Restriction map for
pF1KA0942
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# NO CUT ENZYMES ( 134 enzymes) #
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AccI AccII AccIII AclI AflII
AgeI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I AspI
AvaII AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmFI BspDI BspEI
BspLU11I BspMI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraII DraIII EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRII EcoRV EcoT22I FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HpaI KasI KspI
MamI MluI MroI MslI MvaI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI Sau96I SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SwaI Tth111I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 1282 GACGTC GACGT^C
Acc65I 127 GGTACC G^GTACC
AcyI 1282 GACGTC G[GA]^CG[CT]C
AhdI 684 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 937 GTGCAC G^TGCAC
ApaLI 937 GTGCAC G^TGCAC
Asp718I 127 GGTACC G^GTACC
AspEI 684 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1425 CTCGAG C^[CT]CG[GA]G
BanI 127 GGTACC G^G[CT][GA]CC
BbsI 804 GAAGAC GAAGAC(2/6)
BciVI 1446 GGATAC GTATCC(6/5)
BcnI 1443 CCCGG CC^[GC]GG
BpuAI 804 GAAGAC GAAGAC(2/6)
BsaHI 1282 GACGTC G[GA]^CG[CT]C
BsaI 103 GAGACC GGTCTC(1/5)
BsgI 1097 CTGCAC GTGCAG(16/14)
BsoBI 1425 CTCGAG C^[CT]CG[GA]G
BspHI 732 TCATGA T^CATGA
BsrBI 1245 CCGCTC CCGCTC(-3/-3)
BsrI 1010 ACTGG ACTGG(1/-1)
BssKI 1443 CCCGG ^CC[ACGT]GG
BstYI 558 GGATCT [GA]^GATC[CT]
DraI 316 TTTAAA TTT^AAA
DsaI 962 CCACGG C^C[GA][CT]GG
Eam1105I 684 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 1331 CCCGC CCCGC(4/6)
HapII 1444 CCGG C^CGG
HgaI 288 GACGC GACGC(5/10)
Hin1I 1282 GACGTC G[GA]^CG[CT]C
HindIII 598 AAGCTT A^AGCTT
HpaII 1444 CCGG C^CGG
KpnI 127 GGTACC GGTAC^C
MfeI 1074 CAATTG C^AATTG
MflI 558 GGATCT [GA]^GATC[CT]
MspI 1444 CCGG C^CGG
MunI 1074 CAATTG C^AATTG
NciI 1443 CCCGG CC^[GC]GG
NlaIV 127 GGTACC GG[ACGT]^[ACGT]CC
PaeR7I 1425 CTCGAG C^TCGAG
PstI 1403 CTGCAG CTGCA^G
RcaI 732 TCATGA T^CATGA
SapI 5 GCTCTTC GCTCTTC(1/4)
ScrFI 1443 CCCGG CC^[ACGT]GG
SfaNI 952 GCATC GCATC(5/9)
SfcI 1403 CTGCAG C^T[GA][CT]AG
SpeI 243 ACTAGT A^CTAGT
SspI 481 AATATT AAT^ATT
StuI 899 AGGCCT AGG^CCT
XcmI 131 CCAGCAGCAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1425 CTCGAG C^TCGAG
XhoII 558 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 986 ACGTGT A^C[GA][CT]GT
1481 ACGTGT
BalI 161 TGGCCA TGG^CCA
474 TGGCCA
BanII 3 GGGCTC G[GA]GC[CT]^C
1038 GAGCCC
BfaI 58 CTAG C^TAG
244 CTAG
BpmI 1011 CTGGAG CTGGAG(16/14)
1315 CTGGAG
BseRI 655 GAGGAG GAGGAG(10/8)
1183 GAGGAG
BsmI 322 GCATTC GAATGC(1/-1)
1352 GCATTC
Bsp1407I 575 TGTACA T^GTACA
752 TGTACA
BsrGI 575 TGTACA T^GTACA
752 TGTACA
Cac8I 1155 GCCTGC GC[ACGT]^[ACGT]GC
1233 GCTGGC
DrdI 1009 GACTGGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1261 GACAAGAAGGTC
MaeI 58 CTAG C^TAG
244 CTAG
MluNI 161 TGGCCA TGG^CCA
474 TGGCCA
MscI 161 TGGCCA TGG^CCA
474 TGGCCA
MspA1I 178 CAGCTG C[AC]G^C[GT]G
1085 CAGCTG
MwoI 952 GCATCCAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1233 GCTGGCCGAGC
PflMI 942 CCACGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1308 CCACTATCTGG
PvuII 178 CAGCTG CAG^CTG
1085 CAGCTG
ScaI 649 AGTACT AGT^ACT
703 AGTACT
SspBI 575 TGTACA T^GTACA
752 TGTACA
TaqI 1286 TCGA T^CGA
1426 TCGA
TfiI 188 GATTC G^A[AT]TC
764 GATTC
Tsp45I 300 GTCAC ^GT[GC]AC
1197 GTCAC
TspRI 84 CACTG CA[GC]TG[ACGT][ACGT]^
1461 CACTG
TthHB8I 1286 TCGA T^CGA
1426 TCGA
Van91I 942 CCACGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1308 CCACTATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BbvI BmyI BsaJI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrDI BstF5I BstXI DdeI DpnII
EaeI EarI EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI NdeII NlaIII PleI RsaI
Sau3AI SmlI StyI Tru9I TseI
Tsp509I