Restriction map for
pF1KA0950
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AviII AvrII
BamHI BbeI BbrPI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BsaAI BsaBI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstUI BstXI BstYI BstZ17I
CfoI Cfr10I ClaI CpoI DpnII
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MamI MboI MfeI MflI
MluI MroI MseI MunI MvnI
NaeI NarI NciI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI Sau3AI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp509I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 316 GTCTAC GT^[AC][GT]AC
AcyI 839 GACGCC G[GA]^CG[CT]C
AfaI 420 GTAC GT^AC
AocI 856 CCTGAGG CC^T[ACGT]AGG
BalI 722 TGGCCA TGG^CCA
BanI 174 GGTGCC G^G[CT][GA]CC
BbsI 631 GTCTTC GAAGAC(2/6)
BbvCI 95 CCTCAGC CCTCAGC(-5/-2)
BlpI 849 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 849 GCTGAGC GC^T[ACGT]AGC
BpuAI 631 GTCTTC GAAGAC(2/6)
BsaHI 839 GACGCC G[GA]^CG[CT]C
BsaI 248 GAGACC GGTCTC(1/5)
BsaWI 244 ACCGGA [AT]^CCGG[AT]
BseRI 859 GAGGAG GAGGAG(10/8)
BsmAI 248 GAGAC GTCTC(1/5)
BsmFI 40 GGGAC GGGAC(10/14)
BspHI 692 TCATGA T^CATGA
BspMI 57 GCAGGT ACCTGC(4/8)
BsrBI 782 GAGCGG CCGCTC(-3/-3)
Bsu36I 856 CCTGAGG CC^T[ACGT]AGG
CelII 849 GCTGAGC GC^T[ACGT]AGC
EaeI 722 TGGCCA [CT]^GGCC[GA]
Eco81I 856 CCTGAGG CC^T[ACGT]AGG
FauI 784 GCGGG CCCGC(4/6)
HapII 245 CCGG C^CGG
HgaI 839 GACGC GACGC(5/10)
Hin1I 839 GACGCC G[GA]^CG[CT]C
HpaII 245 CCGG C^CGG
MluNI 722 TGGCCA TGG^CCA
MscI 722 TGGCCA TGG^CCA
MspI 245 CCGG C^CGG
NcoI 533 CCATGG C^CATGG
PstI 922 CTGCAG CTGCA^G
RcaI 692 TCATGA T^CATGA
RsaI 420 GTAC GT^AC
SacI 256 GAGCTC GAGCT^C
SfcI 922 CTGCAG C^T[GA][CT]AG
XcmI 412 CCAGGCTGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AlwNI 337 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
820 CAGTTGCTG
AspHI 256 GAGCTC G[AT]GC[AT]^C
676 GTGCTC
AspI 382 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
394 GACTATGTC
BfaI 188 CTAG C^TAG
892 CTAG
BpmI 83 CTCCAG CTGGAG(16/14)
219 CTCCAG
BsiHKAI 256 GAGCTC G[AT]GC[AT]^C
676 GTGCTC
BstEII 345 GGTGACC G^GT[ACGT]ACC
832 GGTAACC
BstPI 345 GGTGACC G^GT[ACGT]ACC
832 GGTAACC
Cac8I 466 GCTTGC GC[ACGT]^[ACGT]GC
674 GCGTGC
DraII 32 AGGCCCC [GA]G^G[ACGT]CC[CT]
148 GGGGCCT
DsaI 20 CCGTGG C^C[GA][CT]GG
533 CCATGG
EarI 259 CTCTTC CTCTTC(1/4)
679 CTCTTC
EcoO109I 32 AGGCCCC [GA]G^G[ACGT]CC[CT]
148 GGGGCCT
EcoO65I 345 GGTGACC G^GT[ACGT]ACC
832 GGTAACC
EcoT14I 350 CCTTGG C^C[AT][AT]GG
533 CCATGG
Ksp632I 259 CTCTTC CTCTTC(1/4)
679 CTCTTC
MaeI 188 CTAG C^TAG
892 CTAG
PflFI 382 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
394 GACTATGTC
PvuII 273 CAGCTG CAG^CTG
337 CAGCTG
SapI 258 GCTCTTC GCTCTTC(1/4)
678 GCTCTTC
StyI 350 CCTTGG C^C[AT][AT]GG
533 CCATGG
TaqI 297 TCGA T^CGA
653 TCGA
TfiI 333 GATTC G^A[AT]TC
507 GATTC
Tth111I 382 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
394 GACTATGTC
TthHB8I 297 TCGA T^CGA
653 TCGA
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
Cfr13I DdeI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeIII
MboII MnlI MslI MspA1I MvaI
MwoI NlaIII NlaIV PleI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI