Restriction map for
pF1KB7875
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BbvI BcgI
BcnI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BsaI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CfoI
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FauI
Fnu4HI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI ItaI KasI KpnI KspI
MaeII MamI MfeI MflI MluI
MroI MslI MspA1I MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI StuI StyI SwaI
TseI Tth111I Van91I XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AfaI 78 GTAC GT^AC
BanII 277 GAGCCC G[GA]GC[CT]^C
BciVI 24 GTATCC GTATCC(6/5)
BclI 251 TGATCA T^GATCA
BlpI 163 GCTCAGC GC^T[ACGT]AGC
BmyI 277 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 163 GCTCAGC GC^T[ACGT]AGC
BseRI 139 GAGGAG GAGGAG(10/8)
BsmAI 294 GAGAC GTCTC(1/5)
BsmFI 1404 GTCCC GGGAC(10/14)
Bsp1286I 277 GAGCCC G[AGT]GC[ACT]^C
BspMI 223 GCAGGT ACCTGC(4/8)
BsrDI 147 GCAATG GCAATG(2/0)
BstAPI 223 GCAGGTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 644 GCATGC GC[ACGT]^[ACGT]GC
CelII 163 GCTCAGC GC^T[ACGT]AGC
EarI 291 GAAGAG CTCTTC(1/4)
FbaI 251 TGATCA T^GATCA
HphI 254 TCACC GGTGA(8/7)
Ksp632I 291 GAAGAG CTCTTC(1/4)
MwoI 223 GCAGGTATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PleI 1318 GAGTC GAGTC(4/5)
RsaI 78 GTAC GT^AC
SexAI 209 ACCTGGT A^CC[AT]GGT
SfaNI 47 GATGC GCATC(5/9)
SmlI 31 CTCAAG C^T[CT][GA]AG
SphI 644 GCATGC GCATG^C
SspI 525 AATATT AAT^ATT
TaqI 1240 TCGA T^CGA
Tsp45I 629 GTGAC ^GT[GC]AC
TthHB8I 1240 TCGA T^CGA
XbaI 261 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Asp700I 523 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
606 GAAGCCTTTC
BalI 119 TGGCCA TGG^CCA
352 TGGCCA
BbsI 214 GTCTTC GAAGAC(2/6)
1572 GTCTTC
BpuAI 214 GTCTTC GAAGAC(2/6)
1572 GTCTTC
DdeI 164 CTCAG C^T[ACGT]AG
239 CTAAG
DpnII 252 GATC ^GATC
310 GATC
EaeI 119 TGGCCA [CT]^GGCC[GA]
352 TGGCCA
MaeIII 322 GTTAC ^GT[ACGT]AC
629 GTGAC
MboI 252 GATC ^GATC
310 GATC
MluNI 119 TGGCCA TGG^CCA
352 TGGCCA
MscI 119 TGGCCA TGG^CCA
352 TGGCCA
NdeII 252 GATC ^GATC
310 GATC
NlaIV 96 GGGGCC GG[ACGT]^[ACGT]CC
1403 GGTCCC
NspI 297 ACATGC [GA]CATG^[CT]
644 GCATGC
Sau3AI 252 GATC ^GATC
310 GATC
TspRI 159 CACTG CA[GC]TG[ACGT][ACGT]^
499 CAGTG
XmnI 523 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
606 GAAGCCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AcsI AgeI AluI ApoI AvaII
BfaI BpmI BsaJI BsaWI BsgI
BsiYI BslI BsrFI BsrI BssKI
BstF5I BstNI Cfr10I Cfr13I EcoRI
EcoRII FokI HaeIII HapII HindIII
HinfI HpaII MaeI MboII MnlI
MseI MspI MvaI NlaIII PinAI
Sau96I ScrFI SfcI TfiI Tru9I
Tsp509I