Restriction map for
pF1KSDB1489
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# NO CUT ENZYMES ( 82 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BfrI BglII
BlnI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI Eam1105I EcoO65I
EcoRI EcoRV EcoT22I FseI HgaI
HindIII HpaI KpnI KspI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SfiI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 24 enzymes) #
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BamHI 2409 GGATCC G^GATCC
BbrPI 3110 CACGTG CAC^GTG
BciVI 2980 GGATAC GTATCC(6/5)
BclI 1967 TGATCA T^GATCA
BsaBI 3535 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2491 GAGACC GGTCTC(1/5)
BsmBI 1989 CGTCTC CGTCTC(1/5)
BspLU11I 3773 ACATGT A^CATGT
EagI 3928 CGGCCG C^GGCCG
EclXI 3928 CGGCCG C^GGCCG
Eco52I 3928 CGGCCG C^GGCCG
FbaI 1967 TGATCA T^GATCA
MamI 3535 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1748 TTAA T^TAA
PaeR7I 1204 CTCGAG C^TCGAG
PmaCI 3110 CACGTG CAC^GTG
PmlI 3110 CACGTG CAC^GTG
SapI 1707 GCTCTTC GCTCTTC(1/4)
SbfI 1662 CCTGCAGG CCTGCA^GG
ScaI 191 AGTACT AGT^ACT
SphI 1622 GCATGC GCATG^C
Sse8387I 1662 CCTGCAGG CCTGCA^GG
Tru9I 1748 TTAA T^TAA
XhoI 1204 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AcyI 483 GGCGCC G[GA]^CG[CT]C
4415 GGCGCC
AflIII 1267 ACGTGT A^C[GA][CT]GT
3773 ACATGT
AocI 1481 CCTCAGG CC^T[ACGT]AGG
3664 CCTGAGG
Aor51HI 1787 AGCGCT AGC^GCT
2906 AGCGCT
BalI 50 TGGCCA TGG^CCA
1607 TGGCCA
BbeI 483 GGCGCC GGCGC^C
4415 GGCGCC
BbvCI 3675 CCTCAGC CCTCAGC(-5/-2)
742 GCTGAGG
BcgI 1196 GCAGCAACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2888 GCACCCGCCTCG
BsaHI 483 GGCGCC G[GA]^CG[CT]C
4415 GGCGCC
BsaWI 1342 ACCGGA [AT]^CCGG[AT]
4527 ACCGGA
BsiEI 3928 CGGCCG CG[GA][CT]^CG
4284 CGACCG
BsrDI 2416 CATTGC GCAATG(2/0)
770 GCAATG
BsrFI 598 GCCGGC [GA]^CCGG[CT]
655 GCCGGC
Bsu36I 1481 CCTCAGG CC^T[ACGT]AGG
3664 CCTGAGG
Cfr10I 598 GCCGGC [GA]^CCGG[CT]
655 GCCGGC
DraIII 889 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1906 CACCTGGTG
Eco47III 1787 AGCGCT AGC^GCT
2906 AGCGCT
Eco81I 1481 CCTCAGG CC^T[ACGT]AGG
3664 CCTGAGG
Hin1I 483 GGCGCC G[GA]^CG[CT]C
4415 GGCGCC
HincII 277 GTCAAC GT[CT]^[GA]AC
3574 GTCAAC
HindII 277 GTCAAC GT[CT]^[GA]AC
3574 GTCAAC
KasI 483 GGCGCC G^GCGCC
4415 GGCGCC
MluNI 50 TGGCCA TGG^CCA
1607 TGGCCA
MscI 50 TGGCCA TGG^CCA
1607 TGGCCA
NaeI 598 GCCGGC GCC^GGC
655 GCCGGC
NarI 483 GGCGCC GG^CGCC
4415 GGCGCC
NgoMIV 598 GCCGGC G^CCGGC
655 GCCGGC
SexAI 272 ACCTGGT A^CC[AT]GGT
1907 ACCTGGT
SfoI 483 GGCGCC GGC^GCC
4415 GGCGCC
SmlI 1204 CTCGAG C^T[CT][GA]AG
1924 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
AlwI AlwNI ApaI ApaLI AspHI
AvaI AvaII AviII BanI BanII
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI