Restriction map for
pF1KA0605
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# NO CUT ENZYMES ( 80 enzymes) #
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AccI AccIII AcsI AflII AocI
ApoI AscI AseI AsnI AviII
AvrII BamHI BbsI BbvCI BciVI
BclI BfrI BglII BlnI BpuAI
BsaBI BseAI BspDI BspEI BspHI
Bst1107I BstBI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DraIII Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI MamI MfeI
MflI MluI MroI MseI MunI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PmeI PshAI PshBI
PvuI RcaI RsrII SalI SapI
ScaI SexAI SfuI SgrAI SmlI
SnaBI SpeI SphI SspI SwaI
Tru9I Tsp509I XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 2629 GACGTC GACGT^C
Acc65I 287 GGTACC G^GTACC
AclI 1597 AACGTT AA^CGTT
AgeI 1697 ACCGGT A^CCGGT
AhdI 67 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 2671 GATCC GGATC(4/5)
Aor51HI 1045 AGCGCT AGC^GCT
Asp700I 1407 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 287 GGTACC G^GTACC
AspEI 67 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1453 GGCGCC GGCGC^C
BfaI 760 CTAG C^TAG
BglI 1160 GCCACCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1548 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1548 GCTGAGC GC^T[ACGT]AGC
BsiEI 1622 CGGCCG CG[GA][CT]^CG
BsiWI 1760 CGTACG C^GTACG
BsmI 669 GAATGC GAATGC(1/-1)
BspLU11I 1691 ACATGT A^CATGT
BsrDI 15 GCAATG GCAATG(2/0)
BssHII 1663 GCGCGC G^CGCGC
CelII 1548 GCTGAGC GC^T[ACGT]AGC
DrdI 1276 GACCGCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1622 CGGCCG C^GGCCG
Eam1105I 67 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1321 GAAGAG CTCTTC(1/4)
EclXI 1622 CGGCCG C^GGCCG
Eco47III 1045 AGCGCT AGC^GCT
Eco52I 1622 CGGCCG C^GGCCG
EcoNI 1992 CCTGTGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 1453 GGCGCC G^GCGCC
KpnI 287 GGTACC GGTAC^C
Ksp632I 1321 GAAGAG CTCTTC(1/4)
KspI 1685 CCGCGG CCGC^GG
MaeI 760 CTAG C^TAG
NaeI 1228 GCCGGC GCC^GGC
NarI 1453 GGCGCC GG^CGCC
NcoI 866 CCATGG C^CATGG
NgoMIV 1228 GCCGGC G^CCGGC
NheI 759 GCTAGC G^CTAGC
NspI 1691 ACATGT [GA]CATG^[CT]
PinAI 1697 ACCGGT A^CCGGT
Psp1406I 1597 AACGTT AA^CGTT
SacI 1464 GAGCTC GAGCT^C
SacII 1685 CCGCGG CCGC^GG
SbfI 2834 CCTGCAGG CCTGCA^GG
SfiI 1159 GGCCACCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1453 GGCGCC GGC^GCC
Sse8387I 2834 CCTGCAGG CCTGCA^GG
TfiI 887 GAATC G^A[AT]TC
XmnI 1407 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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Alw44I 2088 GTGCAC G^TGCAC
2592 GTGCAC
ApaLI 2088 GTGCAC G^TGCAC
2592 GTGCAC
AspI 415 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2653 GACATCGTC
BsaWI 883 ACCGGA [AT]^CCGG[AT]
1697 ACCGGT
Bsp1407I 371 TGTACA T^GTACA
1982 TGTACA
BsrGI 371 TGTACA T^GTACA
1982 TGTACA
BstEII 693 GGTGACC G^GT[ACGT]ACC
2118 GGTGACC
BstPI 693 GGTGACC G^GT[ACGT]ACC
2118 GGTGACC
DpnII 2671 GATC ^GATC
2707 GATC
EcoO65I 693 GGTGACC G^GT[ACGT]ACC
2118 GGTGACC
HaeII 1045 AGCGCT [GA]GCGC^[CT]
1453 GGCGCC
HincII 75 GTCAAC GT[CT]^[GA]AC
1327 GTTGAC
HindII 75 GTCAAC GT[CT]^[GA]AC
1327 GTTGAC
MboI 2671 GATC ^GATC
2707 GATC
NdeII 2671 GATC ^GATC
2707 GATC
PflFI 415 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2653 GACATCGTC
PpuMI 262 AGGACCT [GA]G^G[AT]CC[CT]
1534 GGGTCCT
Sau3AI 2671 GATC ^GATC
2707 GATC
SspBI 371 TGTACA T^GTACA
1982 TGTACA
Tth111I 415 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2653 GACATCGTC
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# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbvI BcgI BcnI BmyI BpmI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PflMI
PleI PmaCI PmlI PstI PvuII
RsaI Sau96I ScrFI SfaNI SfcI
SmaI StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XmaCI XmaI