##################################
# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI Eam1105I EarI Eco47III EcoO65I
EcoRV EcoT22I FbaI FokI FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I MaeI MaeII MaeIII
MamI MfeI MluI MroI MseI
MslI MunI NaeI NcoI NdeI
NgoMIV NheI NlaIII NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp45I Tth111I Van91I XbaI
XcmI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AcsI 534 GAATTC [GA]^AATT[CT]
AfaI 420 GTAC GT^AC
AlwI 615 GGATC GGATC(4/5)
AlwNI 418 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 440 GGGCCC GGGCC^C
ApoI 534 GAATTC [GA]^AATT[CT]
AspHI 259 GAGCAC G[AT]GC[AT]^C
BalI 153 TGGCCA TGG^CCA
BanI 323 GGCGCC G^G[CT][GA]CC
BanII 440 GGGCCC G[GA]GC[CT]^C
BbeI 323 GGCGCC GGCGC^C
BbsI 49 GTCTTC GAAGAC(2/6)
BglI 442 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 485 AGATCT A^GATCT
BpuAI 49 GTCTTC GAAGAC(2/6)
BseRI 687 CTCCTC GAGGAG(10/8)
BsiEI 27 CGGCCG CG[GA][CT]^CG
BsiHKAI 259 GAGCAC G[AT]GC[AT]^C
BspMI 252 ACCTGC ACCTGC(4/8)
BsrBI 319 GAGCGG CCGCTC(-3/-3)
BsrI 644 CCAGT ACTGG(1/-1)
EagI 27 CGGCCG C^GGCCG
EclXI 27 CGGCCG C^GGCCG
Eco52I 27 CGGCCG C^GGCCG
EcoNI 137 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 534 GAATTC G^AATTC
EcoT14I 623 CCAAGG C^C[AT][AT]GG
HgaI 34 GACGC GACGC(5/10)
HphI 467 TCACC GGTGA(8/7)
KasI 323 GGCGCC G^GCGCC
MboII 50 TCTTC GAAGA(8/7)
MluNI 153 TGGCCA TGG^CCA
MscI 153 TGGCCA TGG^CCA
NarI 323 GGCGCC GG^CGCC
PaeR7I 274 CTCGAG C^TCGAG
RsaI 420 GTAC GT^AC
SfaNI 681 GCATC GCATC(5/9)
SfoI 323 GGCGCC GGC^GCC
SmlI 274 CTCGAG C^T[CT][GA]AG
StyI 623 CCAAGG C^C[AT][AT]GG
TaqI 275 TCGA T^CGA
TspRI 645 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 275 TCGA T^CGA
XhoI 274 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 225 CGCG CG^CG
296 CGCG
AcyI 34 GACGCC G[GA]^CG[CT]C
323 GGCGCC
AocI 265 CCTCAGG CC^T[ACGT]AGG
328 CCTCAGG
AvaI 274 CTCGAG C^[CT]CG[GA]G
433 CCCGAG
BmyI 259 GAGCAC G[AGT]GC[ACT]^C
440 GGGCCC
BpmI 141 CTCCAG CTGGAG(16/14)
372 CTCCAG
BsaHI 34 GACGCC G[GA]^CG[CT]C
323 GGCGCC
BsmAI 197 GAGAC GTCTC(1/5)
582 GTCTC
BsoBI 274 CTCGAG C^[CT]CG[GA]G
433 CCCGAG
Bsp1286I 259 GAGCAC G[AGT]GC[ACT]^C
440 GGGCCC
BstUI 225 CGCG CG^CG
296 CGCG
BstYI 485 AGATCT [GA]^GATC[CT]
615 GGATCT
Bsu36I 265 CCTCAGG CC^T[ACGT]AGG
328 CCTCAGG
DpnII 486 GATC ^GATC
616 GATC
DsaI 224 CCGCGG C^C[GA][CT]GG
295 CCGCGG
EaeI 27 CGGCCG [CT]^GGCC[GA]
153 TGGCCA
Eco81I 265 CCTCAGG CC^T[ACGT]AGG
328 CCTCAGG
Hin1I 34 GACGCC G[GA]^CG[CT]C
323 GGCGCC
HinfI 199 GACTC G^A[ACGT]TC
283 GACTC
KspI 224 CCGCGG CCGC^GG
295 CCGCGG
MboI 486 GATC ^GATC
616 GATC
MflI 485 AGATCT [GA]^GATC[CT]
615 GGATCT
MvnI 225 CGCG CG^CG
296 CGCG
NdeII 486 GATC ^GATC
616 GATC
PleI 199 GACTC GAGTC(4/5)
283 GACTC
PpuMI 568 AGGACCT [GA]G^G[AT]CC[CT]
631 GGGACCC
PvuII 382 CAGCTG CAG^CTG
445 CAGCTG
SacII 224 CCGCGG CCGC^GG
295 CCGCGG
Sau3AI 486 GATC ^GATC
616 GATC
Tsp509I 130 AATT ^AATT
535 AATT
XhoII 485 AGATCT [GA]^GATC[CT]
615 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BsaJI BsiYI BslI BsmFI BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DraII EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HpaII ItaI MnlI MspA1I MspI
MvaI MwoI NciI NlaIV PstI
Sau96I ScrFI SfcI TseI