001 GTCTAC GT^[AC][GT]AC
AccIII 1829 TCCGGA T^CCGGA
AciI 608 GCGG CCGC(-3/-1)
AcyI 1161 GACGTC G[GA]^CG[CT]C
AlwI 1057 GATCC GGATC(4/5)
AocI 1422 CCTCAGG CC^T[ACGT]AGG
AseI 571 ATTAAT AT^TAAT
AsnI 571 ATTAAT AT^TAAT
BcnI 1745 CCCGG CC^[GC]GG
BsaHI 1161 GACGTC G[GA]^CG[CT]C
BsaWI 1829 TCCGGA [AT]^CCGG[AT]
BseAI 1829 TCCGGA T^CCGGA
BsiYI 1158 CCAGACGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1158 CCAGACGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 247 GTCTC GTCTC(1/5)
BsmBI 246 CGTCTC CGTCTC(1/5)
BsmFI 1428 GGGAC GGGAC(10/14)
BsmI 1969 GAATGC GAATGC(1/-1)
Bsp1407I 209 TGTACA T^GTACA
BspEI 1829 TCCGGA T^CCGGA
BspHI 1137 TCATGA T^CATGA
BspMI 923 ACCTGC ACCTGC(4/8)
BsrDI 1973 GCAATG GCAATG(2/0)
BsrGI 209 TGTACA T^GTACA
BstNI 168 CCAGG CC^[AT]GG
Bsu36I 1422 CCTCAGG CC^T[ACGT]AGG
DraI 1798 TTTAAA TTT^AAA
Eco81I 1422 CCTCAGG CC^T[ACGT]AGG
EcoRII 168 CCAGG ^CC[AT]GG
EcoT14I 310 CCTTGG C^C[AT][AT]GG
Hin1I 1161 GACGTC G[GA]^CG[CT]C
MroI 1829 TCCGGA T^CCGGA
MspA1I 1067 CAGCTG C[AC]G^C[GT]G
MvaI 168 CCAGG CC^[AT]GG
MwoI 493 GCTGCTGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 1745 CCCGG CC^[GC]GG
NspI 486 GCATGT [GA]CATG^[CT]
PshBI 571 ATTAAT AT^TAAT
PvuII 1067 CAGCTG CAG^CTG
RcaI 1137 TCATGA T^CATGA
ScaI 1342 AGTACT AGT^ACT
SspBI 209 TGTACA T^GTACA
StyI 310 CCTTGG C^C[AT][AT]GG
SwaI 1797 ATTTAAAT ATTT^AAAT
XcmI 894 CCACAGGAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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BalI 1575 TGGCCA TGG^CCA
1911 TGGCCA
BclI 374 TGATCA T^GATCA
1848 TGATCA
BpmI 226 CTGGAG CTGGAG(16/14)
662 CTGGAG
BsaJI 167 CCCAGG C^C[ACGT][ACGT]GG
310 CCTTGG
BssKI 168 CCAGG ^CC[ACGT]GG
1745 CCCGG
Cac8I 414 GCAAGC GC[ACGT]^[ACGT]GC
753 GCAAGC
Cfr13I 51 GGGCC G^G[ACGT]CC
189 GGCCC
EaeI 1575 TGGCCA [CT]^GGCC[GA]
1911 TGGCCA
EarI 454 GAAGAG CTCTTC(1/4)
1078 GAAGAG
FbaI 374 TGATCA T^GATCA
1848 TGATCA
HapII 1746 CCGG C^CGG
1830 CCGG
HincII 960 GTCAAC GT[CT]^[GA]AC
1416 GTCAAC
HindII 960 GTCAAC GT[CT]^[GA]AC
1416 GTCAAC
HindIII 755 AAGCTT A^AGCTT
2010 AAGCTT
HpaII 1746 CCGG C^CGG
1830 CCGG
Ksp632I 454 GAAGAG CTCTTC(1/4)
1078 GAAGAG
MaeII 149 ACGT A^CGT
1162 ACGT
MluNI 1575 TGGCCA TGG^CCA
1911 TGGCCA
MscI 1575 TGGCCA TGG^CCA
1911 TGGCCA
MspI 1746 CCGG C^CGG
1830 CCGG
NlaIV 189 GGCCCC GG[ACGT]^[ACGT]CC
306 GGAGCC
PleI 1944 GACTC GAGTC(4/5)
342 GAGTC
Sau96I 51 GGGCC G^G[ACGT]CC
189 GGCCC
ScrFI 168 CCAGG CC^[ACGT]GG
1745 CCCGG
TfiI 204 GAATC G^A[AT]TC
2040 GAATC
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
BbvI BseRI BsrI BstF5I DdeI
DpnII EcoRI Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeIII MboI
MboII MnlI MseI MslI NdeII
NlaIII RsaI Sau3AI SfaNI SfcI
Tru9I TseI Tsp45I Tsp509I TspRI
XmnI