Restriction map for
pF1KB9478
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
AhdI AocI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BclI BlnI BsaAI BsaBI
BsaHI BsiEI BsiWI BsmBI BspDI
BspHI BsrFI BssHII Bst1107I BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRV
FbaI FseI FspI Hin1I HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SfiI SfoI SgrAI SnaBI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 3052 GTCTAC GT^[AC][GT]AC
AccII 2064 CGCG CG^CG
AflII 2644 CTTAAG C^TTAAG
AflIII 176 ACATGT A^C[GA][CT]GT
BciVI 2315 GGATAC GTATCC(6/5)
BfrI 2644 CTTAAG C^TTAAG
BglII 3069 AGATCT A^GATCT
Bsp1407I 20 TGTACA T^GTACA
BspLU11I 176 ACATGT A^CATGT
BsrBI 2251 CCGCTC CCGCTC(-3/-3)
BsrGI 20 TGTACA T^GTACA
BstAPI 3152 GCATGCGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 44 TTCGAA TT^CGAA
BstEII 529 GGTGACC G^GT[ACGT]ACC
BstPI 529 GGTGACC G^GT[ACGT]ACC
BstUI 2064 CGCG CG^CG
EcoO65I 529 GGTGACC G^GT[ACGT]ACC
EcoRI 3514 GAATTC G^AATTC
HaeII 3117 AGCGCC [GA]GCGC^[CT]
HindIII 2277 AAGCTT A^AGCTT
MaeII 1255 ACGT A^CGT
MvnI 2064 CGCG CG^CG
NcoI 887 CCATGG C^CATGG
NspV 44 TTCGAA TT^CGAA
PflMI 2175 CCAAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1557 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1861 CTGCAG CTGCA^G
SapI 536 GAAGAGC GCTCTTC(1/4)
SbfI 1860 CCTGCAGG CCTGCA^GG
ScaI 214 AGTACT AGT^ACT
SexAI 3575 ACCTGGT A^CC[AT]GGT
SfuI 44 TTCGAA TT^CGAA
SphI 3152 GCATGC GCATG^C
Sse8387I 1860 CCTGCAGG CCTGCA^GG
SspBI 20 TGTACA T^GTACA
SspI 3135 AATATT AAT^ATT
StuI 1829 AGGCCT AGG^CCT
Van91I 2175 CCAAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 1506 TCCGGA T^CCGGA
2797 TCCGGA
Alw44I 3543 GTGCAC G^TGCAC
3580 GTGCAC
AlwI 1681 GATCC GGATC(4/5)
483 GGATC
ApaLI 3543 GTGCAC G^TGCAC
3580 GTGCAC
BalI 2429 TGGCCA TGG^CCA
2676 TGGCCA
BanI 1619 GGCACC G^G[CT][GA]CC
3267 GGCACC
BbsI 1270 GAAGAC GAAGAC(2/6)
1935 GAAGAC
BlpI 1138 GCTGAGC GC^T[ACGT]AGC
3528 GCTCAGC
Bpu1102I 1138 GCTGAGC GC^T[ACGT]AGC
3528 GCTCAGC
BpuAI 1270 GAAGAC GAAGAC(2/6)
1935 GAAGAC
BseAI 1506 TCCGGA T^CCGGA
2797 TCCGGA
BsmI 302 GCATTC GAATGC(1/-1)
1665 GCATTC
BspEI 1506 TCCGGA T^CCGGA
2797 TCCGGA
BssSI 1653 CACGAG CACGAG(-5/-1)
3498 CACGAG
BstYI 1680 AGATCC [GA]^GATC[CT]
3069 AGATCT
CelII 1138 GCTGAGC GC^T[ACGT]AGC
3528 GCTCAGC
EcoT22I 3150 ATGCAT ATGCA^T
3279 ATGCAT
MflI 1680 AGATCC [GA]^GATC[CT]
3069 AGATCT
MluNI 2429 TGGCCA TGG^CCA
2676 TGGCCA
MroI 1506 TCCGGA T^CCGGA
2797 TCCGGA
MscI 2429 TGGCCA TGG^CCA
2676 TGGCCA
NsiI 3150 ATGCAT ATGCA^T
3279 ATGCAT
SacI 2019 GAGCTC GAGCT^C
2406 GAGCTC
SfcI 1861 CTGCAG C^T[GA][CT]AG
3065 CTGTAG
SpeI 325 ACTAGT A^CTAGT
2161 ACTAGT
XhoII 1680 AGATCC [GA]^GATC[CT]
3069 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApaI ApoI Asp700I AspHI AvaI
AvaII BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XmaCI XmaI
XmnI