Restriction map for
pF1KA0003
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
Asp700I Asp718I AspEI AspHI AvaI
AvaII AviII AvrII BalI BamHI
BanI BbeI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaHI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CelII Cfr10I ClaI CpoI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HindIII KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AciI 1390 GCGG CCGC(-3/-1)
AcsI 495 GAATTC [GA]^AATT[CT]
AlwI 558 GATCC GGATC(4/5)
ApaI 1441 GGGCCC GGGCC^C
ApoI 495 GAATTC [GA]^AATT[CT]
AseI 910 ATTAAT AT^TAAT
AsnI 910 ATTAAT AT^TAAT
AspI 766 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 1441 GGGCCC G[GA]GC[CT]^C
BbrPI 217 CACGTG CAC^GTG
BsaAI 217 CACGTG [CT]AC^GT[GA]
BsaBI 862 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 445 GAGACC GGTCTC(1/5)
BseRI 400 CTCCTC GAGGAG(10/8)
BsmI 351 GAATGC GAATGC(1/-1)
BspLU11I 261 ACATGT A^CATGT
BsrDI 117 GCAATG GCAATG(2/0)
BstAPI 1107 GCAGTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 557 AGATCC [GA]^GATC[CT]
CfoI 66 GCGC GCG^C
DraI 1212 TTTAAA TTT^AAA
DraII 1440 AGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 1401 CCATGG C^C[GA][CT]GG
EcoO109I 1440 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 495 GAATTC G^AATTC
EcoT14I 1401 CCATGG C^C[AT][AT]GG
FauI 1390 GCGGG CCCGC(4/6)
HaeII 65 AGCGCC [GA]GCGC^[CT]
HhaI 66 GCGC GCG^C
HinP1I 66 GCGC G^CGC
HincII 1326 GTTAAC GT[CT]^[GA]AC
HindII 1326 GTTAAC GT[CT]^[GA]AC
HpaI 1326 GTTAAC GTT^AAC
HphI 1057 GGTGA GGTGA(8/7)
MamI 862 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 557 AGATCC [GA]^GATC[CT]
MslI 1464 CACAACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 484 CAGCTG C[AC]G^C[GT]G
NcoI 1401 CCATGG C^CATGG
PflFI 766 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 690 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 42 GACTC GAGTC(4/5)
PmaCI 217 CACGTG CAC^GTG
PmlI 217 CACGTG CAC^GTG
PshBI 910 ATTAAT AT^TAAT
PvuII 484 CAGCTG CAG^CTG
SspI 1061 AATATT AAT^ATT
StyI 1401 CCATGG C^C[AT][AT]GG
Tsp45I 1220 GTCAC ^GT[GC]AC
Tth111I 766 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 690 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1002 TCTAGA T^CTAGA
XhoII 557 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 216 ACACGT A^C[GA][CT]GT
261 ACATGT
BfaI 517 CTAG C^TAG
1003 CTAG
BmyI 1316 GTGCCC G[AGT]GC[ACT]^C
1441 GGGCCC
BsaJI 449 CCCAGG C^C[ACGT][ACGT]GG
1401 CCATGG
BsgI 54 GTGCAG GTGCAG(16/14)
857 GTGCAG
BsiYI 690 CCAGGAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1097 CCAGTCAGAGG
BslI 690 CCAGGAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1097 CCAGTCAGAGG
BsmAI 445 GAGAC GTCTC(1/5)
851 GAGAC
BsmFI 1228 GGGAC GGGAC(10/14)
1392 GGGAC
Bsp1286I 1316 GTGCCC G[AGT]GC[ACT]^C
1441 GGGCCC
BssKI 450 CCAGG ^CC[ACGT]GG
690 CCAGG
BstF5I 951 GGATG GGATG(2/0)
1336 GGATG
BstNI 450 CCAGG CC^[AT]GG
690 CCAGG
DpnII 558 GATC ^GATC
1254 GATC
EcoRII 450 CCAGG ^CC[AT]GG
690 CCAGG
FokI 951 GGATG GGATG(9/13)
1336 GGATG
MaeI 517 CTAG C^TAG
1003 CTAG
MaeII 218 ACGT A^CGT
1458 ACGT
MboI 558 GATC ^GATC
1254 GATC
MvaI 450 CCAGG CC^[AT]GG
690 CCAGG
NdeII 558 GATC ^GATC
1254 GATC
NspI 261 ACATGT [GA]CATG^[CT]
1112 ACATGC
PstI 199 CTGCAG CTGCA^G
413 CTGCAG
Sau3AI 558 GATC ^GATC
1254 GATC
ScrFI 450 CCAGG CC^[ACGT]GG
690 CCAGG
SmlI 298 CTTGAG C^T[CT][GA]AG
475 CTTGAG
TaqI 471 TCGA T^CGA
1479 TCGA
TthHB8I 471 TCGA T^CGA
1479 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI AluI AlwNI BbvI BpmI
BsaWI BsrI Cfr13I DdeI EarI
Fnu4HI HaeIII HapII HinfI HpaII
ItaI Ksp632I MaeIII MboII MnlI
MseI MspI MwoI NlaIII NlaIV
RsaI Sau96I SfaNI SfcI TfiI
Tru9I TseI Tsp509I TspRI