Restriction map for
pF1KB9513
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII AccII AclI AcsI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AviII AvrII BbeI BbrPI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BseRI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO109I EcoO65I EcoRI
FbaI FseI FspI HaeII HpaI
KasI KspI MaeI MfeI MluI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacII SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 299 GGTACC G^GTACC
AccI 96 GTCGAC GT^[AC][GT]AC
AcyI 1273 GGCGTC G[GA]^CG[CT]C
Asp718I 299 GGTACC G^GTACC
BalI 833 TGGCCA TGG^CCA
BamHI 415 GGATCC G^GATCC
BbsI 816 GAAGAC GAAGAC(2/6)
BpmI 811 CTGGAG CTGGAG(16/14)
BpuAI 816 GAAGAC GAAGAC(2/6)
BsaBI 544 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1273 GGCGTC G[GA]^CG[CT]C
BsiEI 1071 CGGCCG CG[GA][CT]^CG
BspHI 1131 TCATGA T^CATGA
BstAPI 513 GCAGCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 430 TTCGAA TT^CGAA
BstXI 836 CCAAGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 415 GGATCC [GA]^GATC[CT]
DsaI 288 CCACGG C^C[GA][CT]GG
EagI 1071 CGGCCG C^GGCCG
EarI 1105 GAAGAG CTCTTC(1/4)
EclXI 1071 CGGCCG C^GGCCG
Eco52I 1071 CGGCCG C^GGCCG
EcoRV 544 GATATC GAT^ATC
EcoT22I 1418 ATGCAT ATGCA^T
Hin1I 1273 GGCGTC G[GA]^CG[CT]C
HindIII 389 AAGCTT A^AGCTT
KpnI 299 GGTACC GGTAC^C
Ksp632I 1105 GAAGAG CTCTTC(1/4)
MaeII 792 ACGT A^CGT
MamI 544 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 415 GGATCC [GA]^GATC[CT]
MluNI 833 TGGCCA TGG^CCA
MscI 833 TGGCCA TGG^CCA
MslI 348 CACTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 1418 ATGCAT ATGCA^T
NspV 430 TTCGAA TT^CGAA
RcaI 1131 TCATGA T^CATGA
SalI 96 GTCGAC G^TCGAC
SapI 1105 GAAGAGC GCTCTTC(1/4)
SfuI 430 TTCGAA TT^CGAA
XcmI 667 CCAGATTTTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 415 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccIII 418 TCCGGA T^CCGGA
1256 TCCGGA
AflIII 404 ACATGT A^C[GA][CT]GT
1376 ACATGT
AlwNI 532 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
754 CAGTATCTG
AvaII 626 GGACC G^G[AT]CC
1356 GGTCC
BbvCI 63 CCTCAGC CCTCAGC(-5/-2)
537 GCTGAGG
BcnI 90 CCCGG CC^[GC]GG
741 CCCGG
BsaI 607 GAGACC GGTCTC(1/5)
264 GGTCTC
BsaWI 418 TCCGGA [AT]^CCGG[AT]
1256 TCCGGA
BseAI 418 TCCGGA T^CCGGA
1256 TCCGGA
BsgI 345 CTGCAC GTGCAG(16/14)
511 GTGCAG
BsmFI 1373 GGGAC GGGAC(10/14)
850 GTCCC
BspEI 418 TCCGGA T^CCGGA
1256 TCCGGA
BspLU11I 404 ACATGT A^CATGT
1376 ACATGT
EcoT14I 1163 CCTTGG C^C[AT][AT]GG
1189 CCAAGG
FauI 246 CCCGC CCCGC(4/6)
1341 CCCGC
HgaI 774 GCGTC GACGC(5/10)
1274 GCGTC
HincII 96 GTCGAC GT[CT]^[GA]AC
946 GTTGAC
HindII 96 GTCGAC GT[CT]^[GA]AC
946 GTTGAC
MroI 418 TCCGGA T^CCGGA
1256 TCCGGA
MseI 257 TTAA T^TAA
971 TTAA
MspA1I 532 CAGCTG C[AC]G^C[GT]G
808 CAGCTG
NciI 90 CCCGG CC^[GC]GG
741 CCCGG
PflMI 575 CCAATGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
753 CCAGTATCTGG
PstI 70 CTGCAG CTGCA^G
587 CTGCAG
PvuII 532 CAGCTG CAG^CTG
808 CAGCTG
SacI 490 GAGCTC GAGCT^C
1108 GAGCTC
SfcI 70 CTGCAG C^T[GA][CT]AG
587 CTGCAG
StyI 1163 CCTTGG C^C[AT][AT]GG
1189 CCAAGG
Tru9I 257 TTAA T^TAA
971 TTAA
Van91I 575 CCAATGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
753 CCAGTATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BanI BanII BbvI BmyI BsaJI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I