Restriction map for
pF1KB9503
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# NO CUT ENZYMES ( 98 enzymes) #
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Acc65I AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AvrII BciVI BfrI BglI
BlnI BlpI Bpu1102I BseAI BsiEI
BsiWI Bsp1407I BspEI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstPI
Bsu36I CelII Cfr10I CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FseI HindIII HpaI KpnI
MfeI MluI MroI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
PvuII RsrII SacI SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AatII 3391 GACGTC GACGT^C
AvaI 2699 CTCGGG C^[CT]CG[GA]G
AviII 1406 TGCGCA TGC^GCA
BbeI 2551 GGCGCC GGCGC^C
BbrPI 2276 CACGTG CAC^GTG
BclI 2318 TGATCA T^GATCA
BglII 1725 AGATCT A^GATCT
BsaAI 2276 CACGTG [CT]AC^GT[GA]
BsmBI 1095 CGTCTC CGTCTC(1/5)
BsoBI 2699 CTCGGG C^[CT]CG[GA]G
BspDI 2654 ATCGAT AT^CGAT
BspHI 407 TCATGA T^CATGA
BspLU11I 3018 ACATGT A^CATGT
BspMI 3621 GCAGGT ACCTGC(4/8)
Bst1107I 3625 GTATAC GTA^TAC
BstZ17I 3625 GTATAC GTA^TAC
ClaI 2654 ATCGAT AT^CGAT
EcoNI 3664 CCTTCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3466 GAATTC G^AATTC
FbaI 2318 TGATCA T^GATCA
FspI 1406 TGCGCA TGC^GCA
KasI 2551 GGCGCC G^GCGCC
KspI 3435 CCGCGG CCGC^GG
NarI 2551 GGCGCC GG^CGCC
PmaCI 2276 CACGTG CAC^GTG
PmlI 2276 CACGTG CAC^GTG
PshAI 1062 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 407 TCATGA T^CATGA
SacII 3435 CCGCGG CCGC^GG
SfoI 2551 GGCGCC GGC^GCC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccI 3175 GTAGAC GT^[AC][GT]AC
3625 GTATAC
AflIII 2277 ACGTGT A^C[GA][CT]GT
3018 ACATGT
AspHI 335 GTGCTC G[AT]GC[AT]^C
2273 GAGCAC
BamHI 1586 GGATCC G^GATCC
2981 GGATCC
BbvCI 452 CCTCAGC CCTCAGC(-5/-2)
2296 GCTGAGG
BcgI 1897 CGAGCCAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2691 GCAGATTACTCG
BsaI 1873 GAGACC GGTCTC(1/5)
1971 GGTCTC
BsgI 1990 CTGCAC GTGCAG(16/14)
1468 GTGCAG
BsiHKAI 335 GTGCTC G[AT]GC[AT]^C
2273 GAGCAC
BsmI 694 GCATTC GAATGC(1/-1)
2585 GCATTC
BsrDI 891 CATTGC GCAATG(2/0)
950 CATTGC
BstAPI 457 GCAACTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2142 GCAGCTCCTGC
DsaI 2072 CCGTGG C^C[GA][CT]GG
3435 CCGCGG
HaeII 2551 GGCGCC [GA]GCGC^[CT]
3615 GGCGCT
HincII 1059 GTTGAC GT[CT]^[GA]AC
2188 GTTGAC
HindII 1059 GTTGAC GT[CT]^[GA]AC
2188 GTTGAC
NspI 1706 ACATGC [GA]CATG^[CT]
3018 ACATGT
PflMI 2382 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2512 CCACGCTCTGG
PleI 444 GACTC GAGTC(4/5)
2407 GACTC
SapI 1356 GAAGAGC GCTCTTC(1/4)
1428 GAAGAGC
Van91I 2382 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2512 CCACGCTCTGG
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# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
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AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaBI BsaHI BsaJI
BsaWI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflFI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XcmI XhoII