Restriction map for
pF1KA0675
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AviII BamHI BanI BbeI
BbrPI BbvCI BcgI BfrI BsaHI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BsrBI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoT22I FauI FseI FspI Hin1I
KasI KpnI KspI MfeI MluI
MroI MunI MvnI NarI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SpeI SphI Sse8387I StuI Van91I
XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AflIII 3547 ACATGT A^C[GA][CT]GT
AocI 2775 CCTCAGG CC^T[ACGT]AGG
AspHI 569 GTGCTC G[AT]GC[AT]^C
AspI 2026 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2967 CCCGGG C^[CT]CG[GA]G
AvrII 1704 CCTAGG C^CTAGG
BalI 2988 TGGCCA TGG^CCA
BciVI 897 GTATCC GTATCC(6/5)
BclI 912 TGATCA T^GATCA
BglI 2740 GCCATTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1704 CCTAGG C^CTAGG
BlpI 2201 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2201 GCTCAGC GC^T[ACGT]AGC
BsaAI 2542 TACGTA [CT]AC^GT[GA]
BsaI 1558 GGTCTC GGTCTC(1/5)
BsiHKAI 569 GTGCTC G[AT]GC[AT]^C
BsoBI 2967 CCCGGG C^[CT]CG[GA]G
Bsp1407I 952 TGTACA T^GTACA
BspLU11I 3547 ACATGT A^CATGT
BspMI 3067 GCAGGT ACCTGC(4/8)
BsrFI 3605 GCCGGC [GA]^CCGG[CT]
BsrGI 952 TGTACA T^GTACA
BstEII 3595 GGTCACC G^GT[ACGT]ACC
BstPI 3595 GGTCACC G^GT[ACGT]ACC
Bsu36I 2775 CCTCAGG CC^T[ACGT]AGG
CelII 2201 GCTCAGC GC^T[ACGT]AGC
Cfr10I 3605 GCCGGC [GA]^CCGG[CT]
DsaI 3526 CCATGG C^C[GA][CT]GG
EaeI 2988 TGGCCA [CT]^GGCC[GA]
Eco81I 2775 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3595 GGTCACC G^GT[ACGT]ACC
EcoRI 3586 GAATTC G^AATTC
FbaI 912 TGATCA T^GATCA
HaeII 3036 AGCGCC [GA]GCGC^[CT]
HpaI 142 GTTAAC GTT^AAC
MluNI 2988 TGGCCA TGG^CCA
MscI 2988 TGGCCA TGG^CCA
MspA1I 2204 CAGCTG C[AC]G^C[GT]G
NaeI 3605 GCCGGC GCC^GGC
NcoI 3526 CCATGG C^CATGG
NdeI 2036 CATATG CA^TATG
NgoMIV 3605 GCCGGC G^CCGGC
NspI 3547 ACATGT [GA]CATG^[CT]
PflFI 2026 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 2204 CAGCTG CAG^CTG
SmaI 2967 CCCGGG CCC^GGG
SmlI 2826 CTTGAG C^T[CT][GA]AG
SnaBI 2542 TACGTA TAC^GTA
SspBI 952 TGTACA T^GTACA
SwaI 1427 ATTTAAAT ATTT^AAAT
TaqI 1224 TCGA T^CGA
Tth111I 2026 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1224 TCGA T^CGA
XbaI 2631 TCTAGA T^CTAGA
XmaCI 2967 CCCGGG C^CCGGG
XmaI 2967 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwNI 464 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3035 CAGCGCCTG
BcnI 2967 CCCGG CC^[GC]GG
2968 CCGGG
BsaBI 495 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1681 GATTACCATC
BseRI 1253 CTCCTC GAGGAG(10/8)
2855 CTCCTC
BsmBI 2976 CGTCTC CGTCTC(1/5)
3047 GAGACG
BsmI 2736 GAATGC GAATGC(1/-1)
2770 GCATTC
BspHI 3092 TCATGA T^CATGA
3453 TCATGA
BsrDI 978 GCAATG GCAATG(2/0)
2140 GCAATG
DraII 1909 GGGACCT [GA]G^G[ACGT]CC[CT]
3381 GGGTCCT
EcoO109I 1909 GGGACCT [GA]G^G[ACGT]CC[CT]
3381 GGGTCCT
EcoRV 1077 GATATC GAT^ATC
1528 GATATC
HaeIII 2989 GGCC GG^CC
3365 GGCC
HapII 2968 CCGG C^CGG
3606 CCGG
HgaI 3049 GACGC GACGC(5/10)
2430 GCGTC
HincII 95 GTCAAC GT[CT]^[GA]AC
142 GTTAAC
HindII 95 GTCAAC GT[CT]^[GA]AC
142 GTTAAC
HindIII 612 AAGCTT A^AGCTT
1159 AAGCTT
HpaII 2968 CCGG C^CGG
3606 CCGG
MamI 495 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1681 GATTACCATC
MslI 1282 CACAAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1426 CATTTAAATG
MspI 2968 CCGG C^CGG
3606 CCGG
NciI 2967 CCCGG CC^[GC]GG
2968 CCGGG
PpuMI 1909 GGGACCT [GA]G^G[AT]CC[CT]
3381 GGGTCCT
PstI 1496 CTGCAG CTGCA^G
2957 CTGCAG
RcaI 3092 TCATGA T^CATGA
3453 TCATGA
SapI 2053 GAAGAGC GCTCTTC(1/4)
3433 GAAGAGC
SspI 388 AATATT AAT^ATT
1230 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
AvaII BanII BbsI BbvI BfaI
BglII BmyI BpmI BpuAI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EarI EcoRII EcoT14I
Fnu4HI FokI HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PleI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII XmnI