Restriction map for
pF1KB9492
##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflIII AgeI AhdI AocI Aor51HI
ApaI AscI Asp718I AspEI AspI
AvaI AviII AvrII BanI BbeI
BbrPI BclI BglI BlnI BsaAI
BsaHI BsiEI BsiWI BsmBI BsoBI
BspDI BspHI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I FauI FbaI FseI FspI
HgaI Hin1I HpaI KasI KpnI
KspI MluI MvnI NaeI NarI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI Tth111I Van91I XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 1460 GTCTAC GT^[AC][GT]AC
AccIII 2780 TCCGGA T^CCGGA
AflII 157 CTTAAG C^TTAAG
Alw44I 2024 GTGCAC G^TGCAC
ApaLI 2024 GTGCAC G^TGCAC
AseI 846 ATTAAT AT^TAAT
AsnI 846 ATTAAT AT^TAAT
AspHI 2024 GTGCAC G[AT]GC[AT]^C
BamHI 1035 GGATCC G^GATCC
BbvCI 2167 CCTCAGC CCTCAGC(-5/-2)
BcgI 2891 GCATGTGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1803 GTATCC GTATCC(6/5)
BcnI 1677 CCGGG CC^[GC]GG
BfrI 157 CTTAAG C^TTAAG
BglII 3006 AGATCT A^GATCT
BsaBI 2392 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1786 GAGACC GGTCTC(1/5)
BsaWI 2780 TCCGGA [AT]^CCGG[AT]
BseAI 2780 TCCGGA T^CCGGA
BsiHKAI 2024 GTGCAC G[AT]GC[AT]^C
BspEI 2780 TCCGGA T^CCGGA
BstAPI 1495 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 2001 GCGC GCG^C
EcoNI 1210 CCTTTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2209 GATATC GAT^ATC
HaeII 2000 AGCGCC [GA]GCGC^[CT]
HhaI 2001 GCGC GCG^C
HinP1I 2001 GCGC G^CGC
HincII 97 GTTGAC GT[CT]^[GA]AC
HindII 97 GTTGAC GT[CT]^[GA]AC
HindIII 604 AAGCTT A^AGCTT
MamI 2392 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2780 TCCGGA T^CCGGA
NciI 1677 CCGGG CC^[GC]GG
NdeI 2996 CATATG CA^TATG
NheI 2124 GCTAGC G^CTAGC
PpuMI 3124 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 856 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 846 ATTAAT AT^TAAT
SapI 1368 GAAGAGC GCTCTTC(1/4)
SexAI 3398 ACCAGGT A^CC[AT]GGT
StuI 1218 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwNI 3475 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
3586 CAGAGACTG
Asp700I 271 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1409 GAATATATTC
BalI 1907 TGGCCA TGG^CCA
3275 TGGCCA
BanII 152 GAGCCC G[GA]GC[CT]^C
1580 GAGCCC
BlpI 490 GCTCAGC GC^T[ACGT]AGC
3143 GCTTAGC
Bpu1102I 490 GCTCAGC GC^T[ACGT]AGC
3143 GCTTAGC
BsgI 2840 CTGCAC GTGCAG(16/14)
2687 GTGCAG
Bsp1407I 299 TGTACA T^GTACA
1974 TGTACA
BspMI 3287 ACCTGC ACCTGC(4/8)
3441 GCAGGT
BsrGI 299 TGTACA T^GTACA
1974 TGTACA
BstXI 613 CCACCGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3372 CCAGGATTATGG
CelII 490 GCTCAGC GC^T[ACGT]AGC
3143 GCTTAGC
DraI 1213 TTTAAA TTT^AAA
3231 TTTAAA
DraII 3124 GGGTCCT [GA]G^G[ACGT]CC[CT]
3503 GGGGCCT
DsaI 1098 CCATGG C^C[GA][CT]GG
2609 CCATGG
EaeI 1907 TGGCCA [CT]^GGCC[GA]
3275 TGGCCA
EcoO109I 3124 GGGTCCT [GA]G^G[ACGT]CC[CT]
3503 GGGGCCT
HapII 1677 CCGG C^CGG
2781 CCGG
HpaII 1677 CCGG C^CGG
2781 CCGG
MaeII 25 ACGT A^CGT
2582 ACGT
MfeI 1933 CAATTG C^AATTG
2941 CAATTG
MluNI 1907 TGGCCA TGG^CCA
3275 TGGCCA
MscI 1907 TGGCCA TGG^CCA
3275 TGGCCA
MspI 1677 CCGG C^CGG
2781 CCGG
MunI 1933 CAATTG C^AATTG
2941 CAATTG
NcoI 1098 CCATGG C^CATGG
2609 CCATGG
PleI 453 GAGTC GAGTC(4/5)
2058 GAGTC
SspBI 299 TGTACA T^GTACA
1974 TGTACA
XbaI 265 TCTAGA T^CTAGA
909 TCTAGA
XmnI 271 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1409 GAATATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRI EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NsiI NspI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII