Restriction map for
pF1KA0433
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AspEI AspI AviII AvrII BalI
BbeI BbrPI BbvCI BcgI BclI
BglI BlnI BlpI Bpu1102I BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
BsmI BspDI BspEI BspLU11I BsrBI
BsrFI BssHII BstAPI BstEII BstPI
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoRI FbaI FseI FspI HaeII
HpaI KasI KspI MluI MluNI
MroI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PvuI RsrII
SacII SalI ScaI SexAI SfaNI
SfiI SfoI SgrAI SmaI SpeI
SphI StuI Tth111I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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Acc65I 2962 GGTACC G^GTACC
AccI 3549 GTATAC GT^[AC][GT]AC
AccII 3298 CGCG CG^CG
AclI 2572 AACGTT AA^CGTT
AflII 1384 CTTAAG C^TTAAG
AflIII 1851 ACGTGT A^C[GA][CT]GT
AocI 2281 CCTCAGG CC^T[ACGT]AGG
AseI 873 ATTAAT AT^TAAT
AsnI 873 ATTAAT AT^TAAT
Asp718I 2962 GGTACC G^GTACC
AvaI 3205 CTCGGG C^[CT]CG[GA]G
BamHI 2625 GGATCC G^GATCC
BciVI 1599 GTATCC GTATCC(6/5)
BcnI 2683 CCGGG CC^[GC]GG
BfrI 1384 CTTAAG C^TTAAG
BglII 2896 AGATCT A^GATCT
BsoBI 3205 CTCGGG C^[CT]CG[GA]G
Bsp1407I 2484 TGTACA T^GTACA
BspHI 1869 TCATGA T^CATGA
BsrGI 2484 TGTACA T^GTACA
BssSI 863 CACGAG CACGAG(-5/-1)
Bst1107I 3549 GTATAC GTA^TAC
BstBI 2253 TTCGAA TT^CGAA
BstUI 3298 CGCG CG^CG
BstZ17I 3549 GTATAC GTA^TAC
Bsu36I 2281 CCTCAGG CC^T[ACGT]AGG
CfoI 3221 GCGC GCG^C
Eco81I 2281 CCTCAGG CC^T[ACGT]AGG
EcoNI 3160 CCTACTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1096 GATATC GAT^ATC
EcoT22I 1957 ATGCAT ATGCA^T
FauI 3181 GCGGG CCCGC(4/6)
HapII 2683 CCGG C^CGG
HhaI 3221 GCGC GCG^C
HinP1I 3221 GCGC G^CGC
HincII 1440 GTTGAC GT[CT]^[GA]AC
HindII 1440 GTTGAC GT[CT]^[GA]AC
HindIII 1918 AAGCTT A^AGCTT
HpaII 2683 CCGG C^CGG
KpnI 2962 GGTACC GGTAC^C
MfeI 1103 CAATTG C^AATTG
MspI 2683 CCGG C^CGG
MunI 1103 CAATTG C^AATTG
MvnI 3298 CGCG CG^CG
NciI 2683 CCGGG CC^[GC]GG
NsiI 1957 ATGCAT ATGCA^T
NspV 2253 TTCGAA TT^CGAA
PacI 1963 TTAATTAA TTAAT^TAA
PflMI 3343 CCAACCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 873 ATTAAT AT^TAAT
Psp1406I 2572 AACGTT AA^CGTT
PvuII 1721 CAGCTG CAG^CTG
RcaI 1869 TCATGA T^CATGA
SacI 2584 GAGCTC GAGCT^C
SapI 1580 GAAGAGC GCTCTTC(1/4)
SbfI 1549 CCTGCAGG CCTGCA^GG
SfuI 2253 TTCGAA TT^CGAA
SmlI 1384 CTTAAG C^T[CT][GA]AG
Sse8387I 1549 CCTGCAGG CCTGCA^GG
SspBI 2484 TGTACA T^GTACA
SspI 1030 AATATT AAT^ATT
SwaI 207 ATTTAAAT ATTT^AAAT
Van91I 3343 CCAACCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 259 CCAGTGGAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 2999 GGCGTC G[GA]^CG[CT]C
3289 GACGCC
BanII 2584 GAGCTC G[GA]GC[CT]^C
2928 GAGCCC
BsaAI 2102 TACGTA [CT]AC^GT[GA]
3546 TACGTA
BsaBI 1247 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2604 GATTGTTATC
BsaHI 2999 GGCGTC G[GA]^CG[CT]C
3289 GACGCC
BsaI 2766 GAGACC GGTCTC(1/5)
3170 GGTCTC
BsgI 569 GTGCAG GTGCAG(16/14)
3638 GTGCAG
BspMI 3119 ACCTGC ACCTGC(4/8)
3555 ACCTGC
Hin1I 2999 GGCGTC G[GA]^CG[CT]C
3289 GACGCC
HphI 1537 GGTGA GGTGA(8/7)
1785 GGTGA
MamI 1247 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2604 GATTGTTATC
MslI 79 CATGCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3195 CACCTCGGTG
MspA1I 1721 CAGCTG C[AC]G^C[GT]G
3482 CCGCTG
PstI 1550 CTGCAG CTGCA^G
1718 CTGCAG
SnaBI 2102 TACGTA TAC^GTA
3546 TACGTA
Tsp45I 467 GTGAC ^GT[GC]AC
1826 GTGAC
XbaI 2419 TCTAGA T^CTAGA
3390 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BanI
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DsaI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HgaI HinfI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MvaI
MwoI NcoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII
XmnI