Restriction map for
pF1KA0104
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AviII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BciVI BclI BfrI BglII BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BseAI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI BsmFI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I CelII CfoI ClaI CpoI
DraI DraIII DrdI EaeI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HphI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MslI MunI
NarI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI StuI
SwaI Tsp45I Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccII 111 CGCG CG^CG
AgeI 177 ACCGGT A^CCGGT
AhdI 470 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 262 GATCC GGATC(4/5)
AlwNI 394 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 450 CCTTAGG CC^T[ACGT]AGG
Asp700I 235 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 470 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 132 GAGCAC G[AT]GC[AT]^C
AvaI 506 CTCGGG C^[CT]CG[GA]G
AvrII 42 CCTAGG C^CTAGG
BanII 148 GAGCCC G[GA]GC[CT]^C
BcgI 147 CGAGCCCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 151 CCCGG CC^[GC]GG
BfaI 43 CTAG C^TAG
BglI 117 GCCTGTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 42 CCTAGG C^CTAGG
BsiHKAI 132 GAGCAC G[AT]GC[AT]^C
BsmI 410 GCATTC GAATGC(1/-1)
BsoBI 506 CTCGGG C^[CT]CG[GA]G
BspMI 218 GCAGGT ACCTGC(4/8)
BstUI 111 CGCG CG^CG
BstYI 261 AGATCC [GA]^GATC[CT]
Bsu36I 450 CCTTAGG CC^T[ACGT]AGG
DraII 114 GGGGCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 470 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 425 GAAGAG CTCTTC(1/4)
Eco81I 450 CCTTAGG CC^T[ACGT]AGG
EcoO109I 114 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 235 GAATTC G^AATTC
HgaI 862 GCGTC GACGC(5/10)
Ksp632I 425 GAAGAG CTCTTC(1/4)
MaeI 43 CTAG C^TAG
MaeII 702 ACGT A^CGT
MaeIII 355 GTTAC ^GT[ACGT]AC
MflI 261 AGATCC [GA]^GATC[CT]
MvnI 111 CGCG CG^CG
NaeI 84 GCCGGC GCC^GGC
NciI 151 CCCGG CC^[GC]GG
NcoI 787 CCATGG C^CATGG
NdeI 371 CATATG CA^TATG
NgoMIV 84 GCCGGC G^CCGGC
NlaIII 788 CATG CATG^
PflMI 393 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 177 ACCGGT A^CCGGT
PstI 99 CTGCAG CTGCA^G
PvuII 362 CAGCTG CAG^CTG
SbfI 98 CCTGCAGG CCTGCA^GG
ScaI 312 AGTACT AGT^ACT
SfaNI 574 GATGC GCATC(5/9)
SmlI 225 CTTGAG C^T[CT][GA]AG
Sse8387I 98 CCTGCAGG CCTGCA^GG
SspI 715 AATATT AAT^ATT
Van91I 393 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 261 AGATCC [GA]^GATC[CT]
XmnI 235 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AfaI 313 GTAC GT^AC
622 GTAC
AvaII 105 GGTCC G^G[AT]CC
510 GGTCC
BsaWI 177 ACCGGT [AT]^CCGG[AT]
210 ACCGGA
BsrDI 12 CATTGC GCAATG(2/0)
34 GCAATG
BsrFI 84 GCCGGC [GA]^CCGG[CT]
177 ACCGGT
BstF5I 447 CATCC GGATG(2/0)
549 GGATG
Cfr10I 84 GCCGGC [GA]^CCGG[CT]
177 ACCGGT
DsaI 203 CCGTGG C^C[GA][CT]GG
787 CCATGG
EcoT14I 42 CCTAGG C^C[AT][AT]GG
787 CCATGG
FokI 447 CATCC GGATG(9/13)
549 GGATG
MspA1I 17 CAGCGG C[AC]G^C[GT]G
362 CAGCTG
PleI 69 GACTC GAGTC(4/5)
228 GAGTC
RsaI 313 GTAC GT^AC
622 GTAC
SfcI 99 CTGCAG C^T[GA][CT]AG
359 CTACAG
StyI 42 CCTAGG C^C[AT][AT]GG
787 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstNI Cac8I Cfr13I
DdeI DpnII EcoRII FauI Fnu4HI
HaeIII HapII HinfI HpaII ItaI
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIV Sau3AI
Sau96I ScrFI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I