Restriction map for
pF1KB9468
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# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AgeI AhdI ApaI AscI
Asp718I AspEI AspI AviII AvrII
BbrPI BbvCI BciVI BglI BlnI
BsaAI BsiEI BsiWI Bsp1407I BspDI
BspLU11I BsrBI BsrGI BstBI ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco52I EcoNI EcoRV FseI FspI
HpaI KpnI KspI MfeI MluI
MunI NdeI NotI NruI NspV
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PvuI RsrII
SacII SalI SbfI SfiI SfuI
SgrAI SnaBI SphI Sse8387I SspBI
SwaI Tth111I XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 3 GACGTC GACGT^C
AccIII 3945 TCCGGA T^CCGGA
AflIII 4164 ACACGT A^C[GA][CT]GT
Alw44I 3265 GTGCAC G^TGCAC
Aor51HI 4854 AGCGCT AGC^GCT
ApaLI 3265 GTGCAC G^TGCAC
BbeI 4181 GGCGCC GGCGC^C
BsaWI 3945 TCCGGA [AT]^CCGG[AT]
BseAI 3945 TCCGGA T^CCGGA
BspEI 3945 TCCGGA T^CCGGA
BspHI 1514 TCATGA T^CATGA
BspMI 5267 ACCTGC ACCTGC(4/8)
BsrFI 5863 GCCGGC [GA]^CCGG[CT]
BssSI 415 CACGAG CACGAG(-5/-1)
Bst1107I 5013 GTATAC GTA^TAC
BstEII 5160 GGTGACC G^GT[ACGT]ACC
BstPI 5160 GGTGACC G^GT[ACGT]ACC
BstXI 5240 CCAGCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 5013 GTATAC GTA^TAC
Cfr10I 5863 GCCGGC [GA]^CCGG[CT]
Eco47III 4854 AGCGCT AGC^GCT
EcoO65I 5160 GGTGACC G^GT[ACGT]ACC
EcoT22I 6141 ATGCAT ATGCA^T
HgaI 5395 GCGTC GACGC(5/10)
KasI 4181 GGCGCC G^GCGCC
MroI 3945 TCCGGA T^CCGGA
NaeI 5863 GCCGGC GCC^GGC
NarI 4181 GGCGCC GG^CGCC
NgoMIV 5863 GCCGGC G^CCGGC
NsiI 6141 ATGCAT ATGCA^T
NspI 1046 ACATGC [GA]CATG^[CT]
PacI 1747 TTAATTAA TTAAT^TAA
RcaI 1514 TCATGA T^CATGA
SexAI 5900 ACCAGGT A^CC[AT]GGT
SfoI 4181 GGCGCC GGC^GCC
SmaI 618 CCCGGG CCC^GGG
SpeI 5377 ACTAGT A^CTAGT
StuI 4632 AGGCCT AGG^CCT
XmaCI 618 CCCGGG C^CCGGG
XmaI 618 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 36 enzymes) #
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AccI 5013 GTATAC GT^[AC][GT]AC
5263 GTCTAC
AclI 2106 AACGTT AA^CGTT
4111 AACGTT
AcyI 3 GACGTC G[GA]^CG[CT]C
4181 GGCGCC
AflII 319 CTTAAG C^TTAAG
1726 CTTAAG
AocI 97 CCTAAGG CC^T[ACGT]AGG
6232 CCTAAGG
AvaI 618 CCCGGG C^[CT]CG[GA]G
5456 CCCGAG
BamHI 2285 GGATCC G^GATCC
3231 GGATCC
BcgI 4302 CGAAAAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
6148 GCACAGTTTTCG
BfrI 319 CTTAAG C^TTAAG
1726 CTTAAG
BlpI 626 GCTCAGC GC^T[ACGT]AGC
1452 GCTCAGC
Bpu1102I 626 GCTCAGC GC^T[ACGT]AGC
1452 GCTCAGC
BsaBI 746 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4389 GATGAATATC
BsaHI 3 GACGTC G[GA]^CG[CT]C
4181 GGCGCC
BsaI 4654 GAGACC GGTCTC(1/5)
5250 GGTCTC
BsmI 3293 GAATGC GAATGC(1/-1)
5342 GCATTC
BsoBI 618 CCCGGG C^[CT]CG[GA]G
5456 CCCGAG
BstAPI 1041 GCATTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3546 GCAAAAAATGC
Bsu36I 97 CCTAAGG CC^T[ACGT]AGG
6232 CCTAAGG
CelII 626 GCTCAGC GC^T[ACGT]AGC
1452 GCTCAGC
Eco81I 97 CCTAAGG CC^T[ACGT]AGG
6232 CCTAAGG
EcoRI 837 GAATTC G^AATTC
1484 GAATTC
Hin1I 3 GACGTC G[GA]^CG[CT]C
4181 GGCGCC
HincII 3073 GTCAAC GT[CT]^[GA]AC
5799 GTTGAC
HindII 3073 GTCAAC GT[CT]^[GA]AC
5799 GTTGAC
MamI 746 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4389 GATGAATATC
NheI 216 GCTAGC G^CTAGC
1189 GCTAGC
PflMI 1179 CCATTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3802 CCAGATGGTGG
Psp1406I 2106 AACGTT AA^CGTT
4111 AACGTT
SacI 2320 GAGCTC GAGCT^C
5575 GAGCTC
SapI 2317 GAAGAGC GCTCTTC(1/4)
5572 GAAGAGC
ScaI 3060 AGTACT AGT^ACT
4403 AGTACT
SspI 426 AATATT AAT^ATT
3939 AATATT
TaqI 116 TCGA T^CGA
6240 TCGA
TthHB8I 116 TCGA T^CGA
6240 TCGA
Van91I 1179 CCATTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3802 CCAGATGGTGG
XcmI 2290 CCAGTGGAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2826 CCAGGCCCTCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaII BalI BanI
BanII BbsI BbvI BclI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BsrDI BsrI BssHII BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XbaI XhoII XmnI