Restriction map for
pF1KSDA1028
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BbrPI BbsI
BbvCI BcgI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaI
BseAI BsgI BsiEI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstF5I BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV FokI FseI FspI HindIII
HpaI KpnI KspI MfeI MluI
MluNI MroI MscI MspA1I MunI
NdeI NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 103 GTCTAC GT^[AC][GT]AC
AccII 85 CGCG CG^CG
AcsI 1069 GAATTC [GA]^AATT[CT]
AcyI 1182 GGCGCC G[GA]^CG[CT]C
AgeI 1499 ACCGGT A^CCGGT
ApoI 1069 GAATTC [GA]^AATT[CT]
Asp700I 246 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 1182 GGCGCC GGCGC^C
BciVI 413 GTATCC GTATCC(6/5)
BclI 747 TGATCA T^GATCA
BcnI 1241 CCGGG CC^[GC]GG
BglII 373 AGATCT A^GATCT
BsaBI 565 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1182 GGCGCC G[GA]^CG[CT]C
BseRI 545 CTCCTC GAGGAG(10/8)
BsmBI 1457 CGTCTC CGTCTC(1/5)
BspHI 744 TCATGA T^CATGA
BspMI 1225 GCAGGT ACCTGC(4/8)
BsrDI 239 CATTGC GCAATG(2/0)
BstAPI 981 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 85 CGCG CG^CG
EarI 1449 CTCTTC CTCTTC(1/4)
EcoNI 1290 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1069 GAATTC G^AATTC
EcoT14I 950 CCATGG C^C[AT][AT]GG
EcoT22I 146 ATGCAT ATGCA^T
FbaI 747 TGATCA T^GATCA
HaeII 1182 GGCGCC [GA]GCGC^[CT]
HgaI 83 GACGC GACGC(5/10)
Hin1I 1182 GGCGCC G[GA]^CG[CT]C
HincII 681 GTTGAC GT[CT]^[GA]AC
HindII 681 GTTGAC GT[CT]^[GA]AC
KasI 1182 GGCGCC G^GCGCC
Ksp632I 1449 CTCTTC CTCTTC(1/4)
MamI 565 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 223 CACCCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 85 CGCG CG^CG
NaeI 1039 GCCGGC GCC^GGC
NarI 1182 GGCGCC GG^CGCC
NciI 1241 CCGGG CC^[GC]GG
NcoI 950 CCATGG C^CATGG
NgoMIV 1039 GCCGGC G^CCGGC
NsiI 146 ATGCAT ATGCA^T
PinAI 1499 ACCGGT A^CCGGT
RcaI 744 TCATGA T^CATGA
SacI 1467 GAGCTC GAGCT^C
SexAI 1295 ACCAGGT A^CC[AT]GGT
SfoI 1182 GGCGCC GGC^GCC
StyI 950 CCATGG C^C[AT][AT]GG
XmnI 246 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AspHI 1434 GAGCAC G[AT]GC[AT]^C
1467 GAGCTC
BanII 599 GGGCTC G[GA]GC[CT]^C
1467 GAGCTC
BsaWI 1255 ACCGGA [AT]^CCGG[AT]
1499 ACCGGT
BsiHKAI 1434 GAGCAC G[AT]GC[AT]^C
1467 GAGCTC
BsmAI 449 GAGAC GTCTC(1/5)
1458 GTCTC
BsrBI 34 GAGCGG CCGCTC(-3/-3)
40 GAGCGG
BsrFI 1039 GCCGGC [GA]^CCGG[CT]
1499 ACCGGT
CfoI 1183 GCGC GCG^C
1337 GCGC
Cfr10I 1039 GCCGGC [GA]^CCGG[CT]
1499 ACCGGT
DsaI 950 CCATGG C^C[GA][CT]GG
1318 CCACGG
FauI 36 GCGGG CCCGC(4/6)
42 GCGGG
HhaI 1183 GCGC GCG^C
1337 GCGC
HinP1I 1183 GCGC G^CGC
1337 GCGC
MaeII 101 ACGT A^CGT
859 ACGT
PleI 1282 GACTC GAGTC(4/5)
503 GAGTC
PpuMI 486 AGGTCCT [GA]G^G[AT]CC[CT]
1010 AGGACCC
PstI 785 CTGCAG CTGCA^G
988 CTGCAG
SfcI 785 CTGCAG C^T[GA][CT]AG
988 CTGCAG
TspRI 264 CAGTG CA[GC]TG[ACGT][ACGT]^
703 CACTG
XcmI 399 CCAGATTCTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1431 CCAGAGCACACTTGG
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BamHI BanI BbvI BfaI BmyI
BpmI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI TfiI Tru9I TseI
Tsp45I Tsp509I XhoII