Restriction map for
pF1KA0676
##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I BamHI BbrPI
BfrI BsaAI BsiWI BsmI Bsp1407I
BspDI BspHI BsrGI Bst1107I BstAPI
BstZ17I ClaI CpoI DraI Eco47III
EcoRI EcoT22I HindIII HpaI KpnI
KspI MfeI MunI NdeI NotI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SexAI
SgrAI SnaBI SpeI SspBI SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 2985 TCCGGA T^CCGGA
AcsI 2317 AAATTC [GA]^AATT[CT]
AhdI 3448 GACGACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 2317 AAATTC [GA]^AATT[CT]
AspEI 3448 GACGACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1332 TGCGCA TGC^GCA
AvrII 793 CCTAGG C^CTAGG
BbeI 1341 GGCGCC GGCGC^C
BciVI 1523 GTATCC GTATCC(6/5)
BclI 2693 TGATCA T^GATCA
BglII 2880 AGATCT A^GATCT
BlnI 793 CCTAGG C^CTAGG
BseAI 2985 TCCGGA T^CCGGA
BsiEI 2532 CGGCCG CG[GA][CT]^CG
BspEI 2985 TCCGGA T^CCGGA
BsrFI 2530 GCCGGC [GA]^CCGG[CT]
BssHII 3556 GCGCGC G^CGCGC
BstEII 1044 GGTGACC G^GT[ACGT]ACC
BstPI 1044 GGTGACC G^GT[ACGT]ACC
Cfr10I 2530 GCCGGC [GA]^CCGG[CT]
DraIII 924 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 199 GACTCCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2532 CGGCCG C^GGCCG
Eam1105I 3448 GACGACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2532 CGGCCG C^GGCCG
Eco52I 2532 CGGCCG C^GGCCG
EcoNI 1035 CCTGAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1044 GGTGACC G^GT[ACGT]ACC
EcoRV 316 GATATC GAT^ATC
FbaI 2693 TGATCA T^GATCA
FseI 2529 GGCCGGCC GGCCGG^CC
FspI 1332 TGCGCA TGC^GCA
KasI 1341 GGCGCC G^GCGCC
MluI 607 ACGCGT A^CGCGT
MroI 2985 TCCGGA T^CCGGA
NaeI 2530 GCCGGC GCC^GGC
NarI 1341 GGCGCC GG^CGCC
NgoMIV 2530 GCCGGC G^CCGGC
NheI 2497 GCTAGC G^CTAGC
PshAI 1047 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 2097 CCTGCAGG CCTGCA^GG
ScaI 2504 AGTACT AGT^ACT
SfoI 1341 GGCGCC GGC^GCC
SphI 1865 GCATGC GCATG^C
Sse8387I 2097 CCTGCAGG CCTGCA^GG
TfiI 350 GAATC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Asp700I 1368 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3195 GAAGAAGTTC
AspI 288 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3624 GACGGAGTC
BbvCI 1308 CCTCAGC CCTCAGC(-5/-2)
2025 CCTCAGC
BcgI 1686 CGAGCACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2997 GCACTACCTTCG
BsaBI 966 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2881 GATCTGGATC
BsaWI 2570 ACCGGA [AT]^CCGG[AT]
2985 TCCGGA
BsmBI 389 GAGACG CGTCTC(1/5)
3040 GAGACG
BspLU11I 3107 ACATGT A^CATGT
3452 ACATGT
BsrDI 1783 GCAATG GCAATG(2/0)
3495 GCAATG
BstBI 1924 TTCGAA TT^CGAA
3005 TTCGAA
HaeII 1341 GGCGCC [GA]GCGC^[CT]
2047 AGCGCC
MaeII 140 ACGT A^CGT
877 ACGT
MamI 966 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2881 GATCTGGATC
MseI 2480 TTAA T^TAA
3047 TTAA
NcoI 1391 CCATGG C^CATGG
3125 CCATGG
NspV 1924 TTCGAA TT^CGAA
3005 TTCGAA
PflFI 288 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3624 GACGGAGTC
PflMI 2382 CCAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3372 CCAGGTGGTGG
SalI 2062 GTCGAC G^TCGAC
3574 GTCGAC
SfiI 732 GGCCAACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
797 GGCCCATCCGGCC
SfuI 1924 TTCGAA TT^CGAA
3005 TTCGAA
SmaI 400 CCCGGG CCC^GGG
1594 CCCGGG
StuI 1832 AGGCCT AGG^CCT
3539 AGGCCT
Tru9I 2480 TTAA T^TAA
3047 TTAA
Tsp509I 472 AATT ^AATT
2318 AATT
Tth111I 288 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3624 GACGGAGTC
Van91I 2382 CCAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3372 CCAGGTGGTGG
XmaCI 400 CCCGGG C^CCGGG
1594 CCCGGG
XmaI 400 CCCGGG C^CCGGG
1594 CCCGGG
XmnI 1368 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3195 GAAGAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TseI Tsp45I
TspRI TthHB8I XcmI XhoII