Restriction map for
pF1KA0700
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# NO CUT ENZYMES ( 77 enzymes) #
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AccIII AclI AflII AgeI AhdI
AocI AscI AseI AsnI AspEI
AviII AvrII BbvCI BciVI BfrI
BlnI BsaBI BsaWI BseAI BsiEI
BsmI Bsp1407I BspEI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I DraI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRV FspI
HincII HindII HindIII HpaI MamI
MfeI MluI MroI MunI NdeI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI SalI SbfI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AatII 2614 GACGTC GACGT^C
ApaI 2999 GGGCCC GGGCC^C
AvaI 84 CTCGGG C^[CT]CG[GA]G
BamHI 1192 GGATCC G^GATCC
BbrPI 3328 CACGTG CAC^GTG
BcgI 2859 GCACCTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2795 TGATCA T^GATCA
BglI 44 GCCCCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiWI 1340 CGTACG C^GTACG
BsmBI 1399 GAGACG CGTCTC(1/5)
BsoBI 84 CTCGGG C^[CT]CG[GA]G
BspDI 229 ATCGAT AT^CGAT
BspLU11I 3125 ACATGT A^CATGT
BsrDI 2580 CATTGC GCAATG(2/0)
BstXI 2420 CCAGTGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 229 ATCGAT AT^CGAT
CpoI 2332 CGGACCG CG^G[AT]CCG
DrdI 1276 GACACCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1172 ATGCAT ATGCA^T
FbaI 2795 TGATCA T^GATCA
FseI 604 GGCCGGCC GGCCGG^CC
KspI 47 CCGCGG CCGC^GG
NheI 3250 GCTAGC G^CTAGC
NsiI 1172 ATGCAT ATGCA^T
PmaCI 3328 CACGTG CAC^GTG
PmlI 3328 CACGTG CAC^GTG
RsrII 2332 CGGACCG CG^G[AT]CCG
SacII 47 CCGCGG CCGC^GG
ScaI 2441 AGTACT AGT^ACT
SexAI 146 ACCAGGT A^CC[AT]GGT
SfiI 43 GGCCCCGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2974 CGCCGGTG C[GA]^CCGG[CT]G
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# TWO CUT ENZYMES ( 23 enzymes) #
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Acc65I 868 GGTACC G^GTACC
3297 GGTACC
AccI 1795 GTCTAC GT^[AC][GT]AC
2215 GTCTAC
Asp700I 207 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2754 GAACTGCTTC
Asp718I 868 GGTACC G^GTACC
3297 GGTACC
BalI 650 TGGCCA TGG^CCA
1409 TGGCCA
BglII 1673 AGATCT A^GATCT
2294 AGATCT
BspHI 203 TCATGA T^CATGA
2798 TCATGA
DraIII 754 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
2599 CACCTCGTG
EcoNI 2475 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3255 CCTGGTGCAGG
EcoRI 384 GAATTC G^AATTC
481 GAATTC
KpnI 868 GGTACC GGTAC^C
3297 GGTACC
MluNI 650 TGGCCA TGG^CCA
1409 TGGCCA
MscI 650 TGGCCA TGG^CCA
1409 TGGCCA
MseI 352 TTAA T^TAA
515 TTAA
NcoI 2396 CCATGG C^CATGG
2558 CCATGG
NspI 620 GCATGT [GA]CATG^[CT]
3125 ACATGT
PflMI 1005 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2856 CCAGCACCTGG
RcaI 203 TCATGA T^CATGA
2798 TCATGA
SapI 954 GAAGAGC GCTCTTC(1/4)
264 GCTCTTC
TfiI 296 GATTC G^A[AT]TC
472 GATTC
Tru9I 352 TTAA T^TAA
515 TTAA
Van91I 1005 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2856 CCAGCACCTGG
XmnI 207 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2754 GAACTGCTTC
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# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
Aor51HI ApaLI ApoI AspHI AspI
AvaII BanI BanII BbeI BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco47III EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV PflFI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StyI TaqI TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XcmI
XhoII