Restriction map for
pF1KB9467
##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccIII AgeI AhdI
AocI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BlnI BlpI Bpu1102I BsaAI
BseAI BsiEI BsiWI BspDI BspEI
BspHI BsrBI BssHII BssSI BstAPI
BstBI Bsu36I CelII ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoT22I
FauI FseI FspI KasI KpnI
KspI MluI MroI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 24 CGCG CG^CG
AclI 3261 AACGTT AA^CGTT
AcyI 2715 GGCGTC G[GA]^CG[CT]C
AflII 2736 CTTAAG C^TTAAG
AlwNI 797 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 51 AGCGCT AGC^GCT
Asp700I 3152 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1816 GGATCC G^GATCC
BclI 888 TGATCA T^GATCA
BfrI 2736 CTTAAG C^TTAAG
BglI 1634 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2824 AGATCT A^GATCT
BsaHI 2715 GGCGTC G[GA]^CG[CT]C
BsaWI 121 TCCGGT [AT]^CCGG[AT]
BsmBI 2717 CGTCTC CGTCTC(1/5)
BsrDI 411 GCAATG GCAATG(2/0)
BstEII 1450 GGTGACC G^GT[ACGT]ACC
BstPI 1450 GGTGACC G^GT[ACGT]ACC
BstUI 24 CGCG CG^CG
Eco47III 51 AGCGCT AGC^GCT
EcoO65I 1450 GGTGACC G^GT[ACGT]ACC
EcoRI 1743 GAATTC G^AATTC
EcoRV 3064 GATATC GAT^ATC
FbaI 888 TGATCA T^GATCA
HaeII 51 AGCGCT [GA]GCGC^[CT]
HgaI 2716 GCGTC GACGC(5/10)
Hin1I 2715 GGCGTC G[GA]^CG[CT]C
HindIII 1973 AAGCTT A^AGCTT
HpaI 2466 GTTAAC GTT^AAC
MfeI 1670 CAATTG C^AATTG
MunI 1670 CAATTG C^AATTG
MvnI 24 CGCG CG^CG
PflMI 3230 CCAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1577 GAGTC GAGTC(4/5)
Psp1406I 3261 AACGTT AA^CGTT
SacI 3532 GAGCTC GAGCT^C
ScaI 1847 AGTACT AGT^ACT
SspI 114 AATATT AAT^ATT
StuI 600 AGGCCT AGG^CCT
SwaI 1880 ATTTAAAT ATTT^AAAT
TaqI 1656 TCGA T^CGA
TthHB8I 1656 TCGA T^CGA
Van91I 3230 CCAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3152 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AflIII 271 ACATGT A^C[GA][CT]GT
388 ACATGT
Alw44I 1535 GTGCAC G^TGCAC
2881 GTGCAC
ApaLI 1535 GTGCAC G^TGCAC
2881 GTGCAC
BalI 1242 TGGCCA TGG^CCA
2081 TGGCCA
BanI 1915 GGTGCC G^G[CT][GA]CC
2168 GGTGCC
BanII 2236 GAGCCC G[GA]GC[CT]^C
3532 GAGCTC
BbsI 1230 GAAGAC GAAGAC(2/6)
2338 GTCTTC
BpuAI 1230 GAAGAC GAAGAC(2/6)
2338 GTCTTC
BsaBI 2117 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3064 GATATCAATC
BsaI 3523 GAGACC GGTCTC(1/5)
97 GGTCTC
BseRI 1367 CTCCTC GAGGAG(10/8)
1109 GAGGAG
BsmI 2013 GAATGC GAATGC(1/-1)
305 GCATTC
Bsp1407I 594 TGTACA T^GTACA
1544 TGTACA
BspLU11I 271 ACATGT A^CATGT
388 ACATGT
BspMI 489 ACCTGC ACCTGC(4/8)
3175 ACCTGC
BsrFI 1384 ACCGGC [GA]^CCGG[CT]
2568 GCCGGT
BsrGI 594 TGTACA T^GTACA
1544 TGTACA
Bst1107I 1756 GTATAC GTA^TAC
2985 GTATAC
BstYI 1816 GGATCC [GA]^GATC[CT]
2824 AGATCT
BstZ17I 1756 GTATAC GTA^TAC
2985 GTATAC
CfoI 25 GCGC GCG^C
52 GCGC
Cfr10I 1384 ACCGGC [GA]^CCGG[CT]
2568 GCCGGT
HhaI 25 GCGC GCG^C
52 GCGC
HinP1I 25 GCGC G^CGC
52 GCGC
MamI 2117 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3064 GATATCAATC
MflI 1816 GGATCC [GA]^GATC[CT]
2824 AGATCT
MluNI 1242 TGGCCA TGG^CCA
2081 TGGCCA
MscI 1242 TGGCCA TGG^CCA
2081 TGGCCA
PpuMI 740 GGGACCT [GA]G^G[AT]CC[CT]
1858 AGGTCCT
PvuII 2420 CAGCTG CAG^CTG
3287 CAGCTG
SmlI 2426 CTCAAG C^T[CT][GA]AG
2736 CTTAAG
SspBI 594 TGTACA T^GTACA
1544 TGTACA
XcmI 172 CCAAAAGGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2355 CCACAAAGACTGTGG
XhoII 1816 GGATCC [GA]^GATC[CT]
2824 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BbvI BfaI BmyI BpmI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI Cac8I Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PshAI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI