Restriction map for
pF1KA0013
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AvaI AviII BamHI BanI BbeI
BbrPI BbvCI BciVI BcnI BglI
BsaAI BsaBI BsaHI BseAI BseRI
BsiWI BsmBI BsoBI Bsp1407I BspEI
BsrBI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstXI BstZ17I
CfoI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MroI
MslI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI Psp1406I
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 2829 GTCTAC GT^[AC][GT]AC
AgeI 1095 ACCGGT A^CCGGT
AocI 2967 CCTTAGG CC^T[ACGT]AGG
AseI 2533 ATTAAT AT^TAAT
AsnI 2533 ATTAAT AT^TAAT
AvrII 2329 CCTAGG C^CTAGG
BalI 2865 TGGCCA TGG^CCA
BanII 388 GAGCCC G[GA]GC[CT]^C
BcgI 84 GCAGTGCGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 2329 CCTAGG C^CTAGG
BlpI 976 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 976 GCTAAGC GC^T[ACGT]AGC
BsiEI 90 CGATCG CG[GA][CT]^CG
BsmI 2814 GAATGC GAATGC(1/-1)
BspHI 2901 TCATGA T^CATGA
BsrDI 986 CATTGC GCAATG(2/0)
BsrFI 1095 ACCGGT [GA]^CCGG[CT]
BstAPI 36 GCAGCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1292 TTCGAA TT^CGAA
Bsu36I 2967 CCTTAGG CC^T[ACGT]AGG
CelII 976 GCTAAGC GC^T[ACGT]AGC
Cfr10I 1095 ACCGGT [GA]^CCGG[CT]
EaeI 2865 TGGCCA [CT]^GGCC[GA]
Eco81I 2967 CCTTAGG CC^T[ACGT]AGG
EcoRI 2146 GAATTC G^AATTC
EcoT22I 701 ATGCAT ATGCA^T
MluNI 2865 TGGCCA TGG^CCA
MscI 2865 TGGCCA TGG^CCA
MspA1I 401 CAGCTG C[AC]G^C[GT]G
NsiI 701 ATGCAT ATGCA^T
NspV 1292 TTCGAA TT^CGAA
PflMI 178 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1095 ACCGGT A^CCGGT
PshBI 2533 ATTAAT AT^TAAT
PvuI 90 CGATCG CGAT^CG
PvuII 401 CAGCTG CAG^CTG
RcaI 2901 TCATGA T^CATGA
SfuI 1292 TTCGAA TT^CGAA
SpeI 1533 ACTAGT A^CTAGT
StuI 2332 AGGCCT AGG^CCT
Van91I 178 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1775 CCAAAGAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AflII 361 CTTAAG C^TTAAG
2491 CTTAAG
AflIII 2302 ACATGT A^C[GA][CT]GT
2368 ACATGT
AspHI 875 GAGCAC G[AT]GC[AT]^C
2633 GTGCTC
BclI 1178 TGATCA T^GATCA
2424 TGATCA
BfrI 361 CTTAAG C^TTAAG
2491 CTTAAG
BglII 1297 AGATCT A^GATCT
2852 AGATCT
BpmI 1523 CTCCAG CTGGAG(16/14)
2151 CTCCAG
BsaI 2973 GAGACC GGTCTC(1/5)
769 GGTCTC
BsaWI 1095 ACCGGT [AT]^CCGG[AT]
1550 ACCGGA
BsgI 332 CTGCAC GTGCAG(16/14)
2252 GTGCAG
BsiHKAI 875 GAGCAC G[AT]GC[AT]^C
2633 GTGCTC
BspDI 694 ATCGAT AT^CGAT
1111 ATCGAT
BspLU11I 2302 ACATGT A^CATGT
2368 ACATGT
BspMI 1677 ACCTGC ACCTGC(4/8)
1186 GCAGGT
ClaI 694 ATCGAT AT^CGAT
1111 ATCGAT
DsaI 78 CCGTGG C^C[GA][CT]GG
116 CCACGG
EarI 1075 CTCTTC CTCTTC(1/4)
2725 GAAGAG
FbaI 1178 TGATCA T^GATCA
2424 TGATCA
HapII 1096 CCGG C^CGG
1551 CCGG
HpaII 1096 CCGG C^CGG
1551 CCGG
Ksp632I 1075 CTCTTC CTCTTC(1/4)
2725 GAAGAG
MspI 1096 CCGG C^CGG
1551 CCGG
NspI 2302 ACATGT [GA]CATG^[CT]
2368 ACATGT
PstI 674 CTGCAG CTGCA^G
1184 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AvaII BbsI
BbvI BfaI BmyI BpuAI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HindIII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI