Restriction map for
pF1KB9509
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BciVI BclI BglII
BlnI BlpI BmyI Bpu1102I BsaAI
BsaHI BsaI BsaJI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspEI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII CpoI
DraI DraIII DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I FbaI
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI KspI MaeIII
MfeI MluI MluNI MroI MscI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tsp45I Tth111I Van91I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AcsI 321 AAATTT [GA]^AATT[CT]
AflII 543 CTTAAG C^TTAAG
ApoI 321 AAATTT [GA]^AATT[CT]
BcgI 107 GCAGTGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 594 CCGGG CC^[GC]GG
BfrI 543 CTTAAG C^TTAAG
BglI 779 GCCACAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 244 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 970 GAGAC GTCTC(1/5)
BspDI 1057 ATCGAT AT^CGAT
BspMI 758 ACCTGC ACCTGC(4/8)
BsrDI 840 CATTGC GCAATG(2/0)
BsrI 1011 CCAGT ACTGG(1/-1)
Cac8I 1032 GCTCGC GC[ACGT]^[ACGT]GC
ClaI 1057 ATCGAT AT^CGAT
DrdI 55 GACAGGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 119 TGGCCG [CT]^GGCC[GA]
EarI 505 CTCTTC CTCTTC(1/4)
EcoT22I 333 ATGCAT ATGCA^T
HaeII 417 GGCGCT [GA]GCGC^[CT]
Ksp632I 505 CTCTTC CTCTTC(1/4)
MaeII 1066 ACGT A^CGT
MamI 244 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 103 CACAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 594 CCGGG CC^[GC]GG
NsiI 333 ATGCAT ATGCA^T
PacI 325 TTAATTAA TTAAT^TAA
PstI 742 CTGCAG CTGCA^G
SexAI 749 ACCTGGT A^CC[AT]GGT
XbaI 438 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccII 13 CGCG CG^CG
18 CGCG
AlwI 375 GGATC GGATC(4/5)
580 GGATC
AvaII 171 GGACC G^G[AT]CC
1005 GGACC
BbsI 300 GAAGAC GAAGAC(2/6)
362 GTCTTC
BfaI 439 CTAG C^TAG
571 CTAG
BpuAI 300 GAAGAC GAAGAC(2/6)
362 GTCTTC
BsrBI 157 GAGCGG CCGCTC(-3/-3)
985 GAGCGG
BsrFI 719 ACCGGC [GA]^CCGG[CT]
1016 ACCGGC
BstUI 13 CGCG CG^CG
18 CGCG
BstXI 212 CCATCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
959 CCAGGAGCCTGG
BstYI 375 GGATCT [GA]^GATC[CT]
580 GGATCT
Cfr10I 719 ACCGGC [GA]^CCGG[CT]
1016 ACCGGC
HaeIII 120 GGCC GG^CC
805 GGCC
HphI 753 GGTGA GGTGA(8/7)
686 TCACC
MaeI 439 CTAG C^TAG
571 CTAG
MflI 375 GGATCT [GA]^GATC[CT]
580 GGATCT
MvnI 13 CGCG CG^CG
18 CGCG
PpuMI 170 AGGACCC [GA]G^G[AT]CC[CT]
1004 AGGACCC
SmlI 543 CTTAAG C^T[CT][GA]AG
922 CTTGAG
XhoII 375 GGATCT [GA]^GATC[CT]
580 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AlwNI BbvI
BpmI BseRI BsiYI BslI BssKI
BstF5I BstNI CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII FauI
Fnu4HI FokI HapII HhaI HinP1I
HincII HindII HinfI HpaII ItaI
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I