Restriction map for
pF1KB9479
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BcgI BciVI
BclI BfrI BglII BlnI BsaAI
BsaBI BsaHI BseAI BsiEI BsiWI
BspDI BspEI BsrDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CfoI ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FbaI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI MamI MfeI MluI MroI
MunI NarI NdeI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacI SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
SspI SwaI TaqI TthHB8I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 1261 CGCG CG^CG
AcsI 1401 GAATTC [GA]^AATT[CT]
AflIII 587 ACATGT A^C[GA][CT]GT
ApoI 1401 GAATTC [GA]^AATT[CT]
Asp700I 880 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1462 GAGCAC G[AT]GC[AT]^C
AvaI 706 CCCGAG C^[CT]CG[GA]G
BalI 824 TGGCCA TGG^CCA
BbsI 806 GTCTTC GAAGAC(2/6)
BpuAI 806 GTCTTC GAAGAC(2/6)
BsaWI 1451 ACCGGA [AT]^CCGG[AT]
BsgI 557 GTGCAG GTGCAG(16/14)
BsiHKAI 1462 GAGCAC G[AT]GC[AT]^C
BsmBI 480 GAGACG CGTCTC(1/5)
BsoBI 706 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1221 TGTACA T^GTACA
BspHI 1950 TCATGA T^CATGA
BspLU11I 587 ACATGT A^CATGT
BsrBI 685 GAGCGG CCGCTC(-3/-3)
BsrFI 499 GCCGGC [GA]^CCGG[CT]
BsrGI 1221 TGTACA T^GTACA
BstAPI 993 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1261 CGCG CG^CG
BstYI 1230 GGATCT [GA]^GATC[CT]
Cfr10I 499 GCCGGC [GA]^CCGG[CT]
DraIII 1079 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 280 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1401 GAATTC G^AATTC
FseI 498 GGCCGGCC GGCCGG^CC
HgaI 180 GACGC GACGC(5/10)
KspI 1260 CCGCGG CCGC^GG
MaeII 458 ACGT A^CGT
MflI 1230 GGATCT [GA]^GATC[CT]
MluNI 824 TGGCCA TGG^CCA
MscI 824 TGGCCA TGG^CCA
MseI 1180 TTAA T^TAA
MvnI 1261 CGCG CG^CG
NaeI 499 GCCGGC GCC^GGC
NgoMIV 499 GCCGGC G^CCGGC
NheI 1818 GCTAGC G^CTAGC
NspI 587 ACATGT [GA]CATG^[CT]
PshAI 231 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1950 TCATGA T^CATGA
SacII 1260 CCGCGG CCGC^GG
SspBI 1221 TGTACA T^GTACA
Tru9I 1180 TTAA T^TAA
XhoII 1230 GGATCT [GA]^GATC[CT]
XmnI 880 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 1222 GTAC GT^AC
1743 GTAC
AlwI 413 GGATC GGATC(4/5)
1230 GGATC
AspI 778 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1030 GACTGTGTC
BbvCI 508 CCTCAGC CCTCAGC(-5/-2)
1165 GCTGAGG
BlpI 1288 GCTGAGC GC^T[ACGT]AGC
1459 GCTGAGC
Bpu1102I 1288 GCTGAGC GC^T[ACGT]AGC
1459 GCTGAGC
BsmI 927 GAATGC GAATGC(1/-1)
215 GCATTC
CelII 1288 GCTGAGC GC^T[ACGT]AGC
1459 GCTGAGC
DpnII 414 GATC ^GATC
1231 GATC
DrdI 292 GACACAGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1345 GACCCTGGGGTC
EaeI 824 TGGCCA [CT]^GGCC[GA]
1491 TGGCCG
MboI 414 GATC ^GATC
1231 GATC
MslI 324 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
453 CACCGACGTG
NdeII 414 GATC ^GATC
1231 GATC
PflFI 778 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1030 GACTGTGTC
PflMI 1014 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1299 CCACAGATTGG
RsaI 1222 GTAC GT^AC
1743 GTAC
SapI 880 GAAGAGC GCTCTTC(1/4)
183 GCTCTTC
Sau3AI 414 GATC ^GATC
1231 GATC
SbfI 111 CCTGCAGG CCTGCA^GG
312 CCTGCAGG
Sse8387I 111 CCTGCAGG CCTGCA^GG
312 CCTGCAGG
StuI 336 AGGCCT AGG^CCT
1373 AGGCCT
TfiI 97 GAATC G^A[AT]TC
1486 GAATC
Tsp509I 152 AATT ^AATT
1402 AATT
Tth111I 778 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1030 GACTGTGTC
Van91I 1014 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1299 CCACAGATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AluI AlwNI ApaI AvaII
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI Cac8I Cfr13I DdeI
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboII MnlI
MspA1I MspI MvaI MwoI NciI
NcoI NlaIII NlaIV PleI PpuMI
PstI PvuII Sau96I ScrFI SfaNI
SfcI StyI TseI Tsp45I TspRI
XcmI