Restriction map for
pF1KA0374
##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspHI AviII AvrII
BamHI BbrPI BcgI BclI BfrI
BglII BlnI BsaAI BsaBI BsaWI
BseAI BsiHKAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Bsu36I ClaI CpoI
DraI Eco47III Eco81I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MseI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 155 GTAGAC GT^[AC][GT]AC
AcyI 1475 GGCGCC G[GA]^CG[CT]C
AhdI 1153 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 624 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1153 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 580 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 88 CCCGGG C^[CT]CG[GA]G
BbeI 1475 GGCGCC GGCGC^C
BbsI 462 GAAGAC GAAGAC(2/6)
BciVI 92 GGATAC GTATCC(6/5)
BfaI 1249 CTAG C^TAG
BpuAI 462 GAAGAC GAAGAC(2/6)
BsaHI 1475 GGCGCC G[GA]^CG[CT]C
BsiEI 1436 CGGCCG CG[GA][CT]^CG
BsmBI 664 GAGACG CGTCTC(1/5)
BsoBI 88 CCCGGG C^[CT]CG[GA]G
BspMI 570 GCAGGT ACCTGC(4/8)
BstEII 805 GGTGACC G^GT[ACGT]ACC
BstPI 805 GGTGACC G^GT[ACGT]ACC
BstXI 1137 CCAGGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1413 GGATCT [GA]^GATC[CT]
EagI 1436 CGGCCG C^GGCCG
Eam1105I 1153 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1436 CGGCCG C^GGCCG
Eco52I 1436 CGGCCG C^GGCCG
EcoO65I 805 GGTGACC G^GT[ACGT]ACC
Hin1I 1475 GGCGCC G[GA]^CG[CT]C
KasI 1475 GGCGCC G^GCGCC
MaeI 1249 CTAG C^TAG
MaeII 31 ACGT A^CGT
MflI 1413 GGATCT [GA]^GATC[CT]
NaeI 1434 GCCGGC GCC^GGC
NarI 1475 GGCGCC GG^CGCC
NgoMIV 1434 GCCGGC G^CCGGC
PflFI 580 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 510 CCACCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 739 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 271 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 626 AGTACT AGT^ACT
SfiI 522 GGCCCAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1475 GGCGCC GGC^GCC
SmaI 88 CCCGGG CCC^GGG
SmlI 565 CTCAAG C^T[CT][GA]AG
TaqI 578 TCGA T^CGA
Tsp509I 247 AATT ^AATT
Tth111I 580 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 578 TCGA T^CGA
Van91I 510 CCACCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1214 CCAACTCAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1413 GGATCT [GA]^GATC[CT]
XmaCI 88 CCCGGG C^CCGGG
XmaI 88 CCCGGG C^CCGGG
XmnI 624 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 514 CGCG CG^CG
900 CGCG
AlwNI 352 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
1151 CAGACCCTG
BbvCI 80 CCTCAGC CCTCAGC(-5/-2)
841 GCTGAGG
BglI 523 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1589 GCCCAGCGGGC
BlpI 231 GCTCAGC GC^T[ACGT]AGC
1569 GCTGAGC
Bpu1102I 231 GCTCAGC GC^T[ACGT]AGC
1569 GCTGAGC
BsaI 952 GAGACC GGTCTC(1/5)
1208 GAGACC
BstUI 514 CGCG CG^CG
900 CGCG
CelII 231 GCTCAGC GC^T[ACGT]AGC
1569 GCTGAGC
DraIII 306 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
511 CACCGCGTG
DrdI 465 GACGCAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
790 GACCCGGCTGTC
EarI 225 CTCTTC CTCTTC(1/4)
1345 GAAGAG
EcoNI 534 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1156 CCTGAGTCAGG
HaeII 290 GGCGCT [GA]GCGC^[CT]
1475 GGCGCC
Ksp632I 225 CTCTTC CTCTTC(1/4)
1345 GAAGAG
MslI 132 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
681 CATGGAGGTG
MvnI 514 CGCG CG^CG
900 CGCG
NcoI 324 CCATGG C^CATGG
1340 CCATGG
NspI 479 GCATGC [GA]CATG^[CT]
1055 GCATGC
PstI 367 CTGCAG CTGCA^G
619 CTGCAG
SfcI 367 CTGCAG C^T[GA][CT]AG
619 CTGCAG
SphI 479 GCATGC GCATG^C
1055 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AvaII BalI BanI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MluNI
MnlI MscI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TseI Tsp45I
TspRI