Restriction map for
pF1KA0318
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AflIII AgeI AocI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BamHI BbrPI BbvCI BciVI BclI
BfrI BlnI BsaAI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraIII Eco81I EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI RsrII SacII
SalI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AclI 1567 AACGTT AA^CGTT
AcsI 468 GAATTC [GA]^AATT[CT]
AhdI 700 GACAACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1929 GGGCCC GGGCC^C
ApoI 468 GAATTC [GA]^AATT[CT]
AspEI 700 GACAACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1288 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2155 GGCGCC GGCGC^C
BcgI 834 CGACAACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 568 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 568 GCTGAGC GC^T[ACGT]AGC
BsaBI 434 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 421 TCCGGT [AT]^CCGG[AT]
BsmBI 2219 GAGACG CGTCTC(1/5)
BspHI 275 TCATGA T^CATGA
BsrBI 2581 CCGCTC CCGCTC(-3/-3)
BsrFI 1573 ACCGGC [GA]^CCGG[CT]
BsrI 1317 CCAGT ACTGG(1/-1)
BstEII 1494 GGTGACC G^GT[ACGT]ACC
BstPI 1494 GGTGACC G^GT[ACGT]ACC
CelII 568 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1573 ACCGGC [GA]^CCGG[CT]
DraI 2629 TTTAAA TTT^AAA
DrdI 3058 GACGTAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1640 CGGCCG C^GGCCG
Eam1105I 700 GACAACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1640 CGGCCG C^GGCCG
Eco52I 1640 CGGCCG C^GGCCG
EcoNI 1269 CCTCAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1494 GGTGACC G^GT[ACGT]ACC
EcoRI 468 GAATTC G^AATTC
KasI 2155 GGCGCC G^GCGCC
MamI 434 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 2630 TTAA T^TAA
NarI 2155 GGCGCC GG^CGCC
NspI 1910 ACATGC [GA]CATG^[CT]
PflFI 1288 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 48 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1567 AACGTT AA^CGTT
PvuI 338 CGATCG CGAT^CG
RcaI 275 TCATGA T^CATGA
SapI 2536 GAAGAGC GCTCTTC(1/4)
SbfI 1447 CCTGCAGG CCTGCA^GG
SfiI 2016 GGCCCGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2155 GGCGCC GGC^GCC
Sse8387I 1447 CCTGCAGG CCTGCA^GG
TfiI 2678 GATTC G^A[AT]TC
Tru9I 2630 TTAA T^TAA
Tth111I 1288 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 48 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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Alw44I 1143 GTGCAC G^TGCAC
1886 GTGCAC
Aor51HI 316 AGCGCT AGC^GCT
2425 AGCGCT
ApaLI 1143 GTGCAC G^TGCAC
1886 GTGCAC
BalI 654 TGGCCA TGG^CCA
1364 TGGCCA
BbsI 880 GAAGAC GAAGAC(2/6)
2071 GTCTTC
BglI 1542 GCCCACCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2017 GCCCGGGAGGC
BglII 439 AGATCT A^GATCT
1211 AGATCT
BpuAI 880 GAAGAC GAAGAC(2/6)
2071 GTCTTC
BstAPI 24 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1729 GCAGTTGCTGC
Eco47III 316 AGCGCT AGC^GCT
2425 AGCGCT
MluNI 654 TGGCCA TGG^CCA
1364 TGGCCA
MscI 654 TGGCCA TGG^CCA
1364 TGGCCA
NcoI 2012 CCATGG C^CATGG
2214 CCATGG
PvuII 28 CAGCTG CAG^CTG
2305 CAGCTG
SmaI 164 CCCGGG CCC^GGG
2018 CCCGGG
SmlI 2356 CTCAAG C^T[CT][GA]AG
3098 CTCAAG
XmaCI 164 CCCGGG C^CCGGG
2018 CCCGGG
XmaI 164 CCCGGG C^CCGGG
2018 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII