Restriction map for
pF1KB9465
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BciVI BclI BfrI
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DraIII Eam1105I Eco47III Eco81I
EcoRV EcoT22I FbaI FseI FspI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NarI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 620 ACATGT A^C[GA][CT]GT
AseI 142 ATTAAT AT^TAAT
AsnI 142 ATTAAT AT^TAAT
BamHI 2465 GGATCC G^GATCC
BcnI 774 CCGGG CC^[GC]GG
BsgI 414 GTGCAG GTGCAG(16/14)
BsiEI 1277 CGGCCG CG[GA][CT]^CG
BspHI 2721 TCATGA T^CATGA
BspLU11I 620 ACATGT A^CATGT
BsrDI 2106 CATTGC GCAATG(2/0)
BstAPI 1257 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI 1277 CGGCCG C^GGCCG
EclXI 1277 CGGCCG C^GGCCG
Eco52I 1277 CGGCCG C^GGCCG
EcoRI 1291 GAATTC G^AATTC
HaeII 73 GGCGCT [GA]GCGC^[CT]
HindIII 721 AAGCTT A^AGCTT
HpaI 549 GTTAAC GTT^AAC
NciI 774 CCGGG CC^[GC]GG
NcoI 1490 CCATGG C^CATGG
NheI 2734 GCTAGC G^CTAGC
NspI 620 ACATGT [GA]CATG^[CT]
PshBI 142 ATTAAT AT^TAAT
RcaI 2721 TCATGA T^CATGA
SapI 1777 GCTCTTC GCTCTTC(1/4)
ScaI 839 AGTACT AGT^ACT
SexAI 2342 ACCTGGT A^CC[AT]GGT
XbaI 1602 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 64 CGCG CG^CG
97 CGCG
AcyI 1909 GACGCC G[GA]^CG[CT]C
2101 GACGCC
AvaI 2423 CCCGAG C^[CT]CG[GA]G
2816 CTCGGG
BbvCI 902 CCTCAGC CCTCAGC(-5/-2)
3043 GCTGAGG
BcgI 2100 CGACGCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2808 GCAGGCCTCTCG
BglI 2200 GCCTACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2623 GCCTCCAAGGC
BglII 810 AGATCT A^GATCT
1242 AGATCT
BsaHI 1909 GACGCC G[GA]^CG[CT]C
2101 GACGCC
BsmAI 2432 GAGAC GTCTC(1/5)
2884 GAGAC
BsmI 1464 GAATGC GAATGC(1/-1)
2238 GAATGC
BsoBI 2423 CCCGAG C^[CT]CG[GA]G
2816 CTCGGG
BsrFI 59 GCCGGC [GA]^CCGG[CT]
2839 GCCGGC
BstEII 1473 GGTGACC G^GT[ACGT]ACC
3096 GGTAACC
BstPI 1473 GGTGACC G^GT[ACGT]ACC
3096 GGTAACC
BstUI 64 CGCG CG^CG
97 CGCG
Cfr10I 59 GCCGGC [GA]^CCGG[CT]
2839 GCCGGC
DraII 2339 GGGACCT [GA]G^G[ACGT]CC[CT]
2525 AGGACCT
EcoO109I 2339 GGGACCT [GA]G^G[ACGT]CC[CT]
2525 AGGACCT
EcoO65I 1473 GGTGACC G^GT[ACGT]ACC
3096 GGTAACC
Hin1I 1909 GACGCC G[GA]^CG[CT]C
2101 GACGCC
HincII 238 GTCAAC GT[CT]^[GA]AC
549 GTTAAC
HindII 238 GTCAAC GT[CT]^[GA]AC
549 GTTAAC
MvnI 64 CGCG CG^CG
97 CGCG
NaeI 59 GCCGGC GCC^GGC
2839 GCCGGC
NgoMIV 59 GCCGGC G^CCGGC
2839 GCCGGC
PpuMI 2339 GGGACCT [GA]G^G[AT]CC[CT]
2525 AGGACCT
SacI 1315 GAGCTC GAGCT^C
2296 GAGCTC
XcmI 2072 CCATAACCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2648 CCACTGTCATGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BalI
BanI BanII BbsI BbvI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DrdI DsaI EaeI EarI
EcoNI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII