Restriction map for
pF1KB9498
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BcgI
BciVI BfrI BglI BlnI BlpI
Bpu1102I BseAI BsiWI Bsp1407I BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT14I EcoT22I FseI
FspI HindIII HpaI KpnI KspI
MluI MluNI MroI MscI MslI
MvnI NaeI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PmeI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfaNI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tsp45I Tth111I Van91I XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcyI 192 GGCGCC G[GA]^CG[CT]C
AgeI 692 ACCGGT A^CCGGT
Asp700I 340 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 192 GGCGCC G^G[CT][GA]CC
BbeI 192 GGCGCC GGCGC^C
BbrPI 380 CACGTG CAC^GTG
BglII 1711 AGATCT A^GATCT
BsaAI 380 CACGTG [CT]AC^GT[GA]
BsaHI 192 GGCGCC G[GA]^CG[CT]C
BsaI 1658 GAGACC GGTCTC(1/5)
BseRI 875 GAGGAG GAGGAG(10/8)
BsmBI 1643 GAGACG CGTCTC(1/5)
BspDI 749 ATCGAT AT^CGAT
BsrFI 692 ACCGGT [GA]^CCGG[CT]
BstXI 109 CCAGTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1711 AGATCT [GA]^GATC[CT]
CfoI 193 GCGC GCG^C
Cfr10I 692 ACCGGT [GA]^CCGG[CT]
ClaI 749 ATCGAT AT^CGAT
EcoNI 637 CCTAGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 881 GCGGG CCCGC(4/6)
HaeII 192 GGCGCC [GA]GCGC^[CT]
HgaI 1164 GCGTC GACGC(5/10)
HhaI 193 GCGC GCG^C
Hin1I 192 GGCGCC G[GA]^CG[CT]C
HinP1I 193 GCGC G^CGC
HincII 1099 GTTGAC GT[CT]^[GA]AC
HindII 1099 GTTGAC GT[CT]^[GA]AC
KasI 192 GGCGCC G^GCGCC
MfeI 200 CAATTG C^AATTG
MflI 1711 AGATCT [GA]^GATC[CT]
MunI 200 CAATTG C^AATTG
NarI 192 GGCGCC GG^CGCC
PaeR7I 1064 CTCGAG C^TCGAG
PinAI 692 ACCGGT A^CCGGT
PmaCI 380 CACGTG CAC^GTG
PmlI 380 CACGTG CAC^GTG
SapI 833 GAAGAGC GCTCTTC(1/4)
ScaI 1018 AGTACT AGT^ACT
SfoI 192 GGCGCC GGC^GCC
SmlI 1064 CTCGAG C^T[CT][GA]AG
XbaI 1335 TCTAGA T^CTAGA
XhoI 1064 CTCGAG C^TCGAG
XhoII 1711 AGATCT [GA]^GATC[CT]
XmnI 340 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AocI 1024 CCTAAGG CC^T[ACGT]AGG
1735 CCTGAGG
AspHI 930 GAGCTC G[AT]GC[AT]^C
1488 GAGCTC
BbvCI 8 CCTCAGC CCTCAGC(-5/-2)
64 GCTGAGG
BclI 513 TGATCA T^GATCA
1401 TGATCA
BsaWI 257 ACCGGA [AT]^CCGG[AT]
692 ACCGGT
BsgI 1715 CTGCAC GTGCAG(16/14)
365 GTGCAG
BsiHKAI 930 GAGCTC G[AT]GC[AT]^C
1488 GAGCTC
BsmI 1443 GAATGC GAATGC(1/-1)
1599 GAATGC
Bsu36I 1024 CCTAAGG CC^T[ACGT]AGG
1735 CCTGAGG
DsaI 229 CCACGG C^C[GA][CT]GG
1632 CCGTGG
EaeI 552 TGGCCG [CT]^GGCC[GA]
759 TGGCCG
Eco81I 1024 CCTAAGG CC^T[ACGT]AGG
1735 CCTGAGG
EcoRI 340 GAATTC G^AATTC
494 GAATTC
FbaI 513 TGATCA T^GATCA
1401 TGATCA
MaeII 381 ACGT A^CGT
506 ACGT
MseI 335 TTAA T^TAA
355 TTAA
SacI 930 GAGCTC GAGCT^C
1488 GAGCTC
Tru9I 335 TTAA T^TAA
355 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaBI BsaJI BsiEI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MamI MboI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIV PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfcI TaqI TfiI TseI
Tsp509I TspRI TthHB8I