Restriction map for
pF1KB9501
##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AvaI BbrPI BbvCI BciVI BfrI
BlpI Bpu1102I BsaAI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BsrBI BssHII Bst1107I BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DraIII DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoRI EcoT22I FauI FseI HgaI
KpnI KspI MluI MroI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcyI 2135 GGCGCC G[GA]^CG[CT]C
AflIII 2894 ACATGT A^C[GA][CT]GT
AhdI 3308 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 397 GTGCAC G^TGCAC
ApaLI 397 GTGCAC G^TGCAC
Asp700I 172 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3308 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2101 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 653 TGCGCA TGC^GCA
AvrII 1875 CCTAGG C^CTAGG
BalI 227 TGGCCA TGG^CCA
BamHI 1373 GGATCC G^GATCC
BbeI 2135 GGCGCC GGCGC^C
BcgI 2817 GCATTATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2043 GCCCTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1875 CCTAGG C^CTAGG
BsaHI 2135 GGCGCC G[GA]^CG[CT]C
Bsp1407I 287 TGTACA T^GTACA
BspHI 1832 TCATGA T^CATGA
BspLU11I 2894 ACATGT A^CATGT
BsrDI 3341 CATTGC GCAATG(2/0)
BsrFI 898 GCCGGT [GA]^CCGG[CT]
BsrGI 287 TGTACA T^GTACA
BssSI 2353 CTCGTG CACGAG(-5/-1)
Cfr10I 898 GCCGGT [GA]^CCGG[CT]
DrdI 2113 GACACGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3308 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 142 GATATC GAT^ATC
FspI 653 TGCGCA TGC^GCA
HaeII 2135 GGCGCC [GA]GCGC^[CT]
Hin1I 2135 GGCGCC G[GA]^CG[CT]C
HindIII 1546 AAGCTT A^AGCTT
HpaI 3028 GTTAAC GTT^AAC
KasI 2135 GGCGCC G^GCGCC
MfeI 2636 CAATTG C^AATTG
MluNI 227 TGGCCA TGG^CCA
MscI 227 TGGCCA TGG^CCA
MunI 2636 CAATTG C^AATTG
NarI 2135 GGCGCC GG^CGCC
PflFI 2101 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 30 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1832 TCATGA T^CATGA
SacI 922 GAGCTC GAGCT^C
ScaI 2333 AGTACT AGT^ACT
SfoI 2135 GGCGCC GGC^GCC
SspBI 287 TGTACA T^GTACA
StuI 2050 AGGCCT AGG^CCT
Tth111I 2101 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 172 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 570 CGCG CG^CG
625 CGCG
AspHI 397 GTGCAC G[AT]GC[AT]^C
922 GAGCTC
BbsI 2983 GAAGAC GAAGAC(2/6)
3316 GTCTTC
BclI 1121 TGATCA T^GATCA
1242 TGATCA
BfaI 1876 CTAG C^TAG
2182 CTAG
BpuAI 2983 GAAGAC GAAGAC(2/6)
3316 GTCTTC
BsaI 2626 GAGACC GGTCTC(1/5)
496 GGTCTC
BsiHKAI 397 GTGCAC G[AT]GC[AT]^C
922 GAGCTC
BsmFI 412 GGGAC GGGAC(10/14)
3244 GGGAC
BsmI 72 GAATGC GAATGC(1/-1)
3259 GAATGC
BspMI 620 ACCTGC ACCTGC(4/8)
2161 GCAGGT
BstEII 351 GGTCACC G^GT[ACGT]ACC
951 GGTGACC
BstPI 351 GGTCACC G^GT[ACGT]ACC
951 GGTGACC
BstUI 570 CGCG CG^CG
625 CGCG
BstXI 956 CCAAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1303 CCAGGAAAATGG
EaeI 227 TGGCCA [CT]^GGCC[GA]
1632 TGGCCG
EcoO65I 351 GGTCACC G^GT[ACGT]ACC
951 GGTGACC
EcoT14I 1875 CCTAGG C^C[AT][AT]GG
3240 CCTTGG
FbaI 1121 TGATCA T^GATCA
1242 TGATCA
MaeI 1876 CTAG C^TAG
2182 CTAG
MaeII 507 ACGT A^CGT
548 ACGT
MslI 2149 CACATTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2349 CATTCTCGTG
MvnI 570 CGCG CG^CG
625 CGCG
PstI 1151 CTGCAG CTGCA^G
3069 CTGCAG
SfcI 1151 CTGCAG C^T[GA][CT]AG
3069 CTGCAG
SphI 629 GCATGC GCATG^C
650 GCATGC
SspI 1157 AATATT AAT^ATT
2867 AATATT
StyI 1875 CCTAGG C^C[AT][AT]GG
3240 CCTTGG
XcmI 2473 CCAGAATTAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2840 CCATTACTGAATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BanII BbvI
BcnI BglII BmyI BpmI BsaBI
BsaJI BseRI BsgI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoNI
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeIII
MamI MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII