Restriction map for
pF1KB9517
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII Aor51HI AscI AseI AsnI
Asp718I AspI AviII AvrII BbvCI
BcgI BciVI BfaI BfrI BglI
BglII BlnI BseAI BsmI BspDI
BspEI BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI KpnI KspI MaeI
MfeI MluI MroI MseI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI TfiI Tru9I Tth111I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccII 823 CGCG CG^CG
AcsI 907 GAATTC [GA]^AATT[CT]
AcyI 542 GGCGCC G[GA]^CG[CT]C
AgeI 977 ACCGGT A^CCGGT
AhdI 1121 GACCCATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 907 GAATTC [GA]^AATT[CT]
Asp700I 862 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1121 GACCCATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 324 TGGCCA TGG^CCA
BamHI 936 GGATCC G^GATCC
BbeI 542 GGCGCC GGCGC^C
BclI 1190 TGATCA T^GATCA
BlpI 834 GCTCAGC GC^T[ACGT]AGC
BpmI 145 CTCCAG CTGGAG(16/14)
Bpu1102I 834 GCTCAGC GC^T[ACGT]AGC
BsaBI 660 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 542 GGCGCC G[GA]^CG[CT]C
BsaI 97 GGTCTC GGTCTC(1/5)
BsaWI 977 ACCGGT [AT]^CCGG[AT]
BsiEI 1136 CGGTCG CG[GA][CT]^CG
BsiWI 1253 CGTACG C^GTACG
BsmBI 357 CGTCTC CGTCTC(1/5)
Bsp1407I 1103 TGTACA T^GTACA
BsrDI 1518 CATTGC GCAATG(2/0)
BsrGI 1103 TGTACA T^GTACA
BstUI 823 CGCG CG^CG
CelII 834 GCTCAGC GC^T[ACGT]AGC
DraIII 1417 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1121 GACCCATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 862 GAAGAG CTCTTC(1/4)
EcoNI 894 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 907 GAATTC G^AATTC
EcoT14I 707 CCATGG C^C[AT][AT]GG
FbaI 1190 TGATCA T^GATCA
HgaI 372 GACGC GACGC(5/10)
Hin1I 542 GGCGCC G[GA]^CG[CT]C
KasI 542 GGCGCC G^GCGCC
Ksp632I 862 GAAGAG CTCTTC(1/4)
MamI 660 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 324 TGGCCA TGG^CCA
MscI 324 TGGCCA TGG^CCA
MvnI 823 CGCG CG^CG
NarI 542 GGCGCC GG^CGCC
NcoI 707 CCATGG C^CATGG
PaeR7I 221 CTCGAG C^TCGAG
PflMI 395 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 977 ACCGGT A^CCGGT
PpuMI 1119 GGGACCC [GA]G^G[AT]CC[CT]
SapI 862 GAAGAGC GCTCTTC(1/4)
SexAI 1418 ACCTGGT A^CC[AT]GGT
SfoI 542 GGCGCC GGC^GCC
SspBI 1103 TGTACA T^GTACA
StuI 1347 AGGCCT AGG^CCT
StyI 707 CCATGG C^C[AT][AT]GG
Tsp45I 336 GTCAC ^GT[GC]AC
Van91I 395 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 221 CTCGAG C^TCGAG
XmnI 862 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 71 ACATGT A^C[GA][CT]GT
755 ACATGT
Alw44I 759 GTGCAC G^TGCAC
1642 GTGCAC
ApaLI 759 GTGCAC G^TGCAC
1642 GTGCAC
AvaI 221 CTCGAG C^[CT]CG[GA]G
634 CCCGAG
BbrPI 806 CACGTG CAC^GTG
1581 CACGTG
BbsI 1386 GAAGAC GAAGAC(2/6)
1575 GAAGAC
BpuAI 1386 GAAGAC GAAGAC(2/6)
1575 GAAGAC
BsaAI 806 CACGTG [CT]AC^GT[GA]
1581 CACGTG
BsoBI 221 CTCGAG C^[CT]CG[GA]G
634 CCCGAG
BspLU11I 71 ACATGT A^CATGT
755 ACATGT
BspMI 830 ACCTGC ACCTGC(4/8)
1245 GCAGGT
BstYI 198 AGATCC [GA]^GATC[CT]
936 GGATCC
EaeI 324 TGGCCA [CT]^GGCC[GA]
1234 TGGCCG
FauI 821 CCCGC CCCGC(4/6)
1425 GCGGG
MaeIII 336 GTCAC ^GT[ACGT]AC
472 GTTAC
MflI 198 AGATCC [GA]^GATC[CT]
936 GGATCC
PmaCI 806 CACGTG CAC^GTG
1581 CACGTG
PmlI 806 CACGTG CAC^GTG
1581 CACGTG
SfaNI 165 GATGC GCATC(5/9)
1529 GCATC
SmlI 221 CTCGAG C^T[CT][GA]AG
1324 CTCAAG
Tsp509I 4 AATT ^AATT
908 AATT
XcmI 431 CCAGTCTGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
923 CCATGAACTTTGTGG
XhoII 198 AGATCC [GA]^GATC[CT]
936 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaII BanI
BanII BbvI BcnI BmyI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI Eco81I
EcoO109I EcoRII Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MboI
MboII MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfcI TaqI TseI TspRI TthHB8I