Restriction map for
pF1KB9469
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccI AclI AflIII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp718I AviII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BglI BsaAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspLU11I
BspMI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Cfr10I ClaI CpoI
DraI DraIII EagI EclXI Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MluNI MscI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 356 TCCGGA T^CCGGA
AcyI 900 GACGCC G[GA]^CG[CT]C
AflII 745 CTTAAG C^TTAAG
AhdI 1514 GACACTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 152 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1514 GACACTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 975 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1443 CCTAGG C^CTAGG
BfrI 745 CTTAAG C^TTAAG
BglII 1685 AGATCT A^GATCT
BlnI 1443 CCTAGG C^CTAGG
BlpI 925 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 925 GCTAAGC GC^T[ACGT]AGC
BsaBI 1629 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 900 GACGCC G[GA]^CG[CT]C
BsaWI 356 TCCGGA [AT]^CCGG[AT]
BseAI 356 TCCGGA T^CCGGA
BseRI 1923 GAGGAG GAGGAG(10/8)
BsgI 1715 CTGCAC GTGCAG(16/14)
BsmI 1263 GAATGC GAATGC(1/-1)
BspEI 356 TCCGGA T^CCGGA
BspHI 1379 TCATGA T^CATGA
BsrBI 385 GAGCGG CCGCTC(-3/-3)
BsrDI 2297 GCAATG GCAATG(2/0)
CelII 925 GCTAAGC GC^T[ACGT]AGC
DrdI 186 GACGCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1085 CCATGG C^C[GA][CT]GG
EaeI 1619 TGGCCG [CT]^GGCC[GA]
Eam1105I 1514 GACACTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 730 GAAGAG CTCTTC(1/4)
Hin1I 900 GACGCC G[GA]^CG[CT]C
Ksp632I 730 GAAGAG CTCTTC(1/4)
MamI 1629 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 356 TCCGGA T^CCGGA
MspA1I 13 CCGCTG C[AC]G^C[GT]G
NcoI 1085 CCATGG C^CATGG
NspI 775 ACATGC [GA]CATG^[CT]
PflFI 975 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2080 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1379 TCATGA T^CATGA
ScaI 956 AGTACT AGT^ACT
SexAI 2338 ACCAGGT A^CC[AT]GGT
SmaI 725 CCCGGG CCC^GGG
StuI 1963 AGGCCT AGG^CCT
Tth111I 975 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2080 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 896 CCATGACGCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 725 CCCGGG C^CCGGG
XmaI 725 CCCGGG C^CCGGG
XmnI 152 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 55 CGCG CG^CG
132 CGCG
AocI 1356 CCTCAGG CC^T[ACGT]AGG
1702 CCTGAGG
AvaI 725 CCCGGG C^[CT]CG[GA]G
1736 CTCGGG
BbsI 972 GAAGAC GAAGAC(2/6)
453 GTCTTC
BpuAI 972 GAAGAC GAAGAC(2/6)
453 GTCTTC
BsoBI 725 CCCGGG C^[CT]CG[GA]G
1736 CTCGGG
BstUI 55 CGCG CG^CG
132 CGCG
BstYI 1685 AGATCT [GA]^GATC[CT]
2235 AGATCC
Bsu36I 1356 CCTCAGG CC^T[ACGT]AGG
1702 CCTGAGG
Eco81I 1356 CCTCAGG CC^T[ACGT]AGG
1702 CCTGAGG
FauI 12 CCCGC CCCGC(4/6)
2302 GCGGG
HgaI 186 GACGC GACGC(5/10)
900 GACGC
MaeII 113 ACGT A^CGT
2006 ACGT
MflI 1685 AGATCT [GA]^GATC[CT]
2235 AGATCC
MvnI 55 CGCG CG^CG
132 CGCG
SacI 646 GAGCTC GAGCT^C
1601 GAGCTC
Tsp45I 1313 GTGAC ^GT[GC]AC
1741 GTGAC
XhoII 1685 AGATCT [GA]^GATC[CT]
2235 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI Aor51HI ApaI ApoI AspHI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
Eco47III EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I