Restriction map for
pF1KA0044
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI Asp718I AspI AviII
BamHI BanI BbeI BbrPI BbsI
BbvCI BciVI BglI BglII BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiWI
BsmBI BsmFI BsmI BspDI BspEI
BspMI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DrdI Eco47III
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HhaI
Hin1I HinP1I KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 754 GTCTAC GT^[AC][GT]AC
AclI 345 AACGTT AA^CGTT
AflII 393 CTTAAG C^TTAAG
AflIII 275 ACATGT A^C[GA][CT]GT
AhdI 855 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1351 CCTCAGG CC^T[ACGT]AGG
AseI 664 ATTAAT AT^TAAT
AsnI 664 ATTAAT AT^TAAT
Asp700I 1152 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 855 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 497 CTCGGG C^[CT]CG[GA]G
AvrII 549 CCTAGG C^CTAGG
BcgI 1061 CGAAGATTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 327 CCCGG CC^[GC]GG
BfrI 393 CTTAAG C^TTAAG
BlnI 549 CCTAGG C^CTAGG
BsgI 784 GTGCAG GTGCAG(16/14)
BsiEI 1419 CGGCCG CG[GA][CT]^CG
BsoBI 497 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1192 TGTACA T^GTACA
BspHI 1034 TCATGA T^CATGA
BspLU11I 275 ACATGT A^CATGT
BsrBI 314 GAGCGG CCGCTC(-3/-3)
BsrGI 1192 TGTACA T^GTACA
BstBI 281 TTCGAA TT^CGAA
Bsu36I 1351 CCTCAGG CC^T[ACGT]AGG
DraII 1355 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 41 CCGTGG C^C[GA][CT]GG
EagI 1419 CGGCCG C^GGCCG
Eam1105I 855 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1419 CGGCCG C^GGCCG
Eco52I 1419 CGGCCG C^GGCCG
Eco81I 1351 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1355 AGGACCC [GA]G^G[ACGT]CC[CT]
HgaI 119 GCGTC GACGC(5/10)
HincII 271 GTTAAC GT[CT]^[GA]AC
HindII 271 GTTAAC GT[CT]^[GA]AC
HindIII 88 AAGCTT A^AGCTT
HpaI 271 GTTAAC GTT^AAC
MspA1I 766 CAGCTG C[AC]G^C[GT]G
NciI 327 CCCGG CC^[GC]GG
NdeI 259 CATATG CA^TATG
NspI 275 ACATGT [GA]CATG^[CT]
NspV 281 TTCGAA TT^CGAA
PflMI 36 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1355 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 664 ATTAAT AT^TAAT
Psp1406I 345 AACGTT AA^CGTT
PstI 1389 CTGCAG CTGCA^G
PvuII 766 CAGCTG CAG^CTG
RcaI 1034 TCATGA T^CATGA
SfuI 281 TTCGAA TT^CGAA
SspBI 1192 TGTACA T^GTACA
SspI 648 AATATT AAT^ATT
Van91I 36 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1367 CCAAGAAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1152 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 1092 GTAC GT^AC
1193 GTAC
BalI 847 TGGCCA TGG^CCA
962 TGGCCA
BanII 475 GAGCTC G[GA]GC[CT]^C
1004 GAGCTC
BfaI 550 CTAG C^TAG
1276 CTAG
BpmI 410 CTCCAG CTGGAG(16/14)
975 CTCCAG
BsmAI 408 GTCTC GTCTC(1/5)
973 GTCTC
BstF5I 760 CATCC GGATG(2/0)
9 GGATG
BstYI 492 AGATCC [GA]^GATC[CT]
1305 AGATCC
DraIII 146 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
733 CACAAAGTG
EcoT14I 549 CCTAGG C^C[AT][AT]GG
1376 CCTTGG
FokI 760 CATCC GGATG(9/13)
9 GGATG
HapII 328 CCGG C^CGG
954 CCGG
HpaII 328 CCGG C^CGG
954 CCGG
HphI 825 GGTGA GGTGA(8/7)
209 TCACC
MaeI 550 CTAG C^TAG
1276 CTAG
MaeII 108 ACGT A^CGT
346 ACGT
MflI 492 AGATCC [GA]^GATC[CT]
1305 AGATCC
MluNI 847 TGGCCA TGG^CCA
962 TGGCCA
MscI 847 TGGCCA TGG^CCA
962 TGGCCA
MslI 619 CATCATAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1161 CATGGAGATG
MspI 328 CCGG C^CGG
954 CCGG
PleI 855 GACTC GAGTC(4/5)
406 GAGTC
RsaI 1092 GTAC GT^AC
1193 GTAC
SacI 475 GAGCTC GAGCT^C
1004 GAGCTC
SapI 691 GAAGAGC GCTCTTC(1/4)
1155 GCTCTTC
SfaNI 10 GATGC GCATC(5/9)
618 GCATC
SfcI 370 CTACAG C^T[GA][CT]AG
1389 CTGCAG
SmlI 393 CTTAAG C^T[CT][GA]AG
555 CTTGAG
StyI 549 CCTAGG C^C[AT][AT]GG
1376 CCTTGG
TaqI 60 TCGA T^CGA
282 TCGA
TfiI 533 GAATC G^A[AT]TC
1065 GATTC
Tsp45I 152 GTGAC ^GT[GC]AC
1049 GTGAC
TthHB8I 60 TCGA T^CGA
282 TCGA
XhoII 492 AGATCC [GA]^GATC[CT]
1305 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI AvaII BbvI BclI BmyI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BsrI BssKI BstNI Cac8I Cfr13I
DdeI DpnII EaeI EarI EcoRII
FbaI Fnu4HI HaeIII HinfI ItaI
Ksp632I MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI Tru9I
TseI Tsp509I TspRI