Restriction map for
pF1KB9495
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BbrPI BbvCI BclI BlnI
BsaI BsaWI BseAI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII EcoO65I EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI HphI KpnI MluI MroI
NcoI NdeI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI SgrAI SnaBI SpeI
SphI SspI SwaI Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 1578 GTCTAC GT^[AC][GT]AC
AcsI 1179 GAATTC [GA]^AATT[CT]
AflII 1174 CTTAAG C^TTAAG
Aor51HI 1432 AGCGCT AGC^GCT
ApoI 1179 GAATTC [GA]^AATT[CT]
BamHI 508 GGATCC G^GATCC
BbsI 673 GAAGAC GAAGAC(2/6)
BcgI 282 GCAGCGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1174 CTTAAG C^TTAAG
BglII 633 AGATCT A^GATCT
BlpI 1435 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1435 GCTCAGC GC^T[ACGT]AGC
BpuAI 673 GAAGAC GAAGAC(2/6)
BsaAI 2155 TACGTG [CT]AC^GT[GA]
BsgI 832 GTGCAG GTGCAG(16/14)
Bsp1407I 1506 TGTACA T^GTACA
BsrDI 738 CATTGC GCAATG(2/0)
BsrGI 1506 TGTACA T^GTACA
BstBI 1182 TTCGAA TT^CGAA
BstXI 2207 CCACCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1435 GCTCAGC GC^T[ACGT]AGC
DrdI 1410 GACAGGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1432 AGCGCT AGC^GCT
EcoRI 1179 GAATTC G^AATTC
MaeII 2156 ACGT A^CGT
MfeI 340 CAATTG C^AATTG
MunI 340 CAATTG C^AATTG
NaeI 48 GCCGGC GCC^GGC
NgoMIV 48 GCCGGC G^CCGGC
NheI 2161 GCTAGC G^CTAGC
NspI 1386 ACATGC [GA]CATG^[CT]
NspV 1182 TTCGAA TT^CGAA
SbfI 1542 CCTGCAGG CCTGCA^GG
ScaI 2174 AGTACT AGT^ACT
SexAI 1658 ACCTGGT A^CC[AT]GGT
SfiI 147 GGCCACGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1182 TTCGAA TT^CGAA
Sse8387I 1542 CCTGCAGG CCTGCA^GG
SspBI 1506 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
ApaI 1909 GGGCCC GGGCC^C
2078 GGGCCC
AspHI 445 GAGCAC G[AT]GC[AT]^C
2307 GAGCAC
BalI 1974 TGGCCA TGG^CCA
1997 TGGCCA
BciVI 1211 GGATAC GTATCC(6/5)
1854 GGATAC
BsaBI 745 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
793 GATGTATATC
BsiHKAI 445 GAGCAC G[AT]GC[AT]^C
2307 GAGCAC
BsrBI 118 GAGCGG CCGCTC(-3/-3)
136 GAGCGG
BsrFI 48 GCCGGC [GA]^CCGG[CT]
2027 ACCGGC
Cfr10I 48 GCCGGC [GA]^CCGG[CT]
2027 ACCGGC
HgaI 1550 GCGTC GACGC(5/10)
1730 GCGTC
KspI 1758 CCGCGG CCGC^GG
1937 CCGCGG
MamI 745 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
793 GATGTATATC
MluNI 1974 TGGCCA TGG^CCA
1997 TGGCCA
MscI 1974 TGGCCA TGG^CCA
1997 TGGCCA
MslI 901 CATGCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2208 CACCGTGGTG
PflMI 1638 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1773 CCAGGGGCTGG
PstI 629 CTGCAG CTGCA^G
1543 CTGCAG
SacII 1758 CCGCGG CCGC^GG
1937 CCGCGG
SapI 442 GAAGAGC GCTCTTC(1/4)
1631 GCTCTTC
SfaNI 364 GATGC GCATC(5/9)
934 GATGC
Van91I 1638 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1773 CCAGGGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI AocI AspEI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BfaI BglI BmyI
BpmI BsaHI BsaJI BseRI BsiEI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII ItaI KasI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfcI SfoI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI