Restriction map for
pF1KB9476
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Aor51HI AscI
AseI AsnI AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmAI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII Bst1107I BstEII BstPI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KasI MaeII MaeIII MamI
MfeI MluI MroI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
ScaI SexAI SfoI SgrAI SnaBI
SpeI SphI SspBI SspI SwaI
TfiI Tsp45I Tth111I Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 398 GGTACC G^GTACC
AcsI 43 GAATTC [GA]^AATT[CT]
AcyI 184 GACGCC G[GA]^CG[CT]C
AlwNI 517 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 550 CCTGAGG CC^T[ACGT]AGG
ApaI 981 GGGCCC GGGCC^C
ApoI 43 GAATTC [GA]^AATT[CT]
Asp700I 39 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 398 GGTACC G^GTACC
AvaII 615 GGACC G^G[AT]CC
BalI 342 TGGCCA TGG^CCA
BbsI 486 GAAGAC GAAGAC(2/6)
BfaI 619 CTAG C^TAG
BpuAI 486 GAAGAC GAAGAC(2/6)
BsaHI 184 GACGCC G[GA]^CG[CT]C
BseRI 835 CTCCTC GAGGAG(10/8)
BsgI 789 GTGCAG GTGCAG(16/14)
BsmFI 367 GGGAC GGGAC(10/14)
BsrBI 622 GAGCGG CCGCTC(-3/-3)
BsrI 133 CCAGT ACTGG(1/-1)
BssSI 109 CTCGTG CACGAG(-5/-1)
BstAPI 105 GCAGCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 241 TTCGAA TT^CGAA
BstYI 467 AGATCC [GA]^GATC[CT]
Bsu36I 550 CCTGAGG CC^T[ACGT]AGG
Eco81I 550 CCTGAGG CC^T[ACGT]AGG
EcoRI 43 GAATTC G^AATTC
HaeII 1007 AGCGCC [GA]GCGC^[CT]
HgaI 184 GACGC GACGC(5/10)
Hin1I 184 GACGCC G[GA]^CG[CT]C
HincII 592 GTCAAC GT[CT]^[GA]AC
HindII 592 GTCAAC GT[CT]^[GA]AC
KpnI 398 GGTACC GGTAC^C
KspI 1017 CCGCGG CCGC^GG
MaeI 619 CTAG C^TAG
MflI 467 AGATCC [GA]^GATC[CT]
MluNI 342 TGGCCA TGG^CCA
MscI 342 TGGCCA TGG^CCA
MseI 476 TTAA T^TAA
NspV 241 TTCGAA TT^CGAA
SacII 1017 CCGCGG CCGC^GG
SbfI 522 CCTGCAGG CCTGCA^GG
SfiI 306 GGCCACGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 241 TTCGAA TT^CGAA
Sse8387I 522 CCTGCAGG CCTGCA^GG
StuI 962 AGGCCT AGG^CCT
Tru9I 476 TTAA T^TAA
XcmI 133 CCAGTAGGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 467 AGATCC [GA]^GATC[CT]
XmnI 39 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Alw44I 643 GTGCAC G^TGCAC
934 GTGCAC
AlwI 468 GATCC GGATC(4/5)
147 GGATC
ApaLI 643 GTGCAC G^TGCAC
934 GTGCAC
BsmI 228 GAATGC GAATGC(1/-1)
806 GAATGC
BspMI 203 ACCTGC ACCTGC(4/8)
429 ACCTGC
BstNI 260 CCTGG CC^[AT]GG
985 CCAGG
BstXI 632 CCATTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
656 CCACGGATGTGG
CfoI 4 GCGC GCG^C
1008 GCGC
DraII 527 AGGGCCT [GA]G^G[ACGT]CC[CT]
980 AGGGCCC
EaeI 305 CGGCCA [CT]^GGCC[GA]
342 TGGCCA
EcoO109I 527 AGGGCCT [GA]G^G[ACGT]CC[CT]
980 AGGGCCC
EcoRII 260 CCTGG ^CC[AT]GG
985 CCAGG
EcoT14I 53 CCAAGG C^C[AT][AT]GG
977 CCAAGG
HhaI 4 GCGC GCG^C
1008 GCGC
HinP1I 4 GCGC G^CGC
1008 GCGC
HphI 483 GGTGA GGTGA(8/7)
1027 GGTGA
MvaI 260 CCTGG CC^[AT]GG
985 CCAGG
NlaIII 733 CATG CATG^
774 CATG
PstI 523 CTGCAG CTGCA^G
544 CTGCAG
PvuII 670 CAGCTG CAG^CTG
819 CAGCTG
SacI 358 GAGCTC GAGCT^C
457 GAGCTC
SmaI 680 CCCGGG CCC^GGG
1023 CCCGGG
SmlI 577 CTCAAG C^T[CT][GA]AG
748 CTTGAG
StyI 53 CCAAGG C^C[AT][AT]GG
977 CCAAGG
Tsp509I 44 AATT ^AATT
882 AATT
XmaCI 680 CCCGGG C^CCGGG
1023 CCCGGG
XmaI 680 CCCGGG C^CCGGG
1023 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaI BanI BanII BbvI BcnI
BglI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsoBI Bsp1286I BssKI
BstF5I BstUI Cac8I Cfr13I DdeI
DpnII DsaI EarI FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI Ksp632I MboI MboII MnlI
MslI MspA1I MspI MvnI MwoI
NciI NdeII NlaIV PleI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TseI TspRI TthHB8I