Restriction map for
pF1KA0581
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI Aor51HI ApaI AscI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BlnI BsaBI BsaI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BsrBI BsrDI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV FauI
FseI FspI HpaI KpnI KspI
MamI MluI MroI MvnI NaeI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmeI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 2371 GTCTAC GT^[AC][GT]AC
AcyI 287 GGCGCC G[GA]^CG[CT]C
Alw44I 22 GTGCAC G^TGCAC
AocI 139 CCTCAGG CC^T[ACGT]AGG
ApaLI 22 GTGCAC G^TGCAC
AseI 979 ATTAAT AT^TAAT
AsnI 979 ATTAAT AT^TAAT
AvaI 3475 CTCGAG C^[CT]CG[GA]G
BbeI 287 GGCGCC GGCGC^C
BbrPI 1805 CACGTG CAC^GTG
BbvCI 1831 CCTCAGC CCTCAGC(-5/-2)
BciVI 2118 GGATAC GTATCC(6/5)
BfaI 3337 CTAG C^TAG
BglI 1264 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2685 AGATCT A^GATCT
BsaHI 287 GGCGCC G[GA]^CG[CT]C
BsaWI 3296 TCCGGT [AT]^CCGG[AT]
BsmI 1845 GAATGC GAATGC(1/-1)
BsoBI 3475 CTCGAG C^[CT]CG[GA]G
Bsp1407I 632 TGTACA T^GTACA
BspHI 1961 TCATGA T^CATGA
BsrGI 632 TGTACA T^GTACA
BstXI 1014 CCAACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 139 CCTCAGG CC^T[ACGT]AGG
DraII 245 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 1131 CCATGG C^C[GA][CT]GG
Eco81I 139 CCTCAGG CC^T[ACGT]AGG
EcoNI 63 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 245 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 3487 GAATTC G^AATTC
EcoT22I 446 ATGCAT ATGCA^T
HaeII 287 GGCGCC [GA]GCGC^[CT]
HgaI 2352 GACGC GACGC(5/10)
Hin1I 287 GGCGCC G[GA]^CG[CT]C
HincII 3123 GTTGAC GT[CT]^[GA]AC
HindII 3123 GTTGAC GT[CT]^[GA]AC
HindIII 2423 AAGCTT A^AGCTT
KasI 287 GGCGCC G^GCGCC
MaeI 3337 CTAG C^TAG
NarI 287 GGCGCC GG^CGCC
NcoI 1131 CCATGG C^CATGG
NdeI 2405 CATATG CA^TATG
NsiI 446 ATGCAT ATGCA^T
PaeR7I 3475 CTCGAG C^TCGAG
PmaCI 1805 CACGTG CAC^GTG
PmlI 1805 CACGTG CAC^GTG
PpuMI 245 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 979 ATTAAT AT^TAAT
RcaI 1961 TCATGA T^CATGA
SapI 1836 GCTCTTC GCTCTTC(1/4)
ScaI 1277 AGTACT AGT^ACT
SexAI 2795 ACCTGGT A^CC[AT]GGT
SfoI 287 GGCGCC GGC^GCC
SspBI 632 TGTACA T^GTACA
StuI 1969 AGGCCT AGG^CCT
XhoI 3475 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 633 GTAC GT^AC
1278 GTAC
AflII 475 CTTAAG C^TTAAG
751 CTTAAG
AspHI 22 GTGCAC G[AT]GC[AT]^C
1054 GTGCTC
AvaII 246 GGACC G^G[AT]CC
1887 GGACC
BcgI 74 GCACCAAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2292 GCAAGCCATTCG
BcnI 5 CCGGG CC^[GC]GG
16 CCCGG
BfrI 475 CTTAAG C^TTAAG
751 CTTAAG
BlpI 1779 GCTTAGC GC^T[ACGT]AGC
2470 GCTAAGC
Bpu1102I 1779 GCTTAGC GC^T[ACGT]AGC
2470 GCTAAGC
BsgI 3319 GTGCAG GTGCAG(16/14)
3493 GTGCAG
BsiHKAI 22 GTGCAC G[AT]GC[AT]^C
1054 GTGCTC
BspLU11I 434 ACATGT A^CATGT
950 ACATGT
BssSI 331 CTCGTG CACGAG(-5/-1)
355 CTCGTG
BstAPI 24 GCACGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3039 GCAGCAGCTGC
CelII 1779 GCTTAGC GC^T[ACGT]AGC
2470 GCTAAGC
MfeI 1181 CAATTG C^AATTG
2476 CAATTG
MslI 309 CACAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2376 CATAGAAGTG
MspA1I 1007 CAGCTG C[AC]G^C[GT]G
3043 CAGCTG
MunI 1181 CAATTG C^AATTG
2476 CAATTG
NciI 5 CCGGG CC^[GC]GG
16 CCCGG
PshAI 3126 GACGGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3259 GACAAAAGTC
PstI 1106 CTGCAG CTGCA^G
3424 CTGCAG
PvuII 1007 CAGCTG CAG^CTG
3043 CAGCTG
RsaI 633 GTAC GT^AC
1278 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AflIII AluI AlwI
AlwNI ApoI BalI BanI BanII
BbsI BbvI BclI BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EarI EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII