Restriction map for
pF1KB9493
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmBI
BsmFI BsmI Bsp1286I Bsp1407I BspDI
BspEI BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HapII HgaI
Hin1I HincII HindII HindIII HpaI
HpaII HphI KasI KpnI KspI
MaeIII MamI MflI MluI MluNI
MroI MscI MspA1I MspI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 548 CGCG CG^CG
AcsI 133 GAATTC [GA]^AATT[CT]
AflIII 165 ACATGT A^C[GA][CT]GT
ApoI 133 GAATTC [GA]^AATT[CT]
AvaI 272 CTCGAG C^[CT]CG[GA]G
BfaI 10 CTAG C^TAG
BpmI 28 CTGGAG CTGGAG(16/14)
BsaJI 306 CCTTGG C^C[ACGT][ACGT]GG
BseRI 418 CTCCTC GAGGAG(10/8)
BsmAI 175 GAGAC GTCTC(1/5)
BsoBI 272 CTCGAG C^[CT]CG[GA]G
BspHI 456 TCATGA T^CATGA
BspLU11I 165 ACATGT A^CATGT
BsrDI 140 GCAATG GCAATG(2/0)
BsrI 251 CCAGT ACTGG(1/-1)
BssKI 370 CCAGG ^CC[ACGT]GG
BstAPI 241 GCATTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 268 CATCC GGATG(2/0)
BstNI 370 CCAGG CC^[AT]GG
BstUI 548 CGCG CG^CG
Cac8I 578 GCAGGC GC[ACGT]^[ACGT]GC
DdeI 531 CTTAG C^T[ACGT]AG
EarI 298 GAAGAG CTCTTC(1/4)
EcoRI 133 GAATTC G^AATTC
EcoRII 370 CCAGG ^CC[AT]GG
EcoT14I 306 CCTTGG C^C[AT][AT]GG
FokI 268 CATCC GGATG(9/13)
HaeII 6 GGCGCT [GA]GCGC^[CT]
Ksp632I 298 GAAGAG CTCTTC(1/4)
MaeI 10 CTAG C^TAG
MaeII 405 ACGT A^CGT
MfeI 50 CAATTG C^AATTG
MslI 166 CATGTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 50 CAATTG C^AATTG
MvaI 370 CCAGG CC^[AT]GG
MvnI 548 CGCG CG^CG
NspI 165 ACATGT [GA]CATG^[CT]
PaeR7I 272 CTCGAG C^TCGAG
RcaI 456 TCATGA T^CATGA
ScrFI 370 CCAGG CC^[ACGT]GG
SfcI 467 CTGTAG C^T[GA][CT]AG
SmlI 272 CTCGAG C^T[CT][GA]AG
SspI 316 AATATT AAT^ATT
StyI 306 CCTTGG C^C[AT][AT]GG
TaqI 273 TCGA T^CGA
TfiI 311 GATTC G^A[AT]TC
TspRI 252 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 273 TCGA T^CGA
XhoI 272 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AfaI 88 GTAC GT^AC
511 GTAC
AluI 284 AGCT AG^CT
465 AGCT
BbvI 238 GCTGC GCAGC(8/12)
247 GCTGC
CfoI 7 GCGC GCG^C
549 GCGC
Cfr13I 303 GGGCC G^G[ACGT]CC
396 GGGCC
DpnII 412 GATC ^GATC
566 GATC
DraII 302 AGGGCCT [GA]G^G[ACGT]CC[CT]
395 GGGGCCT
EcoO109I 302 AGGGCCT [GA]G^G[ACGT]CC[CT]
395 GGGGCCT
HaeIII 304 GGCC GG^CC
397 GGCC
HhaI 7 GCGC GCG^C
549 GCGC
HinP1I 7 GCGC G^CGC
549 GCGC
MboI 412 GATC ^GATC
566 GATC
MboII 298 GAAGA GAAGA(8/7)
535 GAAGA
MseI 428 TTAA T^TAA
500 TTAA
NdeII 412 GATC ^GATC
566 GATC
NlaIV 15 GGAACC GG[ACGT]^[ACGT]CC
395 GGGGCC
PleI 119 GAGTC GAGTC(4/5)
544 GAGTC
RsaI 88 GTAC GT^AC
511 GTAC
Sau3AI 412 GATC ^GATC
566 GATC
Sau96I 303 GGGCC G^G[ACGT]CC
396 GGGCC
Tru9I 428 TTAA T^TAA
500 TTAA
TseI 238 GCTGC G^C[AT]GC
247 GCTGC
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
#########################################
AciI Fnu4HI HinfI ItaI MnlI
MwoI NlaIII Tsp509I