Restriction map for
pF1KB9473
##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BcgI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsgI
BsiWI BspDI BspLU11I BsrFI BssSI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI KpnI KspI MfeI MluI
MunI NaeI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspI StuI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 151 GTCTAC GT^[AC][GT]AC
Asp700I 498 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2258 TGGCCA TGG^CCA
BanII 1571 GAGCCC G[GA]GC[CT]^C
BbeI 133 GGCGCC GGCGC^C
BbrPI 1510 CACGTG CAC^GTG
BciVI 2027 GTATCC GTATCC(6/5)
BpmI 1128 CTCCAG CTGGAG(16/14)
BsaAI 1510 CACGTG [CT]AC^GT[GA]
BsaBI 139 GATTTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2330 CGGCCG CG[GA][CT]^CG
BsmBI 409 GAGACG CGTCTC(1/5)
BsmI 2360 GCATTC GAATGC(1/-1)
Bsp1407I 1733 TGTACA T^GTACA
BspHI 2204 TCATGA T^CATGA
BspMI 318 GCAGGT ACCTGC(4/8)
BsrBI 2242 CCGCTC CCGCTC(-3/-3)
BsrGI 1733 TGTACA T^GTACA
BssHII 32 GCGCGC G^CGCGC
BstEII 1839 GGTCACC G^GT[ACGT]ACC
BstPI 1839 GGTCACC G^GT[ACGT]ACC
BstXI 581 CCAAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 685 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2330 CGGCCG C^GGCCG
EclXI 2330 CGGCCG C^GGCCG
Eco52I 2330 CGGCCG C^GGCCG
EcoO65I 1839 GGTCACC G^GT[ACGT]ACC
HindIII 1026 AAGCTT A^AGCTT
KasI 133 GGCGCC G^GCGCC
MamI 139 GATTTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2258 TGGCCA TGG^CCA
MscI 2258 TGGCCA TGG^CCA
NarI 133 GGCGCC GG^CGCC
NheI 1920 GCTAGC G^CTAGC
PmaCI 1510 CACGTG CAC^GTG
PmlI 1510 CACGTG CAC^GTG
PshAI 885 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2204 TCATGA T^CATGA
SexAI 218 ACCAGGT A^CC[AT]GGT
SfoI 133 GGCGCC GGC^GCC
SphI 1754 GCATGC GCATG^C
SspBI 1733 TGTACA T^GTACA
XcmI 452 CCATGGCACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 498 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 512 TCCGGA T^CCGGA
1253 TCCGGA
AlwNI 424 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
2310 CAGCAGCTG
AvaI 64 CCCGGG C^[CT]CG[GA]G
2339 CCCGGG
BbsI 940 GTCTTC GAAGAC(2/6)
2491 GTCTTC
BbvCI 171 CCTCAGC CCTCAGC(-5/-2)
1926 CCTCAGC
BfaI 484 CTAG C^TAG
1921 CTAG
BpuAI 940 GTCTTC GAAGAC(2/6)
2491 GTCTTC
BsaI 2477 GAGACC GGTCTC(1/5)
1627 GGTCTC
BseAI 512 TCCGGA T^CCGGA
1253 TCCGGA
BsoBI 64 CCCGGG C^[CT]CG[GA]G
2339 CCCGGG
BspEI 512 TCCGGA T^CCGGA
1253 TCCGGA
BstYI 817 GGATCT [GA]^GATC[CT]
2483 AGATCC
DraII 414 GGGACCC [GA]G^G[ACGT]CC[CT]
2228 AGGTCCT
EaeI 2258 TGGCCA [CT]^GGCC[GA]
2330 CGGCCG
EcoO109I 414 GGGACCC [GA]G^G[ACGT]CC[CT]
2228 AGGTCCT
HaeII 133 GGCGCC [GA]GCGC^[CT]
1798 AGCGCC
HincII 2092 GTTAAC GT[CT]^[GA]AC
2442 GTTAAC
HindII 2092 GTTAAC GT[CT]^[GA]AC
2442 GTTAAC
HpaI 2092 GTTAAC GTT^AAC
2442 GTTAAC
MaeI 484 CTAG C^TAG
1921 CTAG
MflI 817 GGATCT [GA]^GATC[CT]
2483 AGATCC
MroI 512 TCCGGA T^CCGGA
1253 TCCGGA
NcoI 452 CCATGG C^CATGG
2006 CCATGG
NspI 1754 GCATGC [GA]CATG^[CT]
2150 ACATGC
PflMI 684 CCACAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1905 CCACGTTCTGG
PleI 592 GACTC GAGTC(4/5)
1120 GAGTC
PpuMI 414 GGGACCC [GA]G^G[AT]CC[CT]
2228 AGGTCCT
PvuII 1381 CAGCTG CAG^CTG
2313 CAGCTG
SapI 330 GCTCTTC GCTCTTC(1/4)
2244 GCTCTTC
SmaI 64 CCCGGG CCC^GGG
2339 CCCGGG
Van91I 684 CCACAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1905 CCACGTTCTGG
XhoII 817 GGATCT [GA]^GATC[CT]
2483 AGATCC
XmaCI 64 CCCGGG C^CCGGG
2339 CCCGGG
XmaI 64 CCCGGG C^CCGGG
2339 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaII
BanI BbvI BcnI BmyI BsaHI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I