Restriction map for
pF1KA0790
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# NO CUT ENZYMES ( 70 enzymes) #
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Acc65I AccIII AflII AgeI AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbvCI
BcgI BfrI BglII BlnI BseAI
BsiWI BspEI BspHI BspLU11I BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FseI FspI HindIII
HpaI KpnI MluI MroI NdeI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
PvuI RcaI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 3418 GACGTC GACGT^C
AccI 2662 GTCGAC GT^[AC][GT]AC
AclI 702 AACGTT AA^CGTT
AocI 3730 CCTGAGG CC^T[ACGT]AGG
Asp700I 1525 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 682 TGGCCA TGG^CCA
BamHI 2113 GGATCC G^GATCC
BbeI 3141 GGCGCC GGCGC^C
BbrPI 874 CACGTG CAC^GTG
BciVI 149 GTATCC GTATCC(6/5)
BclI 599 TGATCA T^GATCA
BglI 3171 GCCCCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 144 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 84 ACCGGA [AT]^CCGG[AT]
BsiEI 2615 CGACCG CG[GA][CT]^CG
Bsp1407I 1304 TGTACA T^GTACA
BspMI 1213 ACCTGC ACCTGC(4/8)
BsrGI 1304 TGTACA T^GTACA
BssSI 2863 CACGAG CACGAG(-5/-1)
Bsu36I 3730 CCTGAGG CC^T[ACGT]AGG
CpoI 907 CGGTCCG CG^G[AT]CCG
Eco81I 3730 CCTGAGG CC^T[ACGT]AGG
EcoRI 885 GAATTC G^AATTC
EcoRV 1741 GATATC GAT^ATC
EcoT22I 2669 ATGCAT ATGCA^T
FbaI 599 TGATCA T^GATCA
KasI 3141 GGCGCC G^GCGCC
KspI 3599 CCGCGG CCGC^GG
MamI 144 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 605 CAATTG C^AATTG
MluNI 682 TGGCCA TGG^CCA
MscI 682 TGGCCA TGG^CCA
MslI 1821 CATCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 605 CAATTG C^AATTG
NarI 3141 GGCGCC GG^CGCC
NsiI 2669 ATGCAT ATGCA^T
NspI 1949 ACATGC [GA]CATG^[CT]
PmaCI 874 CACGTG CAC^GTG
PmlI 874 CACGTG CAC^GTG
Psp1406I 702 AACGTT AA^CGTT
RsrII 907 CGGTCCG CG^G[AT]CCG
SacII 3599 CCGCGG CCGC^GG
SalI 2662 GTCGAC G^TCGAC
SapI 1639 GAAGAGC GCTCTTC(1/4)
SexAI 2403 ACCTGGT A^CC[AT]GGT
SfoI 3141 GGCGCC GGC^GCC
SspBI 1304 TGTACA T^GTACA
StuI 3124 AGGCCT AGG^CCT
XbaI 3276 TCTAGA T^CTAGA
XcmI 2142 CCAGGGCCTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1525 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcsI 885 GAATTC [GA]^AATT[CT]
2936 AAATTC
Alw44I 928 GTGCAC G^TGCAC
1681 GTGCAC
ApaLI 928 GTGCAC G^TGCAC
1681 GTGCAC
ApoI 885 GAATTC [GA]^AATT[CT]
2936 AAATTC
BsaAI 874 CACGTG [CT]AC^GT[GA]
1825 TACGTG
BsmI 1445 GAATGC GAATGC(1/-1)
2924 GAATGC
BsrDI 2303 CATTGC GCAATG(2/0)
2672 CATTGC
BstAPI 2550 GCAAGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2919 GCAGAGAATGC
DraIII 2402 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
3217 CACGGTGTG
DrdI 753 GACTGAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2653 GACTCTGCTGTC
EcoT14I 1769 CCATGG C^C[AT][AT]GG
3011 CCATGG
HaeII 355 AGCGCC [GA]GCGC^[CT]
3141 GGCGCC
NaeI 3583 GCCGGC GCC^GGC
3658 GCCGGC
NcoI 1769 CCATGG C^CATGG
3011 CCATGG
NgoMIV 3583 GCCGGC G^CCGGC
3658 GCCGGC
NheI 298 GCTAGC G^CTAGC
2224 GCTAGC
PstI 1215 CTGCAG CTGCA^G
3698 CTGCAG
PvuII 17 CAGCTG CAG^CTG
316 CAGCTG
SacI 1554 GAGCTC GAGCT^C
3187 GAGCTC
SmaI 24 CCCGGG CCC^GGG
3263 CCCGGG
StyI 1769 CCATGG C^C[AT][AT]GG
3011 CCATGG
XmaCI 24 CCCGGG C^CCGGG
3263 CCCGGG
XmaI 24 CCCGGG C^CCGGG
3263 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspDI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I ClaI DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII