Restriction map for
pF1KB9515
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI Asp700I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspLU11I BsrBI BssHII
BstBI BstEII BstPI BstXI Bsu36I
CelII ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoT22I FauI FbaI FseI FspI
HindIII HpaI KasI KspI MaeII
MamI MroI MslI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SacII SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 860 GGTACC G^GTACC
AccII 517 CGCG CG^CG
AcyI 1447 GACGCC G[GA]^CG[CT]C
AflIII 516 ACGCGT A^C[GA][CT]GT
Aor51HI 1336 AGCGCT AGC^GCT
AseI 1405 ATTAAT AT^TAAT
AsnI 1405 ATTAAT AT^TAAT
Asp718I 860 GGTACC G^GTACC
AspHI 1234 GTGCTC G[AT]GC[AT]^C
BalI 1217 TGGCCA TGG^CCA
BanI 860 GGTACC G^G[CT][GA]CC
BbsI 726 GAAGAC GAAGAC(2/6)
BpuAI 726 GAAGAC GAAGAC(2/6)
BsaHI 1447 GACGCC G[GA]^CG[CT]C
BseRI 1010 CTCCTC GAGGAG(10/8)
BsiEI 38 CGACCG CG[GA][CT]^CG
BsiHKAI 1234 GTGCTC G[AT]GC[AT]^C
Bsp1407I 869 TGTACA T^GTACA
BspHI 450 TCATGA T^CATGA
BspMI 663 ACCTGC ACCTGC(4/8)
BsrDI 1505 GCAATG GCAATG(2/0)
BsrFI 80 ACCGGC [GA]^CCGG[CT]
BsrGI 869 TGTACA T^GTACA
BssSI 122 CTCGTG CACGAG(-5/-1)
Bst1107I 940 GTATAC GTA^TAC
BstUI 517 CGCG CG^CG
BstZ17I 940 GTATAC GTA^TAC
Cac8I 1289 GCATGC GC[ACGT]^[ACGT]GC
Cfr10I 80 ACCGGC [GA]^CCGG[CT]
DraI 484 TTTAAA TTT^AAA
EaeI 1217 TGGCCA [CT]^GGCC[GA]
Eco47III 1336 AGCGCT AGC^GCT
EcoRI 799 GAATTC G^AATTC
HaeII 1336 AGCGCT [GA]GCGC^[CT]
Hin1I 1447 GACGCC G[GA]^CG[CT]C
KpnI 860 GGTACC GGTAC^C
MfeI 17 CAATTG C^AATTG
MluI 516 ACGCGT A^CGCGT
MluNI 1217 TGGCCA TGG^CCA
MscI 1217 TGGCCA TGG^CCA
MunI 17 CAATTG C^AATTG
MvnI 517 CGCG CG^CG
NdeI 668 CATATG CA^TATG
PflMI 1063 CCATTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 729 GACTC GAGTC(4/5)
PshBI 1405 ATTAAT AT^TAAT
PvuII 1163 CAGCTG CAG^CTG
RcaI 450 TCATGA T^CATGA
SalI 33 GTCGAC G^TCGAC
ScaI 1305 AGTACT AGT^ACT
SphI 1289 GCATGC GCATG^C
SspBI 869 TGTACA T^GTACA
SspI 1525 AATATT AAT^ATT
TfiI 650 GATTC G^A[AT]TC
Van91I 1063 CCATTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwNI 251 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
580 CAGTTCCTG
AvaII 673 GGACC G^G[AT]CC
1055 GGTCC
BanII 7 GAGCCC G[GA]GC[CT]^C
836 GAGCCC
BcnI 786 CCCGG CC^[GC]GG
1172 CCCGG
BfaI 1129 CTAG C^TAG
1373 CTAG
BpmI 608 CTCCAG CTGGAG(16/14)
1313 CTGGAG
BsmAI 1486 GAGAC GTCTC(1/5)
606 GTCTC
BstAPI 1159 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1323 GCAAACCCTGC
BstNI 558 CCTGG CC^[AT]GG
705 CCTGG
BstYI 316 GGATCT [GA]^GATC[CT]
1196 AGATCC
EcoRII 558 CCTGG ^CC[AT]GG
705 CCTGG
EcoT14I 11 CCAAGG C^C[AT][AT]GG
1388 CCAAGG
HaeIII 402 GGCC GG^CC
1218 GGCC
HgaI 1263 GACGC GACGC(5/10)
1447 GACGC
HincII 33 GTCGAC GT[CT]^[GA]AC
358 GTTGAC
HindII 33 GTCGAC GT[CT]^[GA]AC
358 GTTGAC
HinfI 650 GATTC G^A[ACGT]TC
729 GACTC
MaeI 1129 CTAG C^TAG
1373 CTAG
MflI 316 GGATCT [GA]^GATC[CT]
1196 AGATCC
MspA1I 1163 CAGCTG C[AC]G^C[GT]G
1299 CAGCGG
MvaI 558 CCTGG CC^[AT]GG
705 CCTGG
NciI 786 CCCGG CC^[GC]GG
1172 CCCGG
NlaIV 860 GGTACC GG[ACGT]^[ACGT]CC
1055 GGTCCC
NspI 866 GCATGT [GA]CATG^[CT]
1289 GCATGC
PstI 956 CTGCAG CTGCA^G
1166 CTGCAG
StyI 11 CCAAGG C^C[AT][AT]GG
1388 CCAAGG
XhoII 316 GGATCT [GA]^GATC[CT]
1196 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI BbvI BmyI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I CfoI Cfr13I DdeI
DpnII EarI EcoRV Fnu4HI FokI
HapII HhaI HinP1I HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MnlI MseI MspI MwoI NdeII
NlaIII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I