Restriction map for
pF1KB7661
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AclI AcsI AcyI
AflII AflIII AgeI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BclI BfaI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsaHI BsiEI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI EarI EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MaeII MaeIII
MamI MfeI MluI MluNI MscI
MseI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tsp45I Tth111I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 982 GTCTAC GT^[AC][GT]AC
AccII 71 CGCG CG^CG
AccIII 953 TCCGGA T^CCGGA
AfaI 198 GTAC GT^AC
AhdI 61 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 994 GTGCAC G^TGCAC
AlwI 436 GATCC GGATC(4/5)
Aor51HI 578 AGCGCT AGC^GCT
ApaLI 994 GTGCAC G^TGCAC
AspEI 61 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 704 GGACC G^G[AT]CC
BanI 321 GGTGCC G^G[CT][GA]CC
BanII 450 GAGCCC G[GA]GC[CT]^C
BciVI 31 GTATCC GTATCC(6/5)
BlpI 912 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 912 GCTGAGC GC^T[ACGT]AGC
BsaI 109 GGTCTC GGTCTC(1/5)
BsaWI 953 TCCGGA [AT]^CCGG[AT]
BseAI 953 TCCGGA T^CCGGA
BspEI 953 TCCGGA T^CCGGA
BspMI 360 GCAGGT ACCTGC(4/8)
BsrDI 458 GCAATG GCAATG(2/0)
BsrFI 497 ACCGGC [GA]^CCGG[CT]
BstUI 71 CGCG CG^CG
BstYI 435 AGATCC [GA]^GATC[CT]
CelII 912 GCTGAGC GC^T[ACGT]AGC
Cfr10I 497 ACCGGC [GA]^CCGG[CT]
DpnII 436 GATC ^GATC
EaeI 795 TGGCCG [CT]^GGCC[GA]
Eam1105I 61 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 578 AGCGCT AGC^GCT
MboI 436 GATC ^GATC
MboII 833 TCTTC GAAGA(8/7)
MflI 435 AGATCC [GA]^GATC[CT]
MroI 953 TCCGGA T^CCGGA
MvnI 71 CGCG CG^CG
NdeII 436 GATC ^GATC
NspI 191 ACATGC [GA]CATG^[CT]
PaeR7I 112 CTCGAG C^TCGAG
PshAI 124 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 213 CAGCTG CAG^CTG
RsaI 198 GTAC GT^AC
Sau3AI 436 GATC ^GATC
SfiI 228 GGCCCCTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 15 CCCGGG CCC^GGG
StuI 518 AGGCCT AGG^CCT
TfiI 669 GATTC G^A[AT]TC
Tsp509I 840 AATT ^AATT
XhoI 112 CTCGAG C^TCGAG
XhoII 435 AGATCC [GA]^GATC[CT]
XmaCI 15 CCCGGG C^CCGGG
XmaI 15 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AocI 202 CCTGAGG CC^T[ACGT]AGG
259 CCTGAGG
BsmBI 117 GAGACG CGTCTC(1/5)
403 GAGACG
BsrBI 918 CCGCTC CCGCTC(-3/-3)
649 GAGCGG
BstXI 371 CCATGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
786 CCACAGCTATGG
Bsu36I 202 CCTGAGG CC^T[ACGT]AGG
259 CCTGAGG
DraII 158 AGGGCCT [GA]G^G[ACGT]CC[CT]
227 AGGCCCC
Eco81I 202 CCTGAGG CC^T[ACGT]AGG
259 CCTGAGG
EcoO109I 158 AGGGCCT [GA]G^G[ACGT]CC[CT]
227 AGGCCCC
HphI 428 TCACC GGTGA(8/7)
658 TCACC
PflMI 440 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
525 CCAGGAGCTGG
Van91I 440 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
525 CCAGGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AluI AlwNI AspHI AvaI
BbvI BcnI BglI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DsaI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MnlI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NlaIII NlaIV PleI Sau96I ScrFI
SfaNI SmlI StyI TaqI TseI
TspRI TthHB8I