Restriction map for
pF1KB7852
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaWI BseAI BseRI BsiHKAI
BsiWI BsmBI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I CelII
Cfr10I CpoI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRI EcoT22I FauI FbaI
FseI FspI HaeII Hin1I HincII
HindII HpaI KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI Sse8387I SspI
SwaI Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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ApaI 1746 GGGCCC GGGCC^C
Asp700I 528 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1717 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1144 CCCGGG C^[CT]CG[GA]G
BanI 1148 GGTGCC G^G[CT][GA]CC
BanII 1746 GGGCCC G[GA]GC[CT]^C
BfaI 667 CTAG C^TAG
BmyI 1746 GGGCCC G[AGT]GC[ACT]^C
BpmI 592 CTGGAG CTGGAG(16/14)
BsaBI 1731 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 1632 GTCTC GTCTC(1/5)
BsmI 1788 GCATTC GAATGC(1/-1)
BsoBI 1144 CCCGGG C^[CT]CG[GA]G
Bsp1286I 1746 GGGCCC G[AGT]GC[ACT]^C
BspDI 1681 ATCGAT AT^CGAT
BspMI 1467 GCAGGT ACCTGC(4/8)
BstEII 331 GGTTACC G^GT[ACGT]ACC
BstPI 331 GGTTACC G^GT[ACGT]ACC
BstYI 1112 AGATCC [GA]^GATC[CT]
CfoI 1462 GCGC GCG^C
ClaI 1681 ATCGAT AT^CGAT
DraI 862 TTTAAA TTT^AAA
DraII 1520 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 1520 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 331 GGTTACC G^GT[ACGT]ACC
EcoRV 1039 GATATC GAT^ATC
HgaI 65 GACGC GACGC(5/10)
HhaI 1462 GCGC GCG^C
HinP1I 1462 GCGC G^CGC
HindIII 25 AAGCTT A^AGCTT
MaeI 667 CTAG C^TAG
MamI 1731 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1112 AGATCC [GA]^GATC[CT]
MspA1I 134 CAGCGG C[AC]G^C[GT]G
NspI 1653 GCATGC [GA]CATG^[CT]
PflFI 1717 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1520 GGGACCT [GA]G^G[AT]CC[CT]
SapI 1648 GAAGAGC GCTCTTC(1/4)
SfaNI 1074 GCATC GCATC(5/9)
SmaI 1144 CCCGGG CCC^GGG
SpeI 666 ACTAGT A^CTAGT
SphI 1653 GCATGC GCATG^C
StuI 500 AGGCCT AGG^CCT
TaqI 1682 TCGA T^CGA
Tth111I 1717 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1682 TCGA T^CGA
XcmI 41 CCAAAGAGCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1112 AGATCC [GA]^GATC[CT]
XmaCI 1144 CCCGGG C^CCGGG
XmaI 1144 CCCGGG C^CCGGG
XmnI 528 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 136 GCGG CCGC(-3/-1)
319 GCGG
AocI 454 CCTAAGG CC^T[ACGT]AGG
1820 CCTCAGG
BsgI 140 CTGCAC GTGCAG(16/14)
445 CTGCAC
BsiEI 1049 CGATCG CG[GA][CT]^CG
1254 CGATCG
Bsp1407I 1377 TGTACA T^GTACA
1626 TGTACA
BsrGI 1377 TGTACA T^GTACA
1626 TGTACA
BstAPI 844 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1464 GCAGCAGGTGC
Bsu36I 454 CCTAAGG CC^T[ACGT]AGG
1820 CCTCAGG
Eco81I 454 CCTAAGG CC^T[ACGT]AGG
1820 CCTCAGG
HapII 1145 CCGG C^CGG
1578 CCGG
HpaII 1145 CCGG C^CGG
1578 CCGG
HphI 1356 GGTGA GGTGA(8/7)
1476 TCACC
MspI 1145 CCGG C^CGG
1578 CCGG
PleI 1294 GACTC GAGTC(4/5)
120 GAGTC
PvuI 1049 CGATCG CGAT^CG
1254 CGATCG
SfcI 314 CTGTAG C^T[GA][CT]AG
1323 CTGTAG
SspBI 1377 TGTACA T^GTACA
1626 TGTACA
TfiI 37 GATTC G^A[AT]TC
1288 GAATC
Tsp45I 122 GTCAC ^GT[GC]AC
1066 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AvaII BbvI BcnI BsaJI
BsiYI BslI BsmFI BsrI BssKI
BstF5I BstNI BstXI Cac8I Cfr13I
DdeI DpnII DsaI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI StyI
Tru9I TseI Tsp509I TspRI