Restriction map for
pF1KB9490
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# NO CUT ENZYMES ( 95 enzymes) #
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AatII AccI AccII AclI AcyI
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI AvrII
BbeI BbrPI BclI BglI BlnI
BsaAI BsaHI BsaI BsiEI BsiWI
BsmAI BsmBI Bsp1407I BspDI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstUI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoT22I
FauI FbaI FseI HaeII Hin1I
HincII HindII HpaI KasI KspI
MluI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SgrAI
SmaI SnaBI Sse8387I SspBI SspI
StuI SwaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 128 GGTACC G^GTACC
AccIII 3116 TCCGGA T^CCGGA
AflII 699 CTTAAG C^TTAAG
AhdI 2566 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2322 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 128 GGTACC G^GTACC
AspEI 2566 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1636 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3128 TGCGCA TGC^GCA
BalI 1335 TGGCCA TGG^CCA
BamHI 977 GGATCC G^GATCC
BbvCI 3321 GCTGAGG CCTCAGC(-5/-2)
BcgI 1491 CGATAATAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 617 GTATCC GTATCC(6/5)
BfrI 699 CTTAAG C^TTAAG
BlpI 770 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 770 GCTTAGC GC^T[ACGT]AGC
BsaWI 3116 TCCGGA [AT]^CCGG[AT]
BseAI 3116 TCCGGA T^CCGGA
BspEI 3116 TCCGGA T^CCGGA
BstAPI 1578 GCAAGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1943 TTCGAA TT^CGAA
CelII 770 GCTTAGC GC^T[ACGT]AGC
CfoI 3129 GCGC GCG^C
DrdI 1739 GACCCAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2285 CCATGG C^C[GA][CT]GG
Eam1105I 2566 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 3128 TGCGCA TGC^GCA
HhaI 3129 GCGC GCG^C
HinP1I 3129 GCGC G^CGC
HindIII 398 AAGCTT A^AGCTT
KpnI 128 GGTACC GGTAC^C
MluNI 1335 TGGCCA TGG^CCA
MroI 3116 TCCGGA T^CCGGA
MscI 1335 TGGCCA TGG^CCA
NcoI 2285 CCATGG C^CATGG
NspV 1943 TTCGAA TT^CGAA
PflFI 1636 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1962 CCATCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3416 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2204 GCTCTTC GCTCTTC(1/4)
ScaI 108 AGTACT AGT^ACT
SexAI 2860 ACCAGGT A^CC[AT]GGT
SfuI 1943 TTCGAA TT^CGAA
SpeI 2713 ACTAGT A^CTAGT
SphI 409 GCATGC GCATG^C
Tth111I 1636 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1962 CCATCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1818 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 20 enzymes) #
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AflIII 28 ACATGT A^C[GA][CT]GT
840 ACATGT
Asp700I 802 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1051 GAAACCCTTC
BsaBI 2095 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2844 GATGTTCATC
BseRI 1561 GAGGAG GAGGAG(10/8)
2290 GAGGAG
BspLU11I 28 ACATGT A^CATGT
840 ACATGT
BstEII 934 GGTTACC G^GT[ACGT]ACC
2025 GGTCACC
BstPI 934 GGTTACC G^GT[ACGT]ACC
2025 GGTCACC
DraI 676 TTTAAA TTT^AAA
2883 TTTAAA
EaeI 1335 TGGCCA [CT]^GGCC[GA]
1664 TGGCCG
EarI 2205 CTCTTC CTCTTC(1/4)
353 GAAGAG
EcoO65I 934 GGTTACC G^GT[ACGT]ACC
2025 GGTCACC
EcoRI 3094 GAATTC G^AATTC
3187 GAATTC
EcoRV 145 GATATC GAT^ATC
1291 GATATC
HgaI 1615 GACGC GACGC(5/10)
3471 GCGTC
Ksp632I 2205 CTCTTC CTCTTC(1/4)
353 GAAGAG
MamI 2095 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2844 GATGTTCATC
PaeR7I 1415 CTCGAG C^TCGAG
1993 CTCGAG
PpuMI 729 AGGACCC [GA]G^G[AT]CC[CT]
1737 AGGACCC
XhoI 1415 CTCGAG C^TCGAG
1993 CTCGAG
XmnI 802 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1051 GAAACCCTTC
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# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmFI
BsmI BsoBI Bsp1286I BspHI BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MspI MunI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII