Restriction map for
pF1KA0261
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# NO CUT ENZYMES ( 76 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I ApaLI AscI
Asp718I AvaI AviII AvrII BamHI
BcgI BfrI BglI BlnI BsaWI
BseAI BsiEI BsiWI BsoBI BspEI
BspLU11I BssHII BssSI Bst1107I BstZ17I
CpoI DraIII DrdI EagI EclXI
Eco52I EcoRI EcoT22I FseI FspI
HgaI HindIII HpaI KpnI MfeI
MluI MroI MunI NcoI NotI
NruI NsiI PacI PaeR7I PinAI
PmeI PpuMI PshAI Psp1406I PvuI
RsrII SacI SalI SapI SbfI
SexAI SfiI SmaI SnaBI SpeI
Sse8387I SwaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 68 enzymes) #
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AcyI 53 GGCGCC G[GA]^CG[CT]C
AflIII 2272 ACACGT A^C[GA][CT]GT
AhdI 1753 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2395 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3172 CCTCAGG CC^T[ACGT]AGG
Aor51HI 3135 AGCGCT AGC^GCT
ApaI 9 GGGCCC GGGCC^C
AseI 741 ATTAAT AT^TAAT
AsnI 741 ATTAAT AT^TAAT
AspEI 1753 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 631 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1289 GGACC G^G[AT]CC
BanII 9 GGGCCC G[GA]GC[CT]^C
BbeI 53 GGCGCC GGCGC^C
BbrPI 2167 CACGTG CAC^GTG
BbvCI 2956 GCTGAGG CCTCAGC(-5/-2)
BciVI 1377 GGATAC GTATCC(6/5)
BclI 3425 TGATCA T^GATCA
BcnI 75 CCGGG CC^[GC]GG
BglII 2478 AGATCT A^GATCT
BlpI 2972 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2972 GCTTAGC GC^T[ACGT]AGC
BsaAI 2167 CACGTG [CT]AC^GT[GA]
BsaBI 1021 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 53 GGCGCC G[GA]^CG[CT]C
BsmBI 1444 CGTCTC CGTCTC(1/5)
BsmI 2327 GAATGC GAATGC(1/-1)
BspDI 1064 ATCGAT AT^CGAT
BspMI 125 ACCTGC ACCTGC(4/8)
BstAPI 1459 GCAGATATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2324 TTCGAA TT^CGAA
BstEII 1898 GGTCACC G^GT[ACGT]ACC
BstPI 1898 GGTCACC G^GT[ACGT]ACC
BstXI 368 CCACATTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3172 CCTCAGG CC^T[ACGT]AGG
CelII 2972 GCTTAGC GC^T[ACGT]AGC
ClaI 1064 ATCGAT AT^CGAT
Eam1105I 1753 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3135 AGCGCT AGC^GCT
Eco81I 3172 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1898 GGTCACC G^GT[ACGT]ACC
FbaI 3425 TGATCA T^GATCA
Hin1I 53 GGCGCC G[GA]^CG[CT]C
KasI 53 GGCGCC G^GCGCC
KspI 167 CCGCGG CCGC^GG
MamI 1021 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 53 GGCGCC GG^CGCC
NciI 75 CCGGG CC^[GC]GG
NdeI 2967 CATATG CA^TATG
NspI 3585 GCATGC [GA]CATG^[CT]
NspV 2324 TTCGAA TT^CGAA
PflFI 631 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1925 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2167 CACGTG CAC^GTG
PmlI 2167 CACGTG CAC^GTG
PshBI 741 ATTAAT AT^TAAT
PstI 1421 CTGCAG CTGCA^G
PvuII 2414 CAGCTG CAG^CTG
SacII 167 CCGCGG CCGC^GG
SfoI 53 GGCGCC GGC^GCC
SfuI 2324 TTCGAA TT^CGAA
SgrAI 40 CACCGGCG C[GA]^CCGG[CT]G
SmlI 3376 CTTGAG C^T[CT][GA]AG
SphI 3585 GCATGC GCATG^C
SspI 3809 AATATT AAT^ATT
StuI 1214 AGGCCT AGG^CCT
Tth111I 631 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1925 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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Asp700I 2172 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2932 GAACTGATTC
BalI 206 TGGCCA TGG^CCA
3780 TGGCCA
BanI 53 GGCGCC G^G[CT][GA]CC
1204 GGCACC
BpmI 23 CTCCAG CTGGAG(16/14)
461 CTGGAG
BsaI 364 GAGACC GGTCTC(1/5)
3121 GGTCTC
BspHI 771 TCATGA T^CATGA
1554 TCATGA
DraII 8 AGGGCCC [GA]G^G[ACGT]CC[CT]
9 GGGCCCT
DsaI 167 CCGCGG C^C[GA][CT]GG
203 CCGTGG
EaeI 206 TGGCCA [CT]^GGCC[GA]
3780 TGGCCA
EcoNI 1254 CCTGAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3168 CCTACCTCAGG
EcoO109I 8 AGGGCCC [GA]G^G[ACGT]CC[CT]
9 GGGCCCT
EcoRV 415 GATATC GAT^ATC
988 GATATC
EcoT14I 1170 CCAAGG C^C[AT][AT]GG
2372 CCTTGG
HaeII 53 GGCGCC [GA]GCGC^[CT]
3135 AGCGCT
HincII 3262 GTCAAC GT[CT]^[GA]AC
3501 GTCAAC
HindII 3262 GTCAAC GT[CT]^[GA]AC
3501 GTCAAC
MluNI 206 TGGCCA TGG^CCA
3780 TGGCCA
MscI 206 TGGCCA TGG^CCA
3780 TGGCCA
MspA1I 167 CCGCGG C[AC]G^C[GT]G
2414 CAGCTG
NaeI 129 GCCGGC GCC^GGC
3589 GCCGGC
NgoMIV 129 GCCGGC G^CCGGC
3589 GCCGGC
NheI 2470 GCTAGC G^CTAGC
3399 GCTAGC
PleI 100 GAGTC GAGTC(4/5)
493 GAGTC
RcaI 771 TCATGA T^CATGA
1554 TCATGA
StyI 1170 CCAAGG C^C[AT][AT]GG
2372 CCTTGG
XbaI 2610 TCTAGA T^CTAGA
3796 TCTAGA
XmnI 2172 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2932 GAACTGATTC
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# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI BbsI BbvI
BfaI BmyI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BsrBI BsrDI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
EarI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SspBI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII