Restriction map for
pF1KB9512
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII AccI AclI AcsI AcyI
AflII AgeI AhdI AocI Aor51HI
ApoI AscI AspEI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KspI MaeI MamI MfeI
MluI MluNI MscI MunI NarI
NcoI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TthHB8I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 919 GGTACC G^GTACC
AccIII 371 TCCGGA T^CCGGA
AflIII 872 ACGTGT A^C[GA][CT]GT
Alw44I 1134 GTGCAC G^TGCAC
AlwNI 410 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1134 GTGCAC G^TGCAC
AseI 231 ATTAAT AT^TAAT
AsnI 231 ATTAAT AT^TAAT
Asp700I 543 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 919 GGTACC G^GTACC
AspI 1417 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 481 CCTCAGC CCTCAGC(-5/-2)
BcgI 1236 CGAGAAGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 10 GCCGGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 371 TCCGGA T^CCGGA
BsiEI 24 CGGCCG CG[GA][CT]^CG
BspEI 371 TCCGGA T^CCGGA
BstYI 1093 GGATCT [GA]^GATC[CT]
CfoI 68 GCGC GCG^C
DraIII 487 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1033 GACCTGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 24 CGGCCG C^GGCCG
EarI 713 CTCTTC CTCTTC(1/4)
EclXI 24 CGGCCG C^GGCCG
Eco52I 24 CGGCCG C^GGCCG
EcoNI 1296 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1273 GATATC GAT^ATC
HhaI 68 GCGC GCG^C
HinP1I 68 GCGC G^CGC
KpnI 919 GGTACC GGTAC^C
Ksp632I 713 CTCTTC CTCTTC(1/4)
MflI 1093 GGATCT [GA]^GATC[CT]
MroI 371 TCCGGA T^CCGGA
MslI 964 CACAGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 1280 CATATG CA^TATG
PflFI 1417 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 194 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 231 ATTAAT AT^TAAT
PstI 100 CTGCAG CTGCA^G
SacI 565 GAGCTC GAGCT^C
SmlI 668 CTCAAG C^T[CT][GA]AG
Tsp509I 230 AATT ^AATT
Tth111I 1417 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 194 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1093 GGATCT [GA]^GATC[CT]
XmnI 543 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 920 GTAC GT^AC
1482 GTAC
AlwI 180 GATCC GGATC(4/5)
1093 GGATC
ApaI 190 GGGCCC GGGCC^C
459 GGGCCC
AspHI 565 GAGCTC G[AT]GC[AT]^C
1134 GTGCAC
BanI 349 GGCACC G^G[CT][GA]CC
919 GGTACC
BpmI 140 CTGGAG CTGGAG(16/14)
404 CTGGAG
BsaI 739 GAGACC GGTCTC(1/5)
1041 GGTCTC
BsaWI 371 TCCGGA [AT]^CCGG[AT]
1433 ACCGGA
BsgI 157 CTGCAC GTGCAG(16/14)
162 CTGCAC
BsiHKAI 565 GAGCTC G[AT]GC[AT]^C
1134 GTGCAC
BsmI 1242 GAATGC GAATGC(1/-1)
1361 GCATTC
DpnII 180 GATC ^GATC
1094 GATC
DsaI 449 CCGTGG C^C[GA][CT]GG
513 CCACGG
EaeI 24 CGGCCG [CT]^GGCC[GA]
149 CGGCCA
MboI 180 GATC ^GATC
1094 GATC
MseI 232 TTAA T^TAA
398 TTAA
NdeII 180 GATC ^GATC
1094 GATC
PpuMI 707 GGGACCC [GA]G^G[AT]CC[CT]
1496 AGGTCCC
PvuII 137 CAGCTG CAG^CTG
847 CAGCTG
RsaI 920 GTAC GT^AC
1482 GTAC
Sau3AI 180 GATC ^GATC
1094 GATC
SexAI 797 ACCAGGT A^CC[AT]GGT
853 ACCTGGT
SmaI 224 CCCGGG CCC^GGG
263 CCCGGG
TfiI 108 GATTC G^A[AT]TC
1181 GAATC
Tru9I 232 TTAA T^TAA
398 TTAA
XmaCI 224 CCCGGG C^CCGGG
263 CCCGGG
XmaI 224 CCCGGG C^CCGGG
263 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AluI AvaI AvaII
BanII BbvI BcnI BmyI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
Cfr10I Cfr13I DdeI DraII EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
HphI ItaI MaeII MaeIII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NgoMIV NlaIII
NlaIV PleI Sau96I ScrFI SfaNI
SfcI StyI TseI Tsp45I TspRI