Restriction map for
pF1KB9500
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII AccI AclI AcyI AflIII
AgeI AlwNI AocI Aor51HI ApaI
AscI AvaI AviII AvrII BamHI
BbeI BbrPI BciVI BglI BlnI
BsaAI BsaBI BsaHI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspLU11I BspMI BsrBI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CfoI ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV FauI FseI
FspI HaeII HhaI Hin1I HinP1I
HpaI KasI KspI MamI MluI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NspV PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 2004 GGTACC G^GTACC
AccII 1595 CGCG CG^CG
AccIII 1754 TCCGGA T^CCGGA
AflII 259 CTTAAG C^TTAAG
AhdI 955 GACTTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 158 GTGCAC G^TGCAC
AlwI 1156 GATCC GGATC(4/5)
ApaLI 158 GTGCAC G^TGCAC
AseI 910 ATTAAT AT^TAAT
AsnI 910 ATTAAT AT^TAAT
Asp700I 1807 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2004 GGTACC G^GTACC
AspEI 955 GACTTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 158 GTGCAC G[AT]GC[AT]^C
AspI 826 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 703 GAGCCC G[GA]GC[CT]^C
BbvCI 2019 GCTGAGG CCTCAGC(-5/-2)
BcgI 672 CGATAAGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 259 CTTAAG C^TTAAG
BlpI 682 GCTGAGC GC^T[ACGT]AGC
BpmI 119 CTGGAG CTGGAG(16/14)
Bpu1102I 682 GCTGAGC GC^T[ACGT]AGC
BseAI 1754 TCCGGA T^CCGGA
BsiHKAI 158 GTGCAC G[AT]GC[AT]^C
BsmFI 1187 GGGAC GGGAC(10/14)
BspEI 1754 TCCGGA T^CCGGA
BspHI 1496 TCATGA T^CATGA
BsrDI 1788 CATTGC GCAATG(2/0)
BsrFI 1570 GCCGGT [GA]^CCGG[CT]
BssSI 785 CTCGTG CACGAG(-5/-1)
BstAPI 687 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1595 CGCG CG^CG
CelII 682 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1570 GCCGGT [GA]^CCGG[CT]
Eam1105I 955 GACTTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 1028 CCAAGG C^C[AT][AT]GG
HgaI 1485 GCGTC GACGC(5/10)
HindIII 1230 AAGCTT A^AGCTT
KpnI 2004 GGTACC GGTAC^C
MaeII 150 ACGT A^CGT
MfeI 1925 CAATTG C^AATTG
MroI 1754 TCCGGA T^CCGGA
MspA1I 1622 CAGCTG C[AC]G^C[GT]G
MunI 1925 CAATTG C^AATTG
MvnI 1595 CGCG CG^CG
NdeI 13 CATATG CA^TATG
NspI 24 GCATGT [GA]CATG^[CT]
PacI 1972 TTAATTAA TTAAT^TAA
PflFI 826 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 984 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 910 ATTAAT AT^TAAT
PvuII 1622 CAGCTG CAG^CTG
RcaI 1496 TCATGA T^CATGA
SspI 360 AATATT AAT^ATT
StuI 1172 AGGCCT AGG^CCT
StyI 1028 CCAAGG C^C[AT][AT]GG
Tsp45I 1407 GTCAC ^GT[GC]AC
Tth111I 826 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 903 TCTAGA T^CTAGA
XmnI 1807 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AvaII 1456 GGTCC G^G[AT]CC
1512 GGACC
BanI 130 GGCACC G^G[CT][GA]CC
2004 GGTACC
BclI 1142 TGATCA T^GATCA
1697 TGATCA
BcnI 1211 CCGGG CC^[GC]GG
1544 CCCGG
BglII 561 AGATCT A^GATCT
1385 AGATCT
BsaI 1437 GAGACC GGTCTC(1/5)
1556 GAGACC
BsaWI 289 ACCGGA [AT]^CCGG[AT]
1754 TCCGGA
BseRI 749 GAGGAG GAGGAG(10/8)
1381 GAGGAG
BsmI 480 GAATGC GAATGC(1/-1)
1603 GCATTC
EcoRI 1758 GAATTC G^AATTC
1932 GAATTC
EcoT22I 482 ATGCAT ATGCA^T
970 ATGCAT
FbaI 1142 TGATCA T^GATCA
1697 TGATCA
HincII 109 GTTGAC GT[CT]^[GA]AC
1004 GTCAAC
HindII 109 GTTGAC GT[CT]^[GA]AC
1004 GTCAAC
MaeIII 28 GTTAC ^GT[ACGT]AC
1407 GTCAC
MslI 13 CATATGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
132 CACCAGGATG
NciI 1211 CCGGG CC^[GC]GG
1544 CCCGG
NsiI 482 ATGCAT ATGCA^T
970 ATGCAT
PleI 826 GACTC GAGTC(4/5)
1749 GACTC
PstI 863 CTGCAG CTGCA^G
1526 CTGCAG
SmlI 259 CTTAAG C^T[CT][GA]AG
569 CTCAAG
TspRI 443 CACTG CA[GC]TG[ACGT][ACGT]^
1999 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BalI BbsI BbvI BfaI BmyI
BpuAI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII EaeI EarI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MboI
MboII MflI MluNI MnlI MscI
MseI MspI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp509I TthHB8I XhoII