Restriction map for
pF1KA0944
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# NO CUT ENZYMES ( 41 enzymes) #
##################################
Aor51HI AscI BbeI BbvCI BcgI
BcnI BsiWI BsmBI BssHII BssSI
BstAPI CpoI DraIII Eco47III FseI
HaeII KasI KspI MluI NaeI
NarI NciI NgoMIV NotI NruI
PacI PmeI PvuI RsrII SacII
SalI SbfI SfiI SfoI SgrAI
SmaI SphI Sse8387I SwaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 1291 GACGTC GACGT^C
AccII 3697 CGCG CG^CG
AclI 263 AACGTT AA^CGTT
AcyI 1291 GACGTC G[GA]^CG[CT]C
AgeI 11656 ACCGGT A^CCGGT
AhdI 2992 GACATTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 10280 GTGCAC G^TGCAC
ApaLI 10280 GTGCAC G^TGCAC
AspEI 2992 GACATTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 9055 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 10964 TGCGCA TGC^GCA
BamHI 6983 GGATCC G^GATCC
BbrPI 2955 CACGTG CAC^GTG
BsaHI 1291 GACGTC G[GA]^CG[CT]C
BsaI 5277 GAGACC GGTCTC(1/5)
BspDI 5594 ATCGAT AT^CGAT
Bst1107I 6369 GTATAC GTA^TAC
BstEII 2584 GGTTACC G^GT[ACGT]ACC
BstPI 2584 GGTTACC G^GT[ACGT]ACC
BstUI 3697 CGCG CG^CG
BstZ17I 6369 GTATAC GTA^TAC
ClaI 5594 ATCGAT AT^CGAT
EagI 8366 CGGCCG C^GGCCG
Eam1105I 2992 GACATTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 8366 CGGCCG C^GGCCG
Eco52I 8366 CGGCCG C^GGCCG
EcoO65I 2584 GGTTACC G^GT[ACGT]ACC
FspI 10964 TGCGCA TGC^GCA
Hin1I 1291 GACGTC G[GA]^CG[CT]C
HpaI 7477 GTTAAC GTT^AAC
MvnI 3697 CGCG CG^CG
PaeR7I 689 CTCGAG C^TCGAG
PflFI 9055 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 11656 ACCGGT A^CCGGT
PmaCI 2955 CACGTG CAC^GTG
PmlI 2955 CACGTG CAC^GTG
PshAI 4424 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 263 AACGTT AA^CGTT
SacI 3151 GAGCTC GAGCT^C
SapI 3351 GAAGAGC GCTCTTC(1/4)
SnaBI 4449 TACGTA TAC^GTA
Tth111I 9055 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 689 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 6369 GTATAC GT^[AC][GT]AC
11448 GTCTAC
AccIII 8734 TCCGGA T^CCGGA
10686 TCCGGA
AflII 7149 CTTAAG C^TTAAG
8512 CTTAAG
ApaI 8208 GGGCCC GGGCC^C
10402 GGGCCC
AvaI 689 CTCGAG C^[CT]CG[GA]G
6149 CTCGGG
AvrII 3895 CCTAGG C^CTAGG
10791 CCTAGG
BalI 8534 TGGCCA TGG^CCA
11970 TGGCCA
BfrI 7149 CTTAAG C^TTAAG
8512 CTTAAG
BglI 153 GCCCCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
7063 GCCCACATGGC
BlnI 3895 CCTAGG C^CTAGG
10791 CCTAGG
BlpI 1513 GCTGAGC GC^T[ACGT]AGC
2098 GCTAAGC
Bpu1102I 1513 GCTGAGC GC^T[ACGT]AGC
2098 GCTAAGC
BsaBI 4600 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5587 GATGATGATC
BseAI 8734 TCCGGA T^CCGGA
10686 TCCGGA
BsiEI 455 CGACCG CG[GA][CT]^CG
8366 CGGCCG
BsoBI 689 CTCGAG C^[CT]CG[GA]G
6149 CTCGGG
BspEI 8734 TCCGGA T^CCGGA
10686 TCCGGA
BsrBI 9880 GAGCGG CCGCTC(-3/-3)
11944 GAGCGG
BstBI 6875 TTCGAA TT^CGAA
8879 TTCGAA
CelII 1513 GCTGAGC GC^T[ACGT]AGC
2098 GCTAAGC
EcoRI 5338 GAATTC G^AATTC
10720 GAATTC
HindIII 7605 AAGCTT A^AGCTT
8113 AAGCTT
MamI 4600 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5587 GATGATGATC
MluNI 8534 TGGCCA TGG^CCA
11970 TGGCCA
MroI 8734 TCCGGA T^CCGGA
10686 TCCGGA
MscI 8534 TGGCCA TGG^CCA
11970 TGGCCA
NdeI 2453 CATATG CA^TATG
8405 CATATG
NheI 2683 GCTAGC G^CTAGC
7855 GCTAGC
NspV 6875 TTCGAA TT^CGAA
8879 TTCGAA
SexAI 8591 ACCAGGT A^CC[AT]GGT
9694 ACCTGGT
SfuI 6875 TTCGAA TT^CGAA
8879 TTCGAA
SpeI 4846 ACTAGT A^CTAGT
6246 ACTAGT
StuI 8643 AGGCCT AGG^CCT
9527 AGGCCT
XbaI 6382 TCTAGA T^CTAGA
6435 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AseI AsnI Asp700I Asp718I AspHI
AvaII BanI BanII BbsI BbvI
BciVI BclI BfaI BglII BmyI
BpmI BpuAI BsaAI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DrdI DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoRV
EcoT14I EcoT22I FauI FbaI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MnlI MseI MslI MspA1I
MspI MunI MvaI MwoI NcoI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PshBI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI