Restriction map for
pF1KB9487
##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII AgeI AhdI Aor51HI
AscI AspEI AspI AviII AvrII
BbeI BglI BglII BlnI BseAI
BsiEI BsiWI BspDI BspEI BspLU11I
BssHII Bst1107I BstZ17I ClaI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoT22I FseI FspI HindIII HpaI
KasI KspI MfeI MluI MroI
MunI NarI NdeI NotI NruI
NsiI PacI PflFI PinAI PmeI
PvuI SacII SapI SbfI ScaI
SfiI SfoI SgrAI SnaBI Sse8387I
SspI Tth111I
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
Acc65I 1569 GGTACC G^GTACC
AccII 1296 CGCG CG^CG
AclI 629 AACGTT AA^CGTT
AcyI 956 GACGCC G[GA]^CG[CT]C
AflII 1675 CTTAAG C^TTAAG
AflIII 2963 ACACGT A^C[GA][CT]GT
Alw44I 102 GTGCAC G^TGCAC
AocI 3465 CCTCAGG CC^T[ACGT]AGG
ApaI 2950 GGGCCC GGGCC^C
ApaLI 102 GTGCAC G^TGCAC
AseI 2887 ATTAAT AT^TAAT
AsnI 2887 ATTAAT AT^TAAT
Asp718I 1569 GGTACC G^GTACC
BamHI 1364 GGATCC G^GATCC
BbrPI 2964 CACGTG CAC^GTG
BclI 2537 TGATCA T^GATCA
BfrI 1675 CTTAAG C^TTAAG
BlpI 3092 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3092 GCTCAGC GC^T[ACGT]AGC
BsaAI 2964 CACGTG [CT]AC^GT[GA]
BsaHI 956 GACGCC G[GA]^CG[CT]C
BsaI 2481 GAGACC GGTCTC(1/5)
BsmBI 172 GAGACG CGTCTC(1/5)
BsmI 133 GAATGC GAATGC(1/-1)
BspHI 3485 TCATGA T^CATGA
BsrBI 2452 GAGCGG CCGCTC(-3/-3)
BssSI 2050 CACGAG CACGAG(-5/-1)
BstAPI 50 GCAAGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3131 TTCGAA TT^CGAA
BstUI 1296 CGCG CG^CG
BstXI 797 CCATTCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3465 CCTCAGG CC^T[ACGT]AGG
CelII 3092 GCTCAGC GC^T[ACGT]AGC
CpoI 3293 CGGTCCG CG^G[AT]CCG
DraI 1134 TTTAAA TTT^AAA
DraIII 1603 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1968 GACGCTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3465 CCTCAGG CC^T[ACGT]AGG
FbaI 2537 TGATCA T^GATCA
Hin1I 956 GACGCC G[GA]^CG[CT]C
KpnI 1569 GGTACC GGTAC^C
MvnI 1296 CGCG CG^CG
NheI 420 GCTAGC G^CTAGC
NspV 3131 TTCGAA TT^CGAA
PaeR7I 2627 CTCGAG C^TCGAG
PmaCI 2964 CACGTG CAC^GTG
PmlI 2964 CACGTG CAC^GTG
PpuMI 2054 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 2483 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2887 ATTAAT AT^TAAT
Psp1406I 629 AACGTT AA^CGTT
RcaI 3485 TCATGA T^CATGA
RsrII 3293 CGGTCCG CG^G[AT]CCG
SacI 808 GAGCTC GAGCT^C
SalI 792 GTCGAC G^TCGAC
SfuI 3131 TTCGAA TT^CGAA
SmaI 2666 CCCGGG CCC^GGG
SpeI 2440 ACTAGT A^CTAGT
SphI 3314 GCATGC GCATG^C
SwaI 1133 ATTTAAAT ATTT^AAAT
XbaI 2547 TCTAGA T^CTAGA
XhoI 2627 CTCGAG C^TCGAG
XmaCI 2666 CCCGGG C^CCGGG
XmaI 2666 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BbvCI 2340 GCTGAGG CCTCAGC(-5/-2)
3456 GCTGAGG
BcgI 1298 CGATCATCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
89 GCAATGTTATCG
BsaWI 1461 ACCGGA [AT]^CCGG[AT]
1842 TCCGGT
BsgI 921 CTGCAC GTGCAG(16/14)
1804 GTGCAG
BspMI 356 ACCTGC ACCTGC(4/8)
1722 ACCTGC
BsrDI 89 GCAATG GCAATG(2/0)
2279 GCAATG
BstEII 1791 GGTGACC G^GT[ACGT]ACC
3117 GGTCACC
BstPI 1791 GGTGACC G^GT[ACGT]ACC
3117 GGTCACC
EcoNI 519 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1310 CCTCGTACAGG
EcoO65I 1791 GGTGACC G^GT[ACGT]ACC
3117 GGTCACC
EcoRV 344 GATATC GAT^ATC
850 GATATC
NaeI 424 GCCGGC GCC^GGC
2388 GCCGGC
NgoMIV 424 GCCGGC G^CCGGC
2388 GCCGGC
PflMI 153 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1920 CCACAGCCTGG
PvuII 1636 CAGCTG CAG^CTG
2206 CAGCTG
SexAI 407 ACCAGGT A^CC[AT]GGT
1787 ACCTGGT
StuI 1214 AGGCCT AGG^CCT
2568 AGGCCT
Van91I 153 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1920 CCACAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BciVI BcnI BfaI
BmyI BpmI BpuAI BsaBI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI