##################################
# NO CUT ENZYMES (  206 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaJI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
Cfr13I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRV       EcoT14I     
EcoT22I     FauI        FokI        FseI        FspI        
HaeII       HaeIII      HapII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        HpaII       
HphI        KasI        KpnI        KspI        MaeI        
MaeII       MaeIII      MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MspA1I      MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScaI        
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        Tru9I       Tsp45I      Tth111I     
Van91I      XbaI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AciI            6  GCGG                 CCGC(-3/-1)
AcsI          199  GAATTC               [GA]^AATT[CT]
AluI          126  AGCT                 AG^CT
ApoI          199  GAATTC               [GA]^AATT[CT]
AvaI           56  CTCGAG               C^[CT]CG[GA]G
BanII          59  GAGCCC               G[GA]GC[CT]^C
BbvI           52  GCTGC                GCAGC(8/12)
BclI          309  TGATCA               T^GATCA
BmyI           59  GAGCCC               G[AGT]GC[ACT]^C
BsiYI          63  CCCCAGAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           63  CCCCAGAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         213  GTCCC                GGGAC(10/14)
BsoBI          56  CTCGAG               C^[CT]CG[GA]G
Bsp1286I       59  GAGCCC               G[AGT]GC[ACT]^C
BssKI          28  CCAGG                ^CC[ACGT]GG
BstNI          28  CCAGG                CC^[AT]GG
Cac8I         161  GCGTGC               GC[ACGT]^[ACGT]GC
CfoI          165  GCGC                 GCG^C
DdeI          270  CTTAG                C^T[ACGT]AG
DpnII         310  GATC                 ^GATC
EarI          250  GAAGAG               CTCTTC(1/4)
EcoRI         199  GAATTC               G^AATTC
EcoRII         28  CCAGG                ^CC[AT]GG
FbaI          309  TGATCA               T^GATCA
Fnu4HI         52  GCTGC                GC^[ACGT]GC
HhaI          165  GCGC                 GCG^C
HinP1I        165  GCGC                 G^CGC
ItaI           52  GCTGC                GC^[ACGT]GC
Ksp632I       250  GAAGAG               CTCTTC(1/4)
MboI          310  GATC                 ^GATC
MslI          313  CACACAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI           28  CCAGG                CC^[AT]GG
MwoI           52  GCTGCTCGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII         310  GATC                 ^GATC
NlaIII        293  CATG                 CATG^
NlaIV         149  GGAACC               GG[ACGT]^[ACGT]CC
NspI          292  GCATGT               [GA]CATG^[CT]
PaeR7I         56  CTCGAG               C^TCGAG
Sau3AI        310  GATC                 ^GATC
ScrFI          28  CCAGG                CC^[ACGT]GG
SmlI           56  CTCGAG               C^T[CT][GA]AG
TseI           52  GCTGC                G^C[AT]GC
XcmI          169  CCAACTCCCAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI           56  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (    2 enzymes) #
###################################

MboII         250  GAAGA                GAAGA(8/7)
               82  TCTTC               
TspRI          21  CAGTG                CA[GC]TG[ACGT][ACGT]^
              264  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (    6 enzymes) #
#########################################

HinfI       MnlI        TaqI        TfiI        Tsp509I     
TthHB8I