Restriction map for
pF1KA0236
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# NO CUT ENZYMES ( 72 enzymes) #
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AatII AccI AccIII AclI AcyI
AhdI AscI AseI AsnI AspEI
AvrII BbeI BcgI BglII BlnI
BsaBI BsaHI BseAI Bsp1407I BspDI
BspEI BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I EcoRV
FseI Hin1I HindIII HpaI KasI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NgoMIV NotI
NruI NspV PacI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SexAI SfiI SfoI
SfuI SnaBI SpeI SspBI SspI
SwaI XbaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcsI 781 GAATTC [GA]^AATT[CT]
Aor51HI 506 AGCGCT AGC^GCT
ApoI 781 GAATTC [GA]^AATT[CT]
Asp700I 591 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 4739 TGCGCA TGC^GCA
BbrPI 3130 CACGTG CAC^GTG
BbsI 1997 GAAGAC GAAGAC(2/6)
BciVI 3273 GGATAC GTATCC(6/5)
BclI 1491 TGATCA T^GATCA
BpuAI 1997 GAAGAC GAAGAC(2/6)
BsiWI 4917 CGTACG C^GTACG
BsmI 693 GAATGC GAATGC(1/-1)
BspHI 1188 TCATGA T^CATGA
BssSI 3580 CACGAG CACGAG(-5/-1)
BstEII 2029 GGTGACC G^GT[ACGT]ACC
BstPI 2029 GGTGACC G^GT[ACGT]ACC
BstXI 3702 CCAGACGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eco47III 506 AGCGCT AGC^GCT
EcoO65I 2029 GGTGACC G^GT[ACGT]ACC
EcoRI 781 GAATTC G^AATTC
FbaI 1491 TGATCA T^GATCA
FspI 4739 TGCGCA TGC^GCA
HaeII 506 AGCGCT [GA]GCGC^[CT]
HgaI 4725 GACGC GACGC(5/10)
NdeI 487 CATATG CA^TATG
NheI 2617 GCTAGC G^CTAGC
PaeR7I 3385 CTCGAG C^TCGAG
PmaCI 3130 CACGTG CAC^GTG
PmlI 3130 CACGTG CAC^GTG
RcaI 1188 TCATGA T^CATGA
ScaI 4625 AGTACT AGT^ACT
SgrAI 3565 CACCGGCG C[GA]^CCGG[CT]G
Tsp509I 782 AATT ^AATT
XhoI 3385 CTCGAG C^TCGAG
XmnI 591 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccII 2635 CGCG CG^CG
4727 CGCG
AflII 3447 CTTAAG C^TTAAG
3901 CTTAAG
AflIII 1370 ACATGT A^C[GA][CT]GT
3322 ACATGT
AgeI 3638 ACCGGT A^CCGGT
3737 ACCGGT
AspI 2944 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3004 GACCCAGTC
BalI 713 TGGCCA TGG^CCA
4257 TGGCCA
BamHI 4499 GGATCC G^GATCC
5013 GGATCC
BbvCI 789 CCTCAGC CCTCAGC(-5/-2)
2640 CCTCAGC
BfrI 3447 CTTAAG C^TTAAG
3901 CTTAAG
BsaAI 3130 CACGTG [CT]AC^GT[GA]
4915 CACGTA
BsiEI 894 CGACCG CG[GA][CT]^CG
4848 CGACCG
BspLU11I 1370 ACATGT A^CATGT
3322 ACATGT
BsrBI 5036 CCGCTC CCGCTC(-3/-3)
4171 GAGCGG
BsrDI 3215 GCAATG GCAATG(2/0)
4784 GCAATG
BstUI 2635 CGCG CG^CG
4727 CGCG
EcoT22I 721 ATGCAT ATGCA^T
1180 ATGCAT
MluNI 713 TGGCCA TGG^CCA
4257 TGGCCA
MscI 713 TGGCCA TGG^CCA
4257 TGGCCA
MvnI 2635 CGCG CG^CG
4727 CGCG
NsiI 721 ATGCAT ATGCA^T
1180 ATGCAT
PflFI 2944 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3004 GACCCAGTC
PinAI 3638 ACCGGT A^CCGGT
3737 ACCGGT
SapI 1108 GAAGAGC GCTCTTC(1/4)
2857 GAAGAGC
SmaI 137 CCCGGG CCC^GGG
4166 CCCGGG
Tth111I 2944 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3004 GACCCAGTC
XcmI 1599 CCAGCACCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3507 CCATCTACAGTGTGG
XmaCI 137 CCCGGG C^CCGGG
4166 CCCGGG
XmaI 137 CCCGGG C^CCGGG
4166 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
Acc65I AciI AfaI AluI Alw44I
AlwI AlwNI AocI ApaI ApaLI
Asp718I AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SmlI
SphI Sse8387I StuI StyI TaqI
TfiI Tru9I TseI Tsp45I TspRI
TthHB8I Van91I XhoII