Restriction map for
pF1KA0227
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BsaI BseAI
BsgI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BsrI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
HphI KasI KpnI Ksp632I MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MseI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SalI SapI
SbfI ScaI SexAI SfcI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tru9I Tsp45I Tth111I Van91I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcsI 214 AAATTC [GA]^AATT[CT]
AgeI 503 ACCGGT A^CCGGT
ApaI 323 GGGCCC GGGCC^C
ApoI 214 AAATTC [GA]^AATT[CT]
AspHI 154 GAGCTC G[AT]GC[AT]^C
AvaII 572 GGACC G^G[AT]CC
BlpI 421 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 421 GCTGAGC GC^T[ACGT]AGC
BsaWI 503 ACCGGT [AT]^CCGG[AT]
BsiHKAI 154 GAGCTC G[AT]GC[AT]^C
BsrBI 292 GAGCGG CCGCTC(-3/-3)
BssHII 303 GCGCGC G^CGCGC
BssSI 172 CACGAG CACGAG(-5/-1)
BstF5I 159 CATCC GGATG(2/0)
CelII 421 GCTGAGC GC^T[ACGT]AGC
DpnII 381 GATC ^GATC
DsaI 50 CCGCGG C^C[GA][CT]GG
FokI 159 CATCC GGATG(9/13)
HgaI 89 GCGTC GACGC(5/10)
KspI 50 CCGCGG CCGC^GG
MaeII 596 ACGT A^CGT
MaeIII 389 GTTAC ^GT[ACGT]AC
MboI 381 GATC ^GATC
MboII 471 GAAGA GAAGA(8/7)
MslI 591 CACCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 147 GCCGGC GCC^GGC
NdeII 381 GATC ^GATC
NgoMIV 147 GCCGGC G^CCGGC
NlaIII 663 CATG CATG^
PinAI 503 ACCGGT A^CCGGT
PpuMI 571 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 613 CAGCTG CAG^CTG
SacI 154 GAGCTC GAGCT^C
SacII 50 CCGCGG CCGC^GG
Sau3AI 381 GATC ^GATC
SfaNI 638 GATGC GCATC(5/9)
SfiI 141 GGCCGAGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 452 AATATT AAT^ATT
TfiI 600 GATTC G^A[AT]TC
Tsp509I 215 AATT ^AATT
TspRI 196 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BglI 142 GCCGAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
310 GCCTCCCCGGC
BpmI 415 CTCCAG CTGGAG(16/14)
253 CTGGAG
BseRI 107 GAGGAG GAGGAG(10/8)
349 GAGGAG
BsrFI 147 GCCGGC [GA]^CCGG[CT]
503 ACCGGT
Cfr10I 147 GCCGGC [GA]^CCGG[CT]
503 ACCGGT
DdeI 422 CTGAG C^T[ACGT]AG
631 CTCAG
EcoNI 342 CCTCTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
411 CCTGCTCCAGG
PleI 298 GACTC GAGTC(4/5)
443 GAGTC
SmaI 93 CCCGGG CCC^GGG
280 CCCGGG
TaqI 377 TCGA T^CGA
383 TCGA
TthHB8I 377 TCGA T^CGA
383 TCGA
XmaCI 93 CCCGGG C^CCGGG
280 CCCGGG
XmaI 93 CCCGGG C^CCGGG
280 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI AluI AvaI BanII
BbvI BcnI BmyI BsaJI BsiEI
BsiYI BslI BsmFI BsoBI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DraII EaeI EagI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIV Sau96I ScrFI StyI TseI