Restriction map for
pF1KB9488
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BalI BamHI
BbeI BcgI BlnI BlpI Bpu1102I
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspHI BspLU11I BspMI BssHII
BssSI Bst1107I BstBI BstZ17I Bsu36I
CelII ClaI CpoI DsaI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI FseI FspI HaeII KasI
KpnI KspI MluI MluNI MroI
MscI NarI NcoI NheI NotI
NruI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SpeI SphI Sse8387I
SwaI Tth111I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 1346 CGCG CG^CG
AcyI 112 GACGCC G[GA]^CG[CT]C
AflII 2751 CTTAAG C^TTAAG
AhdI 1585 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2768 GGGCCC GGGCC^C
AspEI 1585 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2830 CTCGAG C^[CT]CG[GA]G
BanI 276 GGTGCC G^G[CT][GA]CC
BbvCI 2966 CCTCAGC CCTCAGC(-5/-2)
BclI 2237 TGATCA T^GATCA
BfrI 2751 CTTAAG C^TTAAG
BglI 2146 GCCTTACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1822 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 112 GACGCC G[GA]^CG[CT]C
BsaI 3178 GGTCTC GGTCTC(1/5)
BsmBI 2080 GAGACG CGTCTC(1/5)
BsoBI 2830 CTCGAG C^[CT]CG[GA]G
BsrBI 1075 GAGCGG CCGCTC(-3/-3)
BsrFI 41 GCCGGC [GA]^CCGG[CT]
BstEII 172 GGTGACC G^GT[ACGT]ACC
BstPI 172 GGTGACC G^GT[ACGT]ACC
BstUI 1346 CGCG CG^CG
Cfr10I 41 GCCGGC [GA]^CCGG[CT]
DraI 2100 TTTAAA TTT^AAA
DrdI 2322 GACTGTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1585 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 172 GGTGACC G^GT[ACGT]ACC
EcoRI 1244 GAATTC G^AATTC
EcoRV 1660 GATATC GAT^ATC
EcoT14I 1557 CCTTGG C^C[AT][AT]GG
FauI 1344 CCCGC CCCGC(4/6)
FbaI 2237 TGATCA T^GATCA
HgaI 112 GACGC GACGC(5/10)
Hin1I 112 GACGCC G[GA]^CG[CT]C
HpaI 1011 GTTAAC GTT^AAC
MamI 1822 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1346 CGCG CG^CG
NaeI 41 GCCGGC GCC^GGC
NdeI 212 CATATG CA^TATG
NgoMIV 41 GCCGGC G^CCGGC
PaeR7I 2830 CTCGAG C^TCGAG
PpuMI 2962 AGGACCT [GA]G^G[AT]CC[CT]
PstI 473 CTGCAG CTGCA^G
SacI 2791 GAGCTC GAGCT^C
SalI 3365 GTCGAC G^TCGAC
SnaBI 3197 TACGTA TAC^GTA
StuI 971 AGGCCT AGG^CCT
StyI 1557 CCTTGG C^C[AT][AT]GG
XcmI 2810 CCATGACCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2830 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 194 GTAGAC GT^[AC][GT]AC
3365 GTCGAC
AlwNI 1684 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3262 CAGCAGCTG
BbrPI 2416 CACGTG CAC^GTG
3016 CACGTG
BbsI 2409 GAAGAC GAAGAC(2/6)
2891 GTCTTC
BcnI 2457 CCCGG CC^[GC]GG
2936 CCGGG
BglII 830 AGATCT A^GATCT
3054 AGATCT
BpmI 2794 CTCCAG CTGGAG(16/14)
2864 CTGGAG
BpuAI 2409 GAAGAC GAAGAC(2/6)
2891 GTCTTC
BsmI 2881 GAATGC GAATGC(1/-1)
3048 GAATGC
Bsp1407I 759 TGTACA T^GTACA
2265 TGTACA
BsrGI 759 TGTACA T^GTACA
2265 TGTACA
BstAPI 1060 GCATACTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3219 GCAGGAAATGC
BstXI 353 CCACCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
969 CCAGGCCTTTGG
CfoI 350 GCGC GCG^C
727 GCGC
DraII 2768 GGGCCCT [GA]G^G[ACGT]CC[CT]
2962 AGGACCT
DraIII 9 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
538 CACCTTGTG
EcoO109I 2768 GGGCCCT [GA]G^G[ACGT]CC[CT]
2962 AGGACCT
EcoT22I 488 ATGCAT ATGCA^T
947 ATGCAT
HhaI 350 GCGC GCG^C
727 GCGC
HinP1I 350 GCGC G^CGC
727 GCGC
HincII 1011 GTTAAC GT[CT]^[GA]AC
3365 GTCGAC
HindII 1011 GTTAAC GT[CT]^[GA]AC
3365 GTCGAC
HindIII 1112 AAGCTT A^AGCTT
1412 AAGCTT
MfeI 872 CAATTG C^AATTG
2642 CAATTG
MunI 872 CAATTG C^AATTG
2642 CAATTG
NciI 2457 CCCGG CC^[GC]GG
2936 CCGGG
NsiI 488 ATGCAT ATGCA^T
947 ATGCAT
NspI 490 GCATGT [GA]CATG^[CT]
582 ACATGC
PmaCI 2416 CACGTG CAC^GTG
3016 CACGTG
PmlI 2416 CACGTG CAC^GTG
3016 CACGTG
SspBI 759 TGTACA T^GTACA
2265 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbvI
BciVI BfaI BmyI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PflMI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII